Effect of phenobarbital on inducing insecticide tolerance and esterase changes in Aedes aegypti (Diptera: Culicidae)

Sousa-Polezzi, Rita de Cássia; Bicudo, Hermione Elly Melara de Campos

doi:10.1590/s1415-47572004000200024

Cited by 11 publications

(9 citation statements)

References 32 publications

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“…Phenobarbital induction of P450s has been demonstrated across many insect species, including Drosophila melanogaster (Meigen) (Sun et al 2006), Helicoverpa zea (Boddie) (Li et al 2002), Heliothis armigera (Hü bner) (Zhou et al 2010), Blattella germanica (L.) (Brown et al 2003), and Aedes aegypti (L.) (Sousa-Polezzi and Bicudo 2004). Caffeine also has been reported to induce P450s in D. melanogaster (Bhaskara et al 2006, Willoughby et al 2006.…”

Section: Discussionmentioning

confidence: 99%

Transcription and Induction Profiles of Three Novel P450 Genes in Liposcelis bostrychophila (Psocoptera: Liposcelididae)

et al. 2012

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The cDNAs of three novel P450 genes, CYP4CB1, CYP4CC1, and CYP4CD1 (GenBank accessions EU979550, EU979549, and EU979551, respectively), were sequenced and characterized from the psocid Liposcelis bostrychophila Badonnel (Psocoptera: Liposcelididae). CYP4CB1, CYP4CC1, and CYP4CD1 contain open reading frames of 1533, 1512, and 1536 nucleotides that encode 511,504, and 512 amino acid residues, respectively. The putative proteins of CYP4CB1, CYP4CC1, and CYP4CD1 show predicted molecular mass of 59.65, 58.87, and 59.71 kDa, with a theoretical isoelectric point of 6.59, 8.80, and 8.84, respectively. The N-terminal transmembrane domain was only found in CYP4CB1 suggested it is a typical microsomal P450. Phylogenetic analysis showed a close relationship of CYP4CB1, CYP4CC1, and CYP4CD1 with CYP4AW1, CYP4L4, and CYP4E2. Quantitative real-time reverse transcriptase-polymerase chain reaction indicated that these three genes were expressed at all tested developmental stages. In addition, the highest expression occurred in the adult stage, which suggested that these three P450 genes may play important roles in adulthood. The transcripts of CYP4CB1 and CYP4CC1 in adult psocids could be induced to the highest level at 36 and 24 h after the exposure to deltamethrin and paraoxon-methyl (50% lethal concentration [LC50]), respectively, whereas CYP4CD1 remained unchanged. CYP4CD1 transcripts, however, increased rapidly at 8 h after aldicarb (LC50) induction and reached the peak at 36 h. The induction profiles of the three P450 genes suggested that CYP4CB1 and CYP4CC1 are possibly associated with deltamethrin and paraoxonmethyl metabolism in psocids, whereas CYP4CD1 is probably involved in aldicarb metabolism. However, our assumption needs to be further verified by recombinant protein expression of these proteins as well as RNA interference of these genes.

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Section: Discussionmentioning

confidence: 99%

Transcription and Induction Profiles of Three Novel P450 Genes in Liposcelis bostrychophila (Psocoptera: Liposcelididae)

et al. 2012

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“…Phenobarbital (PB) treatment has a variety of effects on mammals: (1) reduces frequency of epileptic seizures (Baumann, 2001; Hernandez et al ., 2002; Kwan & Brodie, 2004; Zhong et al ., 2004); (2) induces resistance/tolerance to insecticides in rodents, cows and humans (Fries et al ., 1971; Zavon, 1971; Vitarius et al ., 1995); and (3) causes liver cancer (Henry, 1997; Ferko et al ., 2003; Calvisi et al ., 2004; Hokaiwado et al ., 2004). PB induction of P450s has also been demonstrated across insect species, including Drosophila hydei (Danielson et al ., 1997), Helicoverpa zea (Li et al ., 2002), Blatella germanica (Brown et al ., 2003), D. melanogaster (Amichot et al ., 1994), Aedes aegypti (Sousa‐Polezzi & Bicudo, 2004) and across multiple P450 families (Danielson et al ., 1998). In insects, the best‐studied P450 response element for plant allelochemicals has been the xenobiotic response element to xanthotoxin (XRE‐xan) (Petersen et al ., 2003; McDonnell et al ., 2004) and this element has been identified as an important component in the induction of the Cyp6b1v3 gene in the black swallowtail butterfly ( Papilio polyxenes ).…”

Section: Introductionmentioning

confidence: 99%

Genome‐wide analysis of phenobarbital‐inducible genes in Drosophila melanogaster

Sun

Margam

Sun

et al. 2006

Insect Molecular Biology

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An oligoarray analysis was conducted to determine the differential expression of genes due to phenobarbital exposure in Drosophila melanogaster (w(1118) strain) third instar larvae. Seventeen genes were observed to be induced with increased expression by a statistical analysis of microarrays approach with a q < or = 0.05. At q < or = 0.12, four more genes (Cyp12d1, DmGstd4, and two genes with unknown function) were found to be up-regulated, and 11 genes with unknown function were found to be down-regulated. Fifteen of these genes, Cyp4d14, Cyp6a2, Cyp6a8, Cyp12d1, Cyp6d5, Cyp6w1, CG2065, DmGstd6, DmGstd7, Amy-p/Amy-d, Ugt86Dd, GC5724, Jheh1, Jheh2 and CG11893, were verified using quantitative real time polymerase chain reaction. Some of these genes have been shown to be over-transcribed in metabolically DDT-resistant Drosophila strains.

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“…O estudo da estrutura genética da população é essencial para o entendimento da dinâmica das populações do A. aegypti e para a análise de fatores responsáveis pela resistência e adaptação ecológica. Vários estudos têm investigado a variabilidade genética de populações de culicídeos, utilizando marcadores moleculares tais como isoenzimas (Laranja et al 2003, Souza-Polezzi & Bicudo 2004, DNA mitocondrial (Bosio et al 2005), RAPD (Polimorfismo de DNA Amplificado ao Acaso) (Paduan et al 2006) e microssatélites (Chambers et al 2007).…”

Section: Palavras-chave: Ve Tor Da Dengue Genética De Populações Reunclassified

Variabilidade Genética em Populações de Aedes aegypti (L.) (Diptera: Culicidae) Utilizando Marcadores de RAPD

Hiragi¹,

Simões²,

Martins³

et al. 2009

Neotrop. entomol.

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-Aedes aegypti (L.) is an important vector of diseases such as the yellow fever and dengue, present in tropical and subtropical regions. The objective of this study was to analyze the genetic variability of different A. aegypti populations using RAPD (Random Amplified Polymorphic DNA) markers as a basic study to support the use of biocontrol strategies. DNA of ten collected larvae from three different populations were analyzed using ten RAPD primers. The results indicated the existence of genetic variability inter and intrapopulation. This was confirmed by a dendrogram that grouped the populations in two main clusters with a genetic similarity of 24%. In one of these clusters, it was possible to distinguish two populations that showed 50% similarity. