Bovine gene polymorphisms related to fat deposition and meat tenderness

Fortes, M. R. S.; Curi, Rogério Abdallah; Chardulo, Luis Artur Loyola; Silveira, Antônio Carlos; Assumpção, Mayra Elena Ortiz D'Ávila; Visintin, José Antônio; Oliveira, Henrique Nunes de

doi:10.1590/s1415-47572009000100011

Cited by 42 publications

(45 citation statements)

References 32 publications

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“…The groups with a higher Bos taurus taurus genetic composition (CRAN, CRVN, CRCN and RACN) exhibited frequencies of 13.8%, 17.3%, 19.6% and 28.6%, respectively ( Table 2). These results are in accordance with those found by Casas et al (2005), who reported a low frequency of allele T in SNP TG5 in Brahman cattle, as well as with the observations of Fortes et al (2009), who reported that allele T (SNP TG5) is fixed in the Nellore breed. These authors concluded that the increase in allele T frequency was associated with the increased Bos taurus taurus genetic composition in the population studied: Rubia Gallega × Nellore (1.9%), three-way cross Brangus (15.8%), Canchim (22%) and three-way cross Braunvieh (33.3%).…”

Section: Resultssupporting

confidence: 93%

“…The genetic group CCVN was the only group in which animals with the TT genotype were not found (Table 3). Fortes et al (2009) assessed the association of polymorphisms in the LEP (AY138588.1:c.305C>T), TG (X05380.1:g.-422C>T) and DGAT1 (AJ318490: c.[10433G>A; 10434C>A]) genes with fat thickness, total lipids, marbling, ribeye area and shear force in 147 animals from five distinct genetic groups (Nellore, Rubia Gallega × Nellore, Canchim, and three-way cross Brangus and Braunvieh). Frequencies of 81% (allele C) and 19% (allele T) were found for the polymorphism E2FB.…”

Section: Resultsmentioning

confidence: 99%

“…Allele T of SNP TG5 is recognized for enabling marbling and carcass fat deposition (Barendse, 1999;Thaller et al, 2003;Barendse et al, 2004;Fortes et al, 2009;Pannier et al, 2010). Of the 201 animals studied, 142 exhibited the genotype CC, 53 the genotype CT and six the genotype TT.…”

Section: Resultsmentioning

confidence: 99%

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Association of polymorphisms in the leptin and thyroglobulin genes with meat quality and carcass traits in beef cattle

et al. 2012

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-The objective of the present study was to estimate the allelic and genotypic frequencies of the polymorphisms E2FB (AY138588.1: c.305C> T), located in the leptin gene (LEP), and TG5 (X05380.1:g.-422C>T), located in the thyroglobulin gene (TG), and evaluate the association of these polymorphisms in crossbred cattle of seven distinct genetic groups with the following traits: slaughter weight (SW), hot carcass weight (HCW), hot carcass yield (HCY), carcass fat thickness (CFT), ribeye area (REA), marbling (MARM) and shear force (SF). The animals were genotyped using the PCR-RFLP (Polymorphism Chain Reaction-Restriction Fragment Length Polymorphism) technique, using 201 products obtained from F 1 Caracu × Nellore, Angus × Nellore and Valdostana × Nellore cows, mated to Canchim, Caracu and Red Angus bulls (only Caracu × Nellore cows were used with Red Angus bulls). The allelic and genotypic frequencies were compared using the Chi-squared test. Associations between the genotype of each polymorphism and the traits were analyzed using the General Linear Model (GLM) of statistical software SAS. The least squares means of genotypes of the polymorphisms were compared using Student's t test. The E2FB polymorphism in the LEP gene was associated with CFT, showing the potential for use in national programs for genetic improvement of beef cattle, through the inclusion of SNP in genotyping commercial tests. The TG5 polymorphism in the TG gene was not associated with any of the evaluated traits and was considered ineffective for selection of beef cattle in Brazilian herds.

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

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Association of polymorphisms in the leptin and thyroglobulin genes with meat quality and carcass traits in beef cattle

et al. 2012

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“…It showed that C allele of TG5 was the most common allele found in all cattle breeds. However, C allele was more frequent found in Bos indicus breeds such as Brahman cattle (Casas et al, 2005) and Nelore cattle (Fortes et al, 2009) and tend to be fixed as well as in Bos javanicus than in Bos taurus breeds. Conversely, the T allele was mostly frequent in Bos taurus breeds (0.229) compared to Bos indicus (0.020) and Bos javanicus (0.002), although the frequency of T allele in Bos taurus was varied (0.016-0.487).…”

Section: Genetic Polymorphism Analysismentioning

confidence: 89%

“…As the results in this study, the other new genetic markers may be explored as well as Fortes et al (2009) suggestion from their study in Nelore cattle. Gan et al (2008) and Zang et al (2015) have reported any SNPs within TG gene in beef cattle.…”

Section: Bos Taurusmentioning

confidence: 92%

Genetic polymorphism analysis of 5' untranslated region of thyroglobulin gene in Bali cattle (Bos javanicus) from three different regions of Indonesia

