A phylogenetic analysis of Brycon and Henochilus (Characiformes, Characidae, Bryconinae) based on the mitochondrial gene 16S rRNA

Hilsdorf, Silva; Oliveira, Cláudio; Lima, Flávio C. T.; Matsumoto, Cristianne Kayoko

doi:10.1590/s1415-47572008000200034

Cited by 11 publications

(17 citation statements)

References 19 publications

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“…Géry [15], without a phylogenetic analyses, included the tribe Salminini in the subfamily Bryconinae. Our study corroborates previous molecular hypothesis that identify Salminus as closely related to Brycon [1,20,21,51,60]. Moreover, our results show that Salminus is a genus interspersed among Brycon species, as observed by Calcagnotto et al [20].…”

Section: Discussionsupporting

confidence: 93%

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Systematic and historical biogeography of the Bryconidae (Ostariophysi: Characiformes) suggesting a new rearrangement of its genera and an old origin of Mesoamerican ichthyofauna

et al. 2014

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BackgroundRecent molecular hypotheses suggest that some traditional suprageneric taxa of Characiformes require revision, as they may not constitute monophyletic groups. This is the case for the Bryconidae. Various studies have proposed that this family (considered a subfamily by some authors) may be composed of different genera. However, until now, no phylogenetic study of all putative genera has been conducted.ResultsIn the present study, we analyzed 27 species (46 specimens) of all currently recognized genera of the Bryconidae (ingroup) and 208 species representing all other families and most genera of the Characiformes (outgroup). Five genes were sequenced: 16SrRNA, Cytochrome b, recombination activating gene 1 and 2 and myosin heavy chain 6 cardiac muscle. The final matrix contained 4699 bp and was analyzed by maximum likelihood, maximum parsimony and Bayesian analyses. The results show that the Bryconidae, composed of Brycon, Chilobrycon, Henochilus and Salminus, is monophyletic and is the sister group of Gasteropelecidae + Triportheidae. However, the genus Brycon is polyphyletic. Fossil studies suggest that the family originated approximately 47 million years ago (Ma) and that one of the two main lineages persisted only in trans-Andean rivers, including Central American rivers, suggesting a much older origin of Mesoamerican ichthyofauna than previously accepted.ConclusionBryconidae is composed by five main clades, including the genera Brycon, Chilobrycon, Henochilus and Salminus, but a taxonomic review of these groups is needed. Our results point to a possible ancient invasion of Central America, dating about 20.3 ± 5.0 Ma (late Oligocene - early Miocene), to explain the occurrence of Brycon in Central America.

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Section: Discussionsupporting

confidence: 93%

“…Their results indicated a close relationship between Brycon and Henochilus , as did those of Hilsdorf et al [60]. Henochilus is morphologically very similar to Brycon and Chilobrycon , with fewer tooth series.…”

Section: Discussionsupporting

confidence: 60%

Systematic and historical biogeography of the Bryconidae (Ostariophysi: Characiformes) suggesting a new rearrangement of its genera and an old origin of Mesoamerican ichthyofauna

et al. 2014

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“…Based on molecular data, Hilsdorf and colleagues [10] obtained some well-supported molecular clades. Within the eastern coastal clade, H. wheatlandii is the sister species of Brycon insignis and Brycon ferox .…”

Section: Discussionmentioning

confidence: 99%

The Unique Karyotype of Henochilus wheatlandii, a Critically Endangered Fish Living in a Fast-Developing Region in Minas Gerais State, Brazil

et al. 2012

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Henochilus wheatlandii, the only species of this genus, is critically endangered and was considered extinct for over a century. The rediscovery of this fish in 1996 made it possible to study its phylogenetic relationships with other species in the subfamily Bryconinae. The aim of this study was to characterise the karyotype of H. wheatlandii. Standard staining, C-positive heterochromatin and nucleolar organiser region (NOR) banding, chromomycin A3 staining, and fluorescent in situ hybridisation (FISH) using 5S rDNA and 18S rDNA probes were conducted on nineteen specimens collected in the Santo Antonio River, a sub-basin of the Doce River in Ferros municipality, Minas Gerais State, Brazil. Henochilus wheatlandii shared the same diploid number and chromosome morphology as other species of Bryconinae. However, its heterochromatin distribution patterns, NOR localisation, and FISH patterns revealed a cytogenetic profile unique among Neotropical Bryconinae, emphasizing the evolutionary uniqueness of this threatened species.

