Sequence characterization of coding regions of the myostatin gene (GDF8) from Brazilian Murrah buffaloes (Bubalus bubalis) and comparison with the Bos taurus sequence

Mota, Lígia Souza Lima Silveira da; Curi, Rogério Abdallah; Palmieri, Darío Abel; Borges, Alexandre Secorun; Lopes, Catalina Romero; Barbosa, José Diomedes; Gimenes, Marcos Aparecido

doi:10.1590/s1415-47572006000100016

Cited by 4 publications

(6 citation statements)

References 15 publications

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“…Some SNPs, nt414 (T→C) and/or nt1083 (C→T), in exon 3 of the myostatin genes of both the Qinchuan and Red Angus breeds have also been observed in European cattle breeds, the Nellore breed, and in Brazilian Murrah buffalo (Bubalus bubalis) (Dunner et al, 2003;Mota et al, 2006). This suggests that these 2 sites have a high level of polymorphism, which has previously been generally observed in the myostatin gene of cattle (Grobet et al, 1998).…”

Section: Discussionmentioning

confidence: 52%

Comparative analysis of myostatin gene and promoter sequences of Qinchuan and Red Angus cattle

He¹,

Wu²,

Quan³

et al. 2013

Genet. Mol. Res.

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ABSTRACT.To better understand the function of the myostatin gene and its promoter region in bovine, we amplified and sequenced the myostatin gene and promoter from the blood of Qinchuan and Red Angus cattle by using polymerase chain reaction. The sequences of Qinchuan and Red Angus cattle were compared with those of other cattle breeds available in GenBank. Exon splice sites were confirmed by mRNA sequencing. Compared to the published sequence (GenBank accession No. AF320998), 69 single nucleotide polymorphisms (SNPs) were identified in the Qinchuan myostatin gene, only one of which was an insertion mutation in Qinchuan cattle. There was a 16-bp insertion in the first 705-bp intron in 3 Qinchuan cattle. A total of 7 SNPs were identified in exon 3, in which the mutation occurred in the third base of the codon and was synonymous. On comparing the Qinchuan myostatin gene sequence to that of Red Angus cattle, a total of 50 SNPs were identified in the first and third exons. In addition, there were 18 SNPs identified in the Qinchuan cattle promoter region compared with those of other cattle

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Section: Discussionmentioning

confidence: 52%

Comparative analysis of myostatin gene and promoter sequences of Qinchuan and Red Angus cattle

He¹,

Wu²,

Quan³

et al. 2013

Genet. Mol. Res.

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“…Twelve SNPs were identified in the three exons of the myostatin gene in Brazilian buffaloes (Bubalus bubalis), when compared with the Bos taurus sequences, leading to five amino acid substitutions in the protein sequence (Mota et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Myostatin (GDF8) single nucleotide polymorphisms in Nellore cattle

Grisolia¹,

D’Angelo²,

Porto-Neto³

et al. 2009

Genet. Mol. Res.

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ABSTRACT. The myostatin gene, also known as GDF8 (growth differentiation factor 8), is located on bovine chromosome 2 (BTA2); it has three exons and two introns. Myostatin is specifically expressed during embryonic development and in adult skeletal muscle, functioning as a negative regulatory protein. Several cattle breeds (Piedmontese, Belgian Blue and Blond'Aquitaine, and others) show polymorphisms in this gene; these polymorphisms are directly related to the double muscling phenotype. We looked for polymorphisms in the Nellore cattle myostatin gene and compared them with those known for taurine breeds. Seven regions, covering the three exons of this gene, were amplified by polymerase chain reaction and sequenced, including the untranslated region. DNA from 30 adult Nellore animals was collected; DNA sequencing revealed three, seven and four polymorphisms in exons 1, 2 and 3, respectively. We found previously reported polymorphisms, as well as several new ones; for instance, 37 polymorphisms were found in the untranslated region segment, and in introns 1 and 2 there were one and three polymorphisms, respectively. The high degree of allelic heterogeneity in the myostatin gene could be related to its high mutation rate; it also could be the result of a long history of artificial selection for meat production, which has probably favored such modifications and maintained them in cattle populations. These polymorphisms identified in Nellore cattle could be useful for breeding programs.

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“…Although the amplification protocol of the study of Mota et al was applied [13], the fusion temperature used in this only allowed the correct amplification of exon 3, due to this, this temperature was modified for exons 1 and 2 for achieving the correct amplification Table 1.…”

Section: Resultsmentioning

confidence: 99%

“…An animal of the Holstein breed of the Bos taurus specie was also selected as a control for the amplification of the Myostatin gene. 4 mL of blood was taken directly from the coccygeal vein in an EDTA anticoagulated tube (Vaccutainer Becton Dikiinson, USA), homogenized and kept refrigerated (5 o C) [13] and sent to the University of La Salle, molecular biology laboratory for processing.…”

Section: Population and Sample Collectionmentioning

confidence: 99%

“…DNA was extracted using DNeasy Blood and Tissue Kit (QIAGEN Corporation, USA) following manufacturer instructions, and the obtained DNA was kept at -20ºC until PCR was performed. First, second and third exon of the myostatin gene was amplified using the primers designed for Bos Taurus previously described by [13], with minor modifications. For the amplification the kit GoTaq® Green Master Mix (Promega, Corporation; USA) was used, in a C1000 thermocycler (BIO-RAD, CA, USA, with the following conditions: 4 minutes of denaturalization, and 35 cycles of 1 minute 94°C, 54°C one minute, an extension of 1 minute at 72°C and a final extension of 4 minutes at 72°C [13].…”

Section: Dna Processing and Sequencingmentioning

confidence: 99%

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Sequencing and Analysis of the Myostatin Gene (GDF-8) in Bubalus bubalis Young Animals to Determine the Existence of Possible Mutations Expressed in Double Musculature Phenotype

et al. 2021

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Since the 19th century, the presentation of bovines with disproportionate muscle development have been associated with mutations that inhibit the action of the myostatin gene, it is referred to as double muscle mutation, which is common in some European Bos taurus breeds but it is not reported in buffaloes Bubalus bubalis. This study aims to evaluate if the phenotype observed in 6 young buffaloes with disproportionate muscle development has the same myostatin mutation reported in cattle. DNA was obtained from the blood of the animals of the Murrah breed. First, second and third exon was amplified end point PCR; the fragments were sequenced using capillary electrophoresis. Holstein cattle (Bos taurus) was used As control for normal phenotype. The results obtained from the comparison of the sequence of the myostatin gene show that the observed double-muscled phenotype did not show differences from normal controls. Interspecific variation was demonstrated by comparing exons two and three of the gene, finding 12 variations between the Bos taurus and Bubalus bubalis species in the evaluated fragments. It is necessary to study physiology, and the animals to explain the phenotype observed in buffaloes.

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Sequence characterization of coding regions of the myostatin gene (GDF8) from Brazilian Murrah buffaloes (Bubalus bubalis) and comparison with the Bos taurus sequence

Cited by 4 publications

References 15 publications

Comparative analysis of myostatin gene and promoter sequences of Qinchuan and Red Angus cattle

Comparative analysis of myostatin gene and promoter sequences of Qinchuan and Red Angus cattle

Myostatin (GDF8) single nucleotide polymorphisms in Nellore cattle

Sequencing and Analysis of the Myostatin Gene (GDF-8) in Bubalus bubalis Young Animals to Determine the Existence of Possible Mutations Expressed in Double Musculature Phenotype

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