Use of a custom array to study differentially expressed genes during blood orange (Citrus sinensis L. Osbeck) ripening

Bernardi, Jamila; Licciardello, Concetta; Russo, Mario; Chiusano, Maria Luisa; Carletti, Giorgia; Recupero, Giuseppe Reforgiato; Marocco, Adriano

doi:10.1016/j.jplph.2009.09.009

Cited by 27 publications

(17 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is implied here that physiological processes that produce these essential vitamins are favoured up to the ripening of the fruits. This is in agreement with earlier studies that β-carotene content of ripe tomatoes varied directly with the ripeness of the fruit at harvest [16]. It is significant to observe the high increase in vitamin C as the fruits transform from unripe to ripe stage.…”

Section: Vitamin Content Of the Samplessupporting

confidence: 93%

“…Fruit senescence as well brings about a plethora of deteriorative changes and disorders, all of which contribute to the weakening of the rind and leads to the final demise of the fruit. Most importantly however, senescence is promoted by plant hormones [16]. Plant hormones are chemicals produced by one part of the plant and transported to another part where they exert their action.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Impact of Senescence on the Nutritional Profiles of Some Fruits Consume in Makurdi, Nigeria

Adie

Ediga

Wuana

2019

AJACR

View full text Add to dashboard Cite

Chemical and nutritional changes with senescence in some fruits commonly consumed in Makurdi, North Central Nigeria, were investigated using standard analytical methods. Four fruits: mango, pawpaw, orange and pineapple at three different stages of growth; pre-ripe, ripe, and post-ripe were screened for proximate composition, mineral elements, pH, titrable acidity, and vitamins A and C. Results indicated increase in moisture content as the fruit deteriorated in the climacteric fruits (mango and pawpaw), while in the non-climateric fruits (orange and pineapple) the parameter depreciated from ripe to over-ripe. Other proximate parameters showed a consistent rise at ripening and declining thereafter as the fruit deteriorated. In all cases, pH of the fruits showed steady rise with senescence, and this was inversely related to the titrable acidity. Beta-carotene (vitamin A) and ascorbic acid (vitamin C) showed similar trend of peaking at the ripening stages and declining as the fruits aged. Nitrogen recorded the highest concentration (45.28±0.04 mg/L) among the macronutrient elements at the ripe stage of pawpaw, while phosphorus recorded the least (1.39±0.01 mg/L) for over-ripe pineapple. The instrument failed to detect Ni in all samples analyzed in the micronutrient mineral elements category, while Ca recorded the highest (5.25±0.07 mg/L). Macronutrients showed consistency in appreciating at ripening and depreciating as the fruits aged. Only Zn showed presence in all samples among the micronutrients analyzed. Results showed that in most of the parameters investigated, nutritional and chemical contents of the fruits are at their optimum at the ripen stage of the fruits, and declined as the fruits deteriorates with age.

show abstract

Section: Vitamin Content Of the Samplessupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Impact of Senescence on the Nutritional Profiles of Some Fruits Consume in Makurdi, Nigeria

Adie

Ediga

Wuana

2019

AJACR

View full text Add to dashboard Cite

show abstract

“…TT19 ) from A. thaliana , which is reported to be involved in anthocyanins accumulation [29,35,36]. The over expression of DQ198153 in Moro flesh when compared to Cadenera (Table 2), as well as the tissue specificity of the ESTs this sequence is assembled from, highlight that the sequence is differentially expressed in Moro flesh fruit, and suggest that this GST may be specifically involved in the accumulation of anthocyanins in the blood cultivar [37]. In fact, it is known that although the anthocyanin’s biosynthetic enzymes are localized in the cytoplasm [38], stable colouration develops only after the pigment is transferred to the vacuole thank to a GST, as observed in maize [39].…”

Section: Resultsmentioning

confidence: 99%

Characterization of the glutathione S-transferase gene family through ESTs and expression analyses within common and pigmented cultivars of Citrus sinensis(L.) Osbeck