Anwar

Putra

Wulandari

et al. 2017

J. Indonesian Trop. Anim. Agric.

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ABSTRAKVariasi nukleotida g.422C>T pada gen thyroglobulin dibagian 5'untranslated region (5'UTR) (disebut TG5) telah dilaporkan mempunyai asosiasi dengan tingkat kandungan lemak intramuskuler atau marbling pada sapi potong. Penelitian ini ditujukan untuk mengkonfirmasi keragaman genetik gen TG5 pada sapi Bali dari tiga wilayah sumber bibit yang berbeda di Indonesia. Sebanyak 200 ekor sapi Bali telah dilakukan genotyping pada gen TG5 menggunakan metode polymerase chain reaction-restriction fragment lenght polymorphism (PCR-RFLP) dan analisis sekuen. Hasil penelitian menunjukkan bahwa TG5 terkonfirmasi monomorfik pada sapi Bali dimanapun wilayah asalnya. Selain itu, sebanyak sembilan kandidat SNP ditemukan di dalam gen TG bagian 5'UTR pada sapi Bali dibandingkan dengan sekuens Genbank, meskipun tidak ditemukan variasi SNP di dalam sampel sapi Bali yang diteliti. Marker genetik lain dari gen TG perlu dieksplorasi dan diverifikasi keragamannya pada sapi Bali. Sembilan kandidat SNP juga perlu diverifikasi dan divalidasi lebih lanjut dengan sampel yang lebih besar untuk dapat dikatakan sebagai SNP baru antara sapi Bali dengan referensi sekuen dari GenBank. Kata kunci : gen thyroglobulin, marbling, polimorfisme genetik, sapi Bali, 5'-untranslated region ABSTRACTThe g.422C>T nucleotide variations in the 5' untranslated region (5'UTR) of TG gene (called as TG5) has been reported to be associated with level in intramuscular fat (IMF) content or marbling in beef cattle. The objective of this study was to confirm genetic polymorphism of TG5 gene in Bali cattle populations from three different regions as the main resources of Bali cattle in Indonesia. A total of 200 head of Bali cattle have been performed genotyping on TG5 gene using polymerase chain reactionrestriction fragment lenght polymorphism (PCR-RFLP) method and sequence analysis. Results of the study confirmed that TG5 was monomorphic in Bali cattle wherever their origin regions. Moreover, nine candidate SNPs were detected within 5'UTR of TG gene in Bali cattle compared to Genbank reference sequences, although no SNP variations among Bali cattle sample studied. The new other genetic markers within an entire TG gene suggested to be explored and verified for their polymorphisms in Bali cattle. (S. Anwar et al.) 5'UTR of Thyroglobulin Gene Polymorphism in Bali Cattle 175The nine candidate SNPs were also required further verification and validation in a larger sample to be regarded as new SNPs between Bali cattle and Genbank reference sequences.

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Annotation of novel transcripts putatively relevant for bovine fat metabolism

et al. 2010

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Two bovine transcripts encoded by the interleukin-1 receptor-associated kinase 1 (IRAK1) gene and the locus LOC618944 predicted as similar to human chromosome 6 open reading frame 52 (C6orf52) gene had indicated divergent expression in bovine skeletal muscle containing different amount of intramuscular fat in a pilot screening experiment. However, for both loci any role in the regulation of energy or fat metabolism is not yet described. In this study, we validated and refined gene structure, screened for mRNA splice variants and analyzed the tissue-specific gene expression patterns of both loci as a prerequisite to elucidate their potential physiological function. Based on comparative sequence analysis, a new full-length gene model for the bovine IRAK1 gene was developed and confirmed experimentally. Expression of IRAK1 mRNA was found in a variety of tissues, and a splice variant was identified in skeletal muscle caused by an in-frame deleted segment of 210 bp affecting regions of intrinsic disorder in the respective protein. For the locus LOC618944, our data contributed to a revised gene model and its assignment to BTA23 (bovine chromosome 23) on the current bovine genome assembly supported by comparative similarity analysis between the bovine and human genomes and experimental data. Furthermore, we identified several splice variants in mammary gland, fat and skeletal muscle tissue and detected a highly similar processed pseudogene on BTA26. All transcript variants of LOC618944 detected in the analyzed tissues represent noncoding RNAs. For both loci, our results suggest yet undetected physiological functions in tissues relevant for fat or energy metabolism in cattle.

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Bovine gene polymorphisms related to fat deposition and meat tenderness

Cited by 42 publications

References 32 publications

Association of polymorphisms in the leptin and thyroglobulin genes with meat quality and carcass traits in beef cattle

Association of polymorphisms in the leptin and thyroglobulin genes with meat quality and carcass traits in beef cattle

Genetic polymorphism analysis of 5' untranslated region of thyroglobulin gene in Bali cattle (Bos javanicus) from three different regions of Indonesia

Annotation of novel transcripts putatively relevant for bovine fat metabolism

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