Bioinformatics for the Citrus EST Project (CitEST)

Reis, Marcelo S.; Takita, Marco Aurélio; Palmieri, Darío Abel; Machado, Marcos Antônio

doi:10.1590/s1415-47572007000500031

Cited by 7 publications

(5 citation statements)

References 14 publications

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“…The Citrus Functional Genomics Project (CFGP; http://citrusge nomics.ibmcp-ivia.upv.es) and the CitEST website (http://biotec nologia.centrodecitricultura.br) are two of the available websites developed on the basis of the genomic information available for Citrus and its relatives. The first is a genomic tool for large-scale studies of biological processes relevant to citriculture, such as developmental biology, biotic and abiotic stresses and postharvest processes (Forment et al, 2005), while the second is a provider for several web services, related to sequence submission and data mining (Reis et al, 2007). Today Citrus transcriptome is well-documented, thanks to sequenced citrus ESTs included in several open database projects (http://harvest.ucr.edu/; http://cgf.…”

Section: Introductionmentioning

confidence: 99%

Use of a custom array to study differentially expressed genes during blood orange (Citrus sinensis L. Osbeck) ripening

Bernardi

Licciardello²,

Russo³

et al. 2010

Journal of Plant Physiology

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Section: Introductionmentioning

confidence: 99%

Use of a custom array to study differentially expressed genes during blood orange (Citrus sinensis L. Osbeck) ripening

Bernardi

Licciardello²,

Russo³

et al. 2010

Journal of Plant Physiology

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“…Collaborative projects to generate genomic resources for Citrus, including EST libraries from various organs and cDNA microarrays (Forment et al, 2005;Reis et al, 2007), have yielded data sets that have been mined by several groups to discover genes with potential relevance to essential oil biosynthesis in the peel. For example, transcripts related to terpenoid synthases, which catalyze the first committed step in the biosynthesis of terpenoid essential oil constituents, were highly expressed in Citrus fruit peel when compared with other tissues and organs Dornelas and Mazzafera, 2007;Takita et al, 2007).…”

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confidence: 99%

Assessing the Biosynthetic Capabilities of Secretory Glands inCitrusPeel

2012

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Epithelial cells (ECs) lining the secretory cavities of Citrus peel have been hypothesized to be responsible for the synthesis of essential oil, but direct evidence for such a role is currently sparse. We used laser-capture microdissection and pressure catapulting to isolate ECs and parenchyma cells (as controls not synthesizing oil) from the peel of young grapefruit (Citrus × paradisi ‘Duncan’), isolated RNA, and evaluated transcript patterns based on oligonucleotide microarrays. A Gene Ontology analysis of these data sets indicated an enrichment of genes involved in the biosynthesis of volatile terpenoids and nonvolatile phenylpropanoids in ECs (when compared with parenchyma cells), thus indicating a significant metabolic specialization in this cell type. The gene expression patterns in ECs were consistent with the accumulation of the major essential oil constituents (monoterpenes, prenylated coumarins, and polymethoxylated flavonoids). Morphometric analyses demonstrated that secretory cavities are formed early during fruit development, whereas the expansion of cavities, and thus oil accumulation, correlates with later stages of fruit expansion. Our studies have laid the methodological and experimental groundwork for a vastly improved knowledge of the as yet poorly understood processes controlling essential oil biosynthesis in Citrus peel.

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“…Twenty eight antimicrobial peptides were prospected, twenty two of which were from CitEST (citrus expressed genome) [ 25 ] Database, with 110,903 unigenes and six were selected from the complete genomes of C . sinensis (46,147 proteins) and C .…”

Section: Methodsmentioning

confidence: 99%

Evaluation of cytotoxicity features of antimicrobial peptides with potential to control bacterial diseases of citrus

et al. 2018

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Antimicrobial peptides (AMPs) can be found in various organisms, and could be considered an alternative for pesticides used to control plant pathogens, including those affecting citrus. Brazil is the largest producer and exporter of frozen concentrated orange juice in the world. However, the citrus industry has been affected by several diseases such as citrus canker and huanglongbing (HLB), caused by the bacteria Xanthomonas citri subsp. citri (X.citri) and Candidatus Liberibacter asiaticus (CaLas), respectively. In order to control these pathogens, putative AMPs were prospected in databases containing citrus sequences. Furthermore, AMPs already reported in the literature were also used for in vitro and in vivo assays against X.citri. Since CaLas cannot be cultivated in vitro, surrogates as Sinorhizobium meliloti and Agrobacterium tumefaciens were used. This study reports the evaluation of six AMPs obtained from different sources, two of them from Citrus spp. (citrus-amp1 and citrus-amp2), three from amphibians (Hylin-a1, K0-W6-Hy-a1 and Ocellatin 4-analogue) and one from porcine (Tritrpticin). Peptides K0-W6-Hy-a1, Ocellatin 4-analogue, and citrus-amp1 showed bactericidal activity against X.citri and S. meliloti and bacteriostatic effect on A. tumefaciens. These results were confirmed for X.citri in planta. In addition cytotoxicity evaluations of these molecules were performed. The AMPs that showed the lowest hemolytic activities were Triptrpticin, citrus-amp1 and citrus-amp2. Citrus-amp1 and citrus-amp2 not presented toxicity in experiments using in vivo model, G. mellonella and U87 MG cells. To verify the interaction of these AMPs with bacteria and erythrocyte cell membranes, vesicles mimicking these cells were built. Citrus-amp1 and Tritrpticin exhibited higher affinity to bacterial membranes, while Ocellatin 4-analogue and Hylin-a1 showed higher affinity to erythrocyte membranes; exclude their use in citrus. This work demonstrates an essential alternative, trough AMPs obtained from Citrus spp., which can be feasibly used to control bacterial pathogens.

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Bioinformatics for the Citrus EST Project (CitEST)

Cited by 7 publications

References 14 publications

Use of a custom array to study differentially expressed genes during blood orange (Citrus sinensis L. Osbeck) ripening

Use of a custom array to study differentially expressed genes during blood orange (Citrus sinensis L. Osbeck) ripening

Assessing the Biosynthetic Capabilities of Secretory Glands inCitrusPeel

Evaluation of cytotoxicity features of antimicrobial peptides with potential to control bacterial diseases of citrus

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