Developmental and hyperthermia-induced expression of the heat shock proteins HSP60 and HSP70 in tissues of the housefly Musca domestica: an in vitro study

Abstract

The expression pattern of two major chaperones, the heat shock proteins (HSPs) HSP60 and HSP70 was studied in vitro in tissues of the housefly Musca domestica during larval and adult stages of development to identify their immunological relatives and understand their functional significance in normal cellular activities and during thermal stress. Fluorographs of labeled polypeptides and western blots demonstrated that both HSPs are expressed constitutively and heat-induced in all the larval and adult cell types examined. The pattern of whole tissue immunocytochemical staining using anti-HSP60 and anti-HSP70 antibodies corresponded well with the observations from western blots or fluorographs. In developing oocytes, both constitutive and heat inducible expression of HSP60 were regulated in an oocyte stage-specific manner. In unstressed ovaries the expression of these proteins was less pronounced in early stage oocytes (1 st -8 th ) than at later stages (9 th and onward). The heat shock, however, induced both HSP70 and HSP60 to a significantly high level in early stage oocytes (1 st -8 th ) as compared to their respective controls. Our findings indicate the involvement of the HSP60 and HSP70 proteins in the development, growth and differentiation of both somatic and germ line tissues. Furthermore, the enhanced co-expression of HSP70 and HSP60 upon heat shock in various larval and adult cell types suggests the possible role of HSP60 in thermoprotection.

Heteroside-321 Hepatoregeneration Activity In Cases Of Hepatitis Inducted By Local Hyperthermia

“…A lot of experts consider that the most promising in this regard is the local hyperthermia, which is based on a system SelectoTherm (capacitive electro hyperthermia) [7]. The cytotoxic effect of elevated temperature (42 °C or more) is associated with an inflammatory process [8], synthesis of specific heat shock proteins [9], change in membranes conformational state, inhibition of DNA synthesis [10]. This adversely affects not only cancer but also normal cells, particularly their ability to further proliferation (regeneration), because they are particularly sensitive to temperature in S-period of the cell cycle [11].…”

Cloning & sequence identification of Hsp27 gene and expression analysis of the protein on thermal stress in Lucilia cuprina

“…Blowfly, Lucilia cuprina (Diptera), has the characteristic feature to withstand significantly high temperature stress and is widely adapted to variety of environmental stressors (Tiwari et al,1995(Tiwari et al, , 1997Sharma et al, 2006Sharma et al, , 2007. Earlier reports from our laboratory have shown that while the pattern of heat shock response is comparable between Lucilia and Drosophila, Lucilia is much more robust and is able to withstand much higher range of thermal stress (up to 40-42°C) than Drosophila.…”

Effect of temperature and food quality on variability of fitness components and physiology of digestion in the gypsy moth larvae Lymantria dispar L.

“…Pored uloge u odgovoru na toplotni stres, veoma su važne njihove uloge u indukovanoj termotoleranciji i aklimaciji (Bowler, 2005;Sinclair & Roberts, 2005; i u apoptozi (Beere, 2004). S tim u vezi, uočene su velike razlike u ekspresiji Hsp kod različito adaptiranih vrsta (Zatsepina et al, 2001;Evgen'ev et al, 2007;Sorensen & Loeschcke, 2007), kao i razlike u tkivnoj specifičnosti i mehanizmima regulacije (Lakhotia et al, 2002;Gong & Golic, 2006;Sharma et al, 2007;Velu et al, 2008) koje jesu posledice različitih evolucionih putanja vrsta. Na osnovu ćelijske lokalizacije, genske sekvence, molekulske mase i uloge u odgovoru na stresore, Hsp se dele na nekoliko grupa: familija malih hit šok proteina (Hsp22, Hsp23, Hsp26 i Hsp27 kDa) koji se sintetišu nakon delovanja ekstremno visokih temperatura (Evgen'ev et al, 1987;Michaud et al, 2002;Morrow et al, 2006), familija Hsp70, koja uključuje konstitutivni Hscp (kodiran genom za Hsp73) i različite inducibilne Hsp (Hsp72,75,78) (Kregel, 2002) i familija Hsp velikih molekulskih masa (Hsps90, 104/110 kDa) važnih u različitim procesima signalne transdukcije u normalnim i stresnim uslovima (Pratt & Toft, 2003;Chen & Wagner, 2012).…”

“…Ekspresija različitih klasa Hsp kod insekata je različita na različitim (visokim) stresnim temperaturama (Evgenev et al, 1987;Haung et al, 2007;Velu et al, 2008;Karl et al, 2012), u različitim stupnjevima razvića (Sharma et al, 2007;Bowler & Terblanche, 2008;Zhao & Jones, 2012) i u različitim tkivima (Garbuz et al, 2002;Tachibana et al, 2005;Velu et al, 2008;Manjunatha et al, 2010;Li et al, 2012). Važno je napomenuti i različitu ulogu Hsp u oporavku nakon toplotnog stresa (Lakhotia et al, 2002;Gong & Golic, 2006;Sharma et al, 2007;Velu et al, 2008).…”

“…Važno je napomenuti i različitu ulogu Hsp u oporavku nakon toplotnog stresa (Lakhotia et al, 2002;Gong & Golic, 2006;Sharma et al, 2007;Velu et al, 2008).…”