Autonomous growth of BALB/MK keratinocytes transfected with a retroviral vector carrying the human epidermal growth factor gene

Abstract

Epidermal growth factor (EGF), which promotes epidermal regeneration and wound closure, is important for the proliferation and differentiation of epidermal and epithelial tissues in animals. Exogenous EGF is a promising therapeutic agent for wound healing, but its general use is restricted by the limited availability of this protein. In this work, we show that the transfection of mouse BALB/MK keratinocytes, which are totally dependent on EGF for growth and migration, with mature cDNA for human EGF via a retroviral vector abolished the cells requirement for exogenous EGF. The transformed cells had normal morphology and a growth rate that varied according to the source of the retroviral vector used. Keratinocyte transfection with EGF cDNA provides a time-and cost-efficient means of culturing keratinocytes and yields cells that may be useful for skin grafting.

IAEA activities related to radiation biology and health effects of radiation

“…Since the initiation of this CRP in 2008, considerable progress has been made regarding the development of treatment protocols for the amelioration of radiation-induced damage to normal tissues. For example, regarding the treatment of radiation ulcers after exposure of the skin to high doses of radiation, three different approaches were presented: (1) the use of a skin regenerating template (chitosan) seeded with keratinocytes [4]; (2) the construction of a dermal-epidermal biological matrix using skin stem cells and virally transformed keratinocytes producing growth factors [5,6];…”

Comparação do efeito antiangiogênico do ranibizumab e do bevacizumab in vitro

“…Foram realizadas três medidas por campo, gerando uma mediada média por campo. Estas medidas foram analisadas por GraphPad Prism 5.0 (GraphPad Software, Inc) utilizando o teste T-student (16) .…”