Association of MYF5 gene allelic variants with production traits in pigs

Abstract

We studied the phenotypic effects of polymorphisms at the MYF5 gene in a divergent F 2 swine population and found that one polymorphism was due to an insertion and another to a deletion. The genotypes of 359 F2 animals were obtained and the Normal/Normal (NN) and Normal/Insertion (NI) genotypes analyzed to determine associations with phenotypic data for performance, carcass and meat quality traits. Significant differences were observed (p < 0.05) between NN and NI animals for drip (NN = 3.14 ± 1.56; NI = 3.69 ± 2.78%), cooking (NN = 32.26 ± 2.41; NI = 33.21 ± 2.31%) and total loss (NN = 34.16 ± 2.63 and NI = 34.97 ± 2.08%). The Deletion marker was not statistically tested. The results indicate that the allelic variant Insertion is associated with a deleterious effect on meat quality traits and should be monitored in marker assisted selection programs.

Associations of MYF5 gene polymorphisms with meat quality traits in different domestic pig (Sus scrofa) populations

“…Our results showed that two haplotypes, AA/CD and AA/DD, were highly frequent in population 2 and showed significant difference in their effects on drip loss rate (DLR) (p < 0.05) and water holding capacity (WHC) (p < 0.05). This supports the results of Carmo et al (2005), who found that MYF5 gene allelic variants had a significant effect on DLR, cooking properties and total cooking loss in a divergent F2 pig population (n = 359) of Brazilian Piau boars and commercial white females and that an insertion (I) variation in the MYF5 gene is associated with water-holding capacity in the same population. In our work, we also found that there were significant differences in population 2 between the different haplotypes in respect of the biceps femoris meat color value (MCV2, p < 0.05), biceps femoris marbling score (MM2, p < 0.05), longissimus dorsi intramuscular fat percentage (IMF, p < 0.01) and longissimus dorsi moisture content (WM, p < 0.01).…”

“…The MYF5 gene (Ott et al, 1991) is first inducibly expressed in muscle cells during embryonic muscle development, with many discrete regulatory elements being involved in the activation and maintenance of MYF5 gene expression in the various muscle precursor populations (Teboul et al, 2002). The effects of MYF5 gene restriction fragment length polymorphism (RFLP) on carcass traits have been described by various authors (Stratil and Cepica, 1999;te Pas et al, 1999;Cieslak et al, 2002;Urbanski and Kuryl, 2004) and this gene has been considered a candidate gene for meat production and meat quality (te Pas, 2004;Carmo et al, 2005). However, few studies on the effect of MYF5 gene polymorphisms on meat quality have, in fact, been published and before such polymorphisms can be used efficiently in breeding and management decisions studies with different polymorphisms in different populations are required to properly characterize any associations of this gene with economically important traits across pig populations.…”

Lack of an association between a single nucleotide polymorphism in the bovine myogenic determination 1 (MyoD1) gene and meat quality traits in indigenous Chinese cattle breeds

“…Myogenesis, a complex process invoved in muscle fiber formation throughout embryonic development, differentiation and maturation (Pette and Staron, 1997;te Pas et al, , 1999a, is completely controlled by the MyoD gene family. MyoD1 and MYF5 were believed to be candidate genes for meat production and meat quality (te Pas, 2004;Carmo et al, 2005). In previous studies, polymorphism in the MyoD gene famIn previous studies, polymorphism in the MyoD gene family and the association with carcass, meat quality, and reproduction traits in different pig lines and breeds have been reported (te Pas et al, 1999b;Cieslak et al, 2000;Carmo et al, 2005;Wyszynska-Koko et al, 2006;Humpolíček et al, 2007;Verner et al, 2007).…”

“…MyoD1 and MYF5 were believed to be candidate genes for meat production and meat quality (te Pas, 2004;Carmo et al, 2005). In previous studies, polymorphism in the MyoD gene famIn previous studies, polymorphism in the MyoD gene family and the association with carcass, meat quality, and reproduction traits in different pig lines and breeds have been reported (te Pas et al, 1999b;Cieslak et al, 2000;Carmo et al, 2005;Wyszynska-Koko et al, 2006;Humpolíček et al, 2007;Verner et al, 2007). Furthermore, polymorphism in the MyoD gene family and the effects on growth and carcass traits in Korean cattle, Han Woo, have also been reported (Bhuiyan et at., 2009).…”

Association between G316A growth hormone polymorphism and economic traits in pigs

“…The F1 generation consisted of 106 sows and 134 boars (Band et al, 2005;Carmo et al, 2005). Twelve boars from different litters were randomly selected from the 134 F1 boars and mated by natural breeding with 54 F1 sows to produce the F2 generation.…”