Karyotype relationships among Anastrepha bistrigata, A. striata and A. serpentina (Diptera, Tephritidae)

Abstract

The species of Anastrepha are arranged into 17 intrageneric groups. Recently, it was proposed that two species of the striata group, Anastrepha striata and A. bistrigata, might be realocated to serpentina group. Anastrepha bistrigata and A. serpentina have an X 1 X 2 Y/X 1 X 1 X 2 X 2 sex chromosome system while A. striata has a XY/XX system. It was previously proposed that the karyotype of A. bistrigata could be derived from that of A. striata by an Y:A fusion, and that the karyotype of A. serpentina would be derived from another, hypothetical karyotype. In the present report sequential staining with DAPI and chromomycin A3 (CMA 3 ), followed by C-banding, revealed that the C-banded heterochromatic blocks of the sex chromosomes of A. bistrigata have different affinities to fluorochromes in comparison to the chromosomes of A. striata, from which they have hypothetically derived. The chromosomes of A. serpentina show substantial differences in their cytochemical properties compared to their A. bistrigata and A. striata counterparts. The FISH technique showed that the ribosomal gene sequences are located in DAPI-or DAPI/CMA 3 -positive heterochromatic blocks of the sex chromosomes, one site on the Y chromosome and one site on the X chromosome (X 1 in A. bistrigata and A. serpentina). The data suggest that the karyotype of A. striata and A. bistrigata could be derived from a common ancestral karyotype, while the A. serpentina karyotype probably has a distinct origin.

A review of more than 30 years of cytogenetic studies of Tephritidae in support of sterile insect technique and global trade

“…All members of the A. fraterculus intrageneric group are characterized by acrocentric chromosomes (Selivon et al., ; Goday et al., ; Cáceres et al., ). However, out of this group, there are Anastrepha species with (1) total chromosome number 2n = 12 with submetacentric or a combination of submetacentric and rod‐shaped chromosomes and (2) different number of total chromosomes such as Anastrepha pickeli Lima with 2n = 8 (XX/XY), Anastrepha leptozona Hendel with 2n = 10 (XX/XY) or different numbers of sex chromosomes, such as Anastrepha bistrigata Bezzi and Anastrepha serpentina (Wiedemann) with a karyotype of 2n = 11 for males and 2n = 12 for females (X 1 X 2 Y/X 1 X 2 X 1 X 2 ) (Selivon et al., , ).…”

Cytogenetic and symbiont analysis of five members of the B. dorsalis complex (Diptera, Tephritidae): no evidence of chromosomal or symbiont-based speciation events

“…In Tephritids, there is a number of studies presenting and discussing mitotic karyotypes, especially for Bactrocera (Hunwattanakul and Baimai 1994, Baimai et al 1995, 1999, 2000, Baimai 1998, 2000), Anastrepha (Cevallos and Nation 2004, Selivon et al 2005, Goday et al 2006, Selivon et al 2007) and Rhagoletis species (Bush and Boller 1977). However, useful polytene chromosome maps, so far available for five genera, represent only 11 species: one of Anastrepha ( Anastrepha ludens ) (Garcia-Martinez et al 2009), one of Ceratitis ( Ceratitis capitata ) (Zacharopoulou 1990), one of Dacus ( Dacus ciliatus ) (Drosopoulou et al 2011b) and three of Rhagoletis , namely Rhagoletis cerasi (Kounatidis et al 2008), Rhagoletis cingulata (Drosopoulou et al 2011a) and Rhagoletis completa (Drosopoulou et al 2010).…”