Mendelian inheritance, genetic linkage, and genotypic disequilibrium for nine microsatellite loci in Cariniana estrellensis (Raddi) Kuntze (Lecythidaceae)

Kubota, Thaisa Y. K.; Silva, A.M.; Cambuim, José; Silva, A.A.; Pupin, Silvelise; Silva, M.S.; Moraes, Marcela Aparecida de; Moraes, M. L. T.; Sebbenn, Alexandre Magno

doi:10.4238/gmr16029653

Cited by 3 publications

(1 citation statement)

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“…30 Details on amplification and genotyping of the selected loci for C. estrellensis are given in Guidugli et al 29 & Tambarussi et al 30 For C. legalis, details are described in Tambarussi et al 30 All loci used for both species present Mendelian inheritance, an absence of genetic linkage, and genotypic linkage equilibrium. 31,32 Figure 1 Spatial distribution of Cariniana estrellensis and Cariniana legalis trees sampled in the three study areas: Ibicatu State Forest and Mogi-Guaçu State Forest, Sao Paulo State; and Bataguassu, Mato Grosso do Sul State, Brazil. Source: Google Earth, 2018.…”

Section: Dna Extraction and Microsatellite Amplificationmentioning

confidence: 99%

Historic pollen and seed dispersal in fragmented populations of the two largest trees of the atlantic forest

BSouza

Kubota

Tambarussi

et al. 2018

FREIJ

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Cariniana estrellensis and C. legalis are two of the largest trees in the Amazon and Atlantic Forest biomes that are currently vulnerable to extinction due to the intense deforestation of these biomes. Strategies for in and ex situ conservation are urgent and studies of genetic diversity and gene flow are necessary to inform such strategies. Here we investigate the historic pollen and seed flow, dispersal distance and patterns in fragmented populations of both species, using microsatellite markers. All trees found in the populations were mapped, measured for diameter at breast height (DBH), and bark cambium sampled. For both species, high levels of seed (38.5-61.5%) and pollen (80.1-100%) immigration were observed, showing that populations are not genetically isolated. No self-fertilization was detected, but we did find evidence of mating among related trees (8.9-12.5%), suggesting stronger selection against selfed individuals than those originating from mating among relatives. The realized pollen and seed dispersal for both species reached long distances (C. estrellensis > 3 km, C. legalis up to 385 m), but in general followed a pattern of isolation by distance. The effective size (e N) in three populations (10-33) was lower than suggested for short term in situ genetic conservation (e N < 70). For in situ conservation, the results show that the surrounding forest fragments must be preserved to maintain connectivity between the studied populations and other trees of the species and where the e N was insufficient for short term in situ genetic conservation, the e N must be increased through the introduction of at least 150 individuals.

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