Comparative analysis of myostatin gene and promoter sequences of Qinchuan and Red Angus cattle

He, Yulong; Wu, You; Quan, Franklin; Liu, Y G; Zhang, Y

doi:10.4238/2013.september.4.6

Cited by 5 publications

(10 citation statements)

References 25 publications

(26 reference statements)

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“…In fact, the alignment of chicken MSTN 5′ regulatory region with mammal species (human, mouse and bovine) revealed a low level of sequence similarity (30% between chicken and human) [74], which was also observed in our analysis (Additional file 2). Similar results were obtained when comparing the 5′ regulatory region of birds with more distantly related animals, such as Xenopus tropicalis and zebrafish ( mstn - 1 and -2 ), indicating sequence variation between such organisms [43]. However, when comparisons were performed with mammals and birds versus fish mstn-1 , a short region of MSTN promoter was found conserved (Fig.…”

Section: Conservation Of Mstn Gene Promoter and 5′ Regulatory Regionsupporting

confidence: 77%

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Myostatin gene promoter: structure, conservation and importance as a target for muscle modulation

Grade

Mantovani

Alvares

2019

J Animal Sci Biotechnol

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Myostatin (MSTN) is one of the key factors regulating myogenesis. Because of its role as a negative regulator of muscle mass deposition, much interest has been given to its protein and, in recent years, several studies have analysed MSTN gene regulation. This review discusses the MSTN gene promoter, focusing on its structure in several animal species, both vertebrate and invertebrate. We report the important binding sites considering their degree of phylogenetic conservation and roles they play in the promoter activity. Finally, we discuss recent studies focusing on MSTN gene regulation via promoter manipulation and the potential applications they have both in medicine and agriculture. Electronic supplementary material The online version of this article (10.1186/s40104-019-0338-5) contains supplementary material, which is available to authorized users.

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Section: Conservation Of Mstn Gene Promoter and 5′ Regulatory Regionsupporting

confidence: 77%

“…Like in porcine and bovine, single nucleotide mutations were already reported in the sheep MSTN promoter sequence [43, 61], as summarized in Table 1.…”

Section: Sheep Mstn Gene Promotermentioning

confidence: 99%

Myostatin gene promoter: structure, conservation and importance as a target for muscle modulation

Grade

Mantovani

Alvares

2019

J Animal Sci Biotechnol

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“…The Ho value lower than He indicated that an inbreeding probability were occured in this population (Nassiry et al, 2009). Polymorphism in promoter region was also identified by He et al (2013) at locus -371 of MSTN promoter gene which has 18 polymorphic SNPs in Qinchuan cattle. The most valuable meat is from longissimus dorsi which is in this research overall average of LDT and BFT were 33.047±5.077 mm and 1.455±0.348 mm, respectively.…”

Section: Polymorphism Of Myostatin Promoter Genementioning

confidence: 80%

Polymorphism of Myostatin (MSTN) Promoter Gene and its Association with Growth and Muscling Traits in Bali Cattle

Khasanah

Gunawan

Priyanto

et al. 2016

Med Pet

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Myostatin (MSTN) gene plays a key role in skletal muscle homeostasis such as inducing muscle athrophy, poliferation of myoblast, increasing ubiquitin-proteasomal, downregulating IGF pathway, and glucolysis. Myostatin gene expression is controled by CpG island located in promoter region. The objectives of this research were to identify polymorphism of MSTN promoter gene and to associate the polymorphism of SNP with growth and muscling traits in Bali cattle. A total of 48 Bali cattle from BPTU-HMT Bali island was screened to identify genetic polymorphisms in MSTN promoter region using sequencing method. The growth and muscling traits were measured at 12 months of age. The muscling traits were evaluated using ultrasound console with linear transducer having frequency 6.5 Hz and scaning we conducted at 130 mm in deep. Analysis of polymorphism was conducted by using PopGen 1.32 software. The association of MSTN with growth and muscling traits were analyzed by using General Linear Model (GLM) procedure. This result showed that a total of 20 polymorphic SNPs (seven SNPs in CpG island) were detected in this region. Although, only 3 SNPs (g.-8078C>T, g.-7996G>C, and g.-7930A>G) had equilibrium condition in Hardy-Weinberg analysis. The association result showed that 2 SNPs (g.-7799T>C and g.-7941C>T) were significantly associated with intramuscular fat percentage (P≤0.05) in Bali cattle. Although the 2 SNPs were nominally significant at nominal P≤0.05 threshold, they were not significant after Bonferroni correction for multiple testing. It could be concluded that MSTN promoter gene was polymorphic in Bali cattle and there were 2 SNPs associated with carcass quality.

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“…Interest for its investigation does not fade. Various allelic variants has been identified, the relationships of single nucleotide substitutions in this gene with productive traits in various animals has been determined (Ye et al, 2007;He et al, 2013;Hope et al, 2013;Tu et al, 2013;Dementeva et al, 2017;Mitrofanova et al, 2017;Rooney et al, 2017). There are studies confirming the associative links of SNPs in the region of the myostatin gene with the growth rate of young chickens and body weight (Dushyanth et al, 2016).…”

Section: Discussionmentioning

confidence: 97%

Efficiency of using SNP markers in the <i>MSTN</i> gene in the selection of the Pushkin breed chickens

Дементьева¹,

Vakhrameev²,

Larkina³

et al. 2020

Vestn. VOGiS

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In the poultry industry, indicators reflecting the growth rate of young stock and the exterior characteristics of chickens are important benchmarks for breeding. Traditional selection based on phenotypic evaluation is characterized by low efficiency with a low character inheritance ratio and is difficult to apply in small groups of animals and birds bred in bioresource collections. The use of molecular genetic markers associated with economically important traits makes it possible to carry out early selection of birds. This entails an increase in the profitability of the poultry industry. Recently, single nucleotide polymorphisms (SNPs) have served as convenient markers for selection purposes. For five generations (P1–P5), an experimental selection of hens of the Pushkin breed was carried out for live weight. It was based on selection for single nucleotide polymorphism rs313744840 in the MSTN gene. As a result, a significant increase in the frequency of allele A in this gene, from 0.11 to 0.50, took place. The association of SNP markers with meat qualities in the experimental group led to changes in the exterior profile of an adult bird at 330 days of age. The individuals with the AA and AG genotypes had the greatest live weight and longest body. As a result of selection, the bird on average became larger due to an increase in the number of heterozygous individuals with long bodies and large chest girths. The depth of the chest and the width of the pelvis increased due to an increase in the frequency of allele A in the experimental population. A tendency towards an increase in these indicators with the substitution of G with A in the genotype was found. Saturation of the population with desirable alleles led to an increase in the average live weight of the chickens. Analysis of the exterior parameters of adult birds showed that this growth is achieved by increasing the depth and volume of the bird body, and not by increasing the length of the limbs. Thus, marker selection carried out for five generations in the experimental population of Pushkin breed chickens to increase body weight has reliably (p < 0.001) changed the exterior profile of adult birds.

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Comparative analysis of myostatin gene and promoter sequences of Qinchuan and Red Angus cattle

Cited by 5 publications

References 25 publications

Myostatin gene promoter: structure, conservation and importance as a target for muscle modulation

Myostatin gene promoter: structure, conservation and importance as a target for muscle modulation

Polymorphism of Myostatin (MSTN) Promoter Gene and its Association with Growth and Muscling Traits in Bali Cattle

Efficiency of using SNP markers in the <i>MSTN</i> gene in the selection of the Pushkin breed chickens

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