The pgm locus and pigmentation phenotype in Yersinia pestis

Leal-Balbino, Tereza Cristina; Leal, Nilma Cintra; Nascimento, Mirna Gisele Medeiros do; Oliveira, Maria Betânia Melo de; Balbino, Valdir de Queiroz; Almeida, Alzira Maria Paiva de

doi:10.1590/s1415-47572006000100024

Cited by 5 publications

(5 citation statements)

References 19 publications

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“…Pigmentation segment deletion and plasmid deletions in Brazilian strains have been reported (Almeida et al, 1994;Leal et al, 2000;Leal-Balbino et al, 2006). Moreover, Guiyoule et al (1994) found a pigmentation deletion in vitro, which gave a new PFGE type, although they did not suggest that all new PFGE types were due to this deletion.…”

Section: Discussionmentioning

confidence: 99%

Subtyping Brazilian Yersinia pestis strains by pulsed-field gel electrophoresis

Barros

Silveira-Filho²,

Lins³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. We subtyped Brazilian Yersinia pestis strains by pulsed-field gel electrophoresis (PFGE). This was done with 22 Brazilian Y. pestis strains: 17 from an outbreak and 5 from endemic routine surveillance. The strains were divided into 2 groups (I and II), 8 subgroups (A-H) and 19 PFGE profiles or pulsotypes. PFGE did not separate outbreak from non-outbreak strains, as identical pulsotype patterns were found among outbreak strains and strains obtained from surveillance. However, it was able to detect intraspecific genetic diversity among Brazilian strains. This PFGE technique was able to differentiate a homogeneous group of Brazilian Y. pestis strains.

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Section: Discussionmentioning

confidence: 99%

Subtyping Brazilian Yersinia pestis strains by pulsed-field gel electrophoresis

Barros

Silveira-Filho²,

Lins³

et al. 2013

Genet. Mol. Res.

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“…10 However, both studies were performed using the attenuated Y. pestis strain KIM5 which lacks the pgm locus, an important virulence factor. 16 This deletion requires that inoculation of Y. pestis be performed intravenously, a route that ensures rapid dissemination of the bacteria, but one that also bypasses several important processes, in particular those deployed in the skin and the draining lymph node.…”

Section: Discussionmentioning

confidence: 99%

Mus spretus SEG/Pas mice resist virulent Yersinia pestis, under multigenic control

et al. 2010

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Laboratory mice are well known to be highly susceptible to virulent strains of Yersinia pestis in experimental models of bubonic plague. We have found that Mus spretus-derived SEG/Pas (SEG) mice are exceptionally resistant to virulent CO92 and 6/69 wild type strains. Upon subcutaneous injection of 10 2 colony-forming units (CFU), 90% of females and 68% of males survived, compared with only an 8% survival rate for both male and female C57BL/6 mice. Furthermore, half of the SEG mice survived a challenge of up to 10 7 CFU. The time required for mortality was similar between B6 and SEG, suggesting that survival is dependent on early rather than late processes. The analysis of 322 backcross mice identified three significant quantitative trait loci (QTLs) on chromosomes 3, 4 and 6, with dominant SEG protective alleles. Each QTL increased the survival rate by approximately 20%. The three QTLs function additively, thereby accounting for 67% of the difference between the parental phenotypes. Mice heterozygous for the three QTLs were just as resistant as SEG mice to Y. pestis challenge. The SEG strain therefore offers an invaluable opportunity to unravel mechanisms and underlying genetic factors of resistance against Y. pestis infection.

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“…The Y. pestis HPI is found within the pigmentation ( pgm ) locus and comprises approximately one-third of it. The latter is an unstable wide area of the Y. pestis chromosome consisting of an HPI segment linked to a pigmentation segment [ 60 ]. The HPI is distributed widely among the Enterobacteriaceae family members.…”

Section: Yersiniabactinmentioning

confidence: 99%

“…As in other Gram-negative bacteria, Y. pestis produces iron uptake regulation protein (Fur), which is responsible for repressing the transcription of the promoters associated with a Fur binding sequence (FBS) once iron is in excess [ 60 ]. All of the four ybt operons that lie within the HPI possess FBSs promoters and are repressed via Fur approximately 12-fold ( psn ), 8-fold ( irp2-irp1-ybtUTE ), 11-fold ( ybtA ), and 55-fold ( ybtPQXS ) upon the growth with 10 μM iron compared to the growth in deferrated, defined media (PMH/PMH2) without additional iron [ 57 , 58 , 59 , 60 , 61 ]. ybtD , on the contrary, that is encoded outside the HPI and pgm locus is not Fur regulated.…”

Section: Yersiniabactinmentioning

confidence: 99%

Overview of Yersinia pestis Metallophores: Yersiniabactin and Yersinopine

Chaaban

Mohsen²,

Ezzeddine

et al. 2023

Biology

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The pathogenic anaerobic bacteria Yersinia pestis (Y. pestis), which is well known as the plague causative agent, has the ability to escape or inhibit innate immune system responses, which can result in host death even before the activation of adaptive responses. Bites from infected fleas in nature transmit Y. pestis between mammalian hosts causing bubonic plague. It was recognized that a host’s ability to retain iron is essential in fighting invading pathogens. To proliferate during infection, Y. pestis, like most bacteria, has various iron transporters that enable it to acquire iron from its hosts. The siderophore-dependent iron transport system was found to be crucial for the pathogenesis of this bacterium. Siderophores are low-molecular-weight metabolites with a high affinity for Fe3+. These compounds are produced in the surrounding environment to chelate iron. The siderophore secreted by Y. pestis is yersiniabactin (Ybt). Another metallophore produced by this bacterium, yersinopine, is of the opine type and shows similarities with both staphylopine and pseudopaline produced by Staphylococcus aureus and Pseudomonas aeruginosa, respectively. This paper sheds light on the most important aspects of the two Y. pestis metallophores as well as aerobactin a siderophore no longer secreted by this bacterium due to frameshift mutation in its genome.

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The pgm locus and pigmentation phenotype in Yersinia pestis

Cited by 5 publications

References 19 publications

Subtyping Brazilian Yersinia pestis strains by pulsed-field gel electrophoresis

Subtyping Brazilian Yersinia pestis strains by pulsed-field gel electrophoresis

Mus spretus SEG/Pas mice resist virulent Yersinia pestis, under multigenic control

Overview of Yersinia pestis Metallophores: Yersiniabactin and Yersinopine

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