Comparative cytogenetic analysis between Lonchorhina aurita and Trachops cirrhosus (Chiroptera, Phyllostomidae)

Abstract

Phyllostomidae comprises the most diverse family of neotropical bats, its wide range of morphological features leading to uncertainty regarding phylogenetic relationships. Seeing that cytogenetics is one of the fields capable of providing support for currently adopted classifications through the use of several markers, a comparative analysis between two Phyllostomidae species was undertaken in the present study, with a view to supplying datasets for the further establishment of Phyllostomidae evolutionary relationships. Karyotypes of Lonchorhina aurita (2n = 32; FN = 60) and Trachops cirrhosus (2n = 30; FN = 56) were analyzed by G- and C-banding, silver nitrate staining (Ag-NOR) and base-specific fluorochromes. Chromosomal data obtained for both species are in agreement with those previously described, except for X chromosome morphology in T. cirrhosus, hence indicating chromosomal geographical variation in this species. A comparison of G-banding permitted the identification of homeologies in nearly all the chromosomes. Furthermore, C-banding and Ag-NOR patterns were comparable to what has already been observed in the family. In both species CMA3 /DA/DAPI staining revealed an R-banding-like pattern with CMA 3 , whereas DAPI showed uniform staining in all the chromosomes. Fluorochrome staining patterns for pericentromeric constitutive heterochromatin (CH) regions, as well as for nucleolar organizing regions (NORs), indicated heterogeneity regarding these sequences among Phyllostomidae species.

Cytogenetics and DNA barcoding of the Round-eared bats, Tonatia (Chiroptera: Phyllostomidae): a new karyotype for Tonatia bidens

“…The Ag-NORs described here for the new karyotype of T. bidens were observed on the smallest pair of acrocentric chromosomes (pair 13). The constitutive heterochromatin found in the pericentromeric region of the chromosomes of T. bidens and in the pericentromeric and interstitial regions of some of the chromosomes of T. saurophila was consistent with the pattern observed in other studies of phyllostomine bats , MORIELLE-VERSUTE et al 1996, SANTOS et al 2001, SILVA et al 2005, BARROS et al 2009, GOMES et al 2010.…”

Integration of molecular cytogenetics, dated molecular phylogeny, and model-based predictions to understand the extreme chromosome reorganization in the Neotropical genus Tonatia (Chiroptera: Phyllostomidae)

“…4 ). Three Phyllostominae synapomorphies, inversion (i) of MCA 4, 12 + 2qi, and 17 + 18) were confirmed by G-band comparison with the karyotype of Trachops cirrhosus (Phyllostominae) as a Phyllostomini outgroup [ 42 ].…”

Cytotaxonomy of the subgenus Artibeus (Phyllostomidae, Chiroptera) by characterization of species-specific markers

“…The CH distribution was evaluated and intercompared in the large Artibeus and with others phyllostomatids, pointing out an extensive similarity of CH pattern local ized in the pericentromeric region ( Rodrigues et al 2000 , Barros et al 2009 , Sbragia et al 2010 ). Additionally, CH blocks were also found in the terminal region of chromosome pairs 5, 6 and 7 that has been considered a characteristic shared by subfamily Stenodermatinae ( Souza and Araújo 1990 , Santos and Souza 1998b , Silva et al 2005 ).…”