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“…Besides, the results demonstrated that the species of the genus Brycon belong to a monophyletic group. Phylogenetic studies with B. pesu, B. hilarii, B. orbignyanus, and other Brycon species, based on the mitochondrial gene 16S rRNA, also supported the monophyletic status of the Brycon species, although considered the paraphyletic status of the genus (Hilsdorf et al, 2008).…”

Section: Discussionmentioning

confidence: 81%

“…Such environments, with similar characteristics, allowed the origin of species with similar habits and morphological features, such as B. hilarii and B. orbignyanus. Hilsdorf et al (2008) According to the results, B. cephalus and B. hilarii diverged from a common ancestor, that branched out into two lineages: the older originated B. cephalus, and the most recent was divided into two branches. One originated B. orbignyanus and the second originated B. hilarii.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization and phylogenetic relationships among species of the genus Brycon (Characiformes: Characidae) from four hydrographic basins in Brazil

Antunes¹,

Gomes²,

Prioli³

et al. 2010

Genet. Mol. Res.

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ABSTRACT. Brycon is one of the main genera of Neotropical freshwater fish. In Brazil, Brycon species have been found in many hydrographic basins, such as the Amazon, Paraná, Paraguay, and AraguaiaTocantins basins. We examined the phylogenetic relationships among the species Brycon orbignyanus, B. hilarii, B. cf. pesu, B. cephalus, B. falcatus, and B. gouldingi, using mitochondrial and nuclear molecular markers. Specimens of B. orbignyanus were collected in the Paraná River. Specimens of B. hilarii were collected in the Manso River. Specimens of B. cephalus were obtained from a fish farm, and 675 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 9 (2): 674-684 (2010) Phylogenetic relationships among Brycon species specimens of B. cf. pesu, B. falcatus and B. gouldingi were sampled in the Araguaia-Tocantins basin. DNA extraction was carried out using the phenol/chloroform method. Molecular polymorphism studies of Brycon species were carried out with the inter-simple sequence repeat (ISSR) technique, using the total DNA of six specimens of each species. In DNA amplification of B. cf. pesu, eight specimens were used. The partial sequence of mitochondrial cytochrome b was amplified by PCR. The PCR products were used directly in sequencing reactions. Each ISSR primer produced from 7 to 14 scorable and reproducible bands. The (GGAC) 3 A and (GGAC) 3 C primers produced the greatest number of species-specific bands. A 264-bp fragment, corresponding to the partial region of mitochondrial DNA cytochrome b, was sequenced and used for analysis. According to the phylogenetic tree obtained from the data, these Brycon species can be divided into two clades: one comprised only B. cf. pesu, and the second comprised the remaining Brycon species. We conclude that ISSR primers can be used for the identification of species-specific bands in fish, such as Brycon spp.

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A phylogenetic analysis of Brycon and Henochilus (Characiformes, Characidae, Bryconinae) based on the mitochondrial gene 16S rRNA

Cited by 11 publications

References 19 publications

Systematic and historical biogeography of the Bryconidae (Ostariophysi: Characiformes) suggesting a new rearrangement of its genera and an old origin of Mesoamerican ichthyofauna

Systematic and historical biogeography of the Bryconidae (Ostariophysi: Characiformes) suggesting a new rearrangement of its genera and an old origin of Mesoamerican ichthyofauna

The Unique Karyotype of Henochilus wheatlandii, a Critically Endangered Fish Living in a Fast-Developing Region in Minas Gerais State, Brazil

Molecular characterization and phylogenetic relationships among species of the genus Brycon (Characiformes: Characidae) from four hydrographic basins in Brazil

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