A preliminary survey of M. hyopneumoniae virulence factors based on comparative genomic analysis

Ferreira, Henrique Bunselmeyer; Castro, Luiza Amaral de

doi:10.1590/s1415-47572007000200012

Cited by 21 publications

(14 citation statements)

References 58 publications

(68 reference statements)

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“…Sixteen M . hyopneumoniae putative adhesin-coding genes [44] were selected for comparison analysis. In these genes, palindrome repeats (PAL and PALG) were found with an average of 5 repeat elements per gene in M .…”

Section: Resultsmentioning

confidence: 99%

“…flocculare ) as shown in Fig 5. Adhesin proteins are known to be essential to mycoplasma host infection and for the establishment of the disease [44]. To evaluate the correlation of the palindromic repeats associated with these genes, another comparison analysis was done.…”

Section: Discussionmentioning

confidence: 99%

“…The second comparison analysis was performed in genes coding for putative adhesins [44]. Each palindromic repeat (PAL or PALG) already found upstream of the start codon of adhesin-coding genes in M .…”

Section: Methodsmentioning

confidence: 99%

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Repetitive Elements in Mycoplasma hyopneumoniae Transcriptional Regulation

et al. 2016

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Transcriptional regulation, a multiple-step process, is still poorly understood in the important pig pathogen Mycoplasma hyopneumoniae. Basic motifs like promoters and terminators have already been described, but no other cis-regulatory elements have been found. DNA repeat sequences have been shown to be an interesting potential source of cis-regulatory elements. In this work, a genome-wide search for tandem and palindromic repetitive elements was performed in the intergenic regions of all coding sequences from M. hyopneumoniae strain 7448. Computational analysis demonstrated the presence of 144 tandem repeats and 1,171 palindromic elements. The DNA repeat sequences were distributed within the 5’ upstream regions of 86% of transcriptional units of M. hyopneumoniae strain 7448. Comparative analysis between distinct repetitive sequences found in related mycoplasma genomes demonstrated different percentages of conservation among pathogenic and nonpathogenic strains. qPCR assays revealed differential expression among genes showing variable numbers of repetitive elements. In addition, repeats found in 206 genes already described to be differentially regulated under different culture conditions of M. hyopneumoniae strain 232 showed almost 80% conservation in relation to M. hyopneumoniae strain 7448 repeats. Altogether, these findings suggest a potential regulatory role of tandem and palindromic DNA repeats in the M. hyopneumoniae transcriptional profile.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Repetitive Elements in Mycoplasma hyopneumoniae Transcriptional Regulation

et al. 2016

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“…Comparisons between the genomes of M. hyopneumoniae pathogenic and non-pathogenic strains (7448 and J, respectively) revealed no extensive genomic differences [7]. Moreover, previous comparative phylogenetic and phylogenomic studies provided evidences of the close relationship of M. hyopneumoniae and M. flocculare [7–9], which share most of the known virulence-related genes [10]. The differences between M. hyopneumoniae and M. flocculare include the absence, in M. flocculare , of the glpO gene, related to M. hyopneumoniae hydrogen peroxide generation and cytotoxicity [11,12], and differential domains between orthologs from the P97 family of adhesins and from other surface proteins [13].…”

Section: Introductionmentioning

confidence: 99%

Comparative proteomics of two Mycoplasma hyopneumoniae strains and Mycoplasma flocculare identified potential porcine enzootic pneumonia determinants

et al. 2018

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Mycoplasma hyopneumoniae and Mycoplasma flocculare are genetically similar bacteria, which coinhabit the porcine respiratory tract. These mycoplasmas share most of the known virulence factors, but, while M. hyopneumoniae causes porcine enzootic pneumonia (PEP), M. flocculare is a commensal species. To identify potential PEP determinants and provide novel insights on mycoplasma-host interactions, the whole cell proteomes of two M. hyopneumoniae strains, one pathogenic (7448) and other non-pathogenic (J), and M. flocculare were compared. A cell fractioning approach combined with mass spectrometry (LC-MS/MS) proteomics was used to analyze cytoplasmic and surface-enriched protein fractions. Average detection of ~ 50% of the predicted proteomes of M. hyopneumoniae 7448 and J, and M. flocculare was achieved. Many of the identified proteins were differentially represented in M. hyopneumoniae 7448 in comparison to M. hyopneumoniae J and M. flocculare, including potential PEP determinants, such as adhesins, proteases, and redox-balancing proteins, among others. The LC-MS/MS data also provided experimental validation for several genes previously regarded as hypothetical for all analyzed mycoplasmas, including some coding for proteins bearing virulence-related functional domains. The comprehensive proteome profiling of two M. hyopneumoniae strains and M. flocculare provided tens of novel candidates to PEP determinants or virulence factors, beyond those classically described.

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“…In M. hyopneumoniae the adherence process is mainly mediated by receptor-ligand interactions and adhesins like P97 and P146 (Ferreira et al, 2007). Repeat region 1 (RR1), a locus containing tandem repeats (TR) in the gene mhp183 (P97) has been suggested as to be immunogenic and essential for ciliary binding (Wilton et al, 1998;Minion et al, 2000).…”

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confidence: 99%