Licciardello¹,

D’Agostino

Traini

et al. 2014

BMC Plant Biol

Self Cite

View full text Add to dashboard Cite

BackgroundGlutathione S-transferases (GSTs) represent a ubiquitous gene family encoding detoxification enzymes able to recognize reactive electrophilic xenobiotic molecules as well as compounds of endogenous origin. Anthocyanin pigments require GSTs for their transport into the vacuole since their cytoplasmic retention is toxic to the cell. Anthocyanin accumulation in Citrus sinensis (L.) Osbeck fruit flesh determines different phenotypes affecting the typical pigmentation of Sicilian blood oranges. In this paper we describe: i) the characterization of the GST gene family in C. sinensis through a systematic EST analysis; ii) the validation of the EST assembly by exploiting the genome sequences of C. sinensis and C. clementina and their genome annotations; iii) GST gene expression profiling in six tissues/organs and in two different sweet orange cultivars, Cadenera (common) and Moro (pigmented).ResultsWe identified 61 GST transcripts, described the full- or partial-length nature of the sequences and assigned to each sequence the GST class membership exploiting a comparative approach and the classification scheme proposed for plant species. A total of 23 full-length sequences were defined. Fifty-four of the 61 transcripts were successfully aligned to the C. sinensis and C. clementina genomes. Tissue specific expression profiling demonstrated that the expression of some GST transcripts was 'tissue-affected' and cultivar specific. A comparative analysis of C. sinensis GSTs with those from other plant species was also considered. Data from the current analysis are accessible at http://biosrv.cab.unina.it/citrusGST/, with the aim to provide a reference resource for C. sinensis GSTs.ConclusionsThis study aimed at the characterization of the GST gene family in C. sinensis. Based on expression patterns from two different cultivars and on sequence-comparative analyses, we also highlighted that two sequences, a Phi class GST and a Mapeg class GST, could be involved in the conjugation of anthocyanin pigments and in their transport into the vacuole, specifically in fruit flesh of the pigmented cultivar.

show abstract

“…A regulatory complex, composed of proteins of the Myb, basic helix-loop-helix (bHLH), and WD-repeat families of transcription factors, governs the expression of the structural genes required for anthocyanin biosynthesis, modification, and transport (Ramsay and Glover, 2005;Butelli et al, 2008). In blood orange, several anthocyanin biosynthetic genes show increased expression compared with blond orange (Licciardello et al, 2008;Bernardi et al, 2010;Cultrone et al, 2010). Variation in pigment intensity or tissue specificity in plants is governed largely by the activity of the R2R3 Myb transcription factors in the complex (Kobayashi et al, 2004;Schwinn et al, 2006;Takos et al, 2006;Espley et al, 2007;Geekiyanage et al, 2007;Walker et al, 2007).…”

Section: Identification Of a Gene Encoding An R2r3 Myb Transcription mentioning

confidence: 99%

Retrotransposons Control Fruit-Specific, Cold-Dependent Accumulation of Anthocyanins in Blood Oranges

Licciardello²,

et al. 2012

Self Cite

View full text Add to dashboard Cite

Traditionally, Sicilian blood oranges (Citrus sinensis) have been associated with cardiovascular health, and consumption has been shown to prevent obesity in mice fed a high-fat diet. Despite increasing consumer interest in these healthpromoting attributes, production of blood oranges remains unreliable due largely to a dependency on cold for full color formation. We show that Sicilian blood orange arose by insertion of a Copia-like retrotransposon adjacent to a gene encoding Ruby, a MYB transcriptional activator of anthocyanin production. The retrotransposon controls Ruby expression, and cold dependency reflects the induction of the retroelement by stress. A blood orange of Chinese origin results from an independent insertion of a similar retrotransposon, and color formation in its fruit is also cold dependent. Our results suggest that transposition and recombination of retroelements are likely important sources of variation in Citrus.

show abstract

Use of a custom array to study differentially expressed genes during blood orange (Citrus sinensis L. Osbeck) ripening

Cited by 27 publications

References 37 publications

Impact of Senescence on the Nutritional Profiles of Some Fruits Consume in Makurdi, Nigeria

Impact of Senescence on the Nutritional Profiles of Some Fruits Consume in Makurdi, Nigeria

Characterization of the glutathione S-transferase gene family through ESTs and expression analyses within common and pigmented cultivars of Citrus sinensis(L.) Osbeck

Retrotransposons Control Fruit-Specific, Cold-Dependent Accumulation of Anthocyanins in Blood Oranges

Contact Info

Product

Resources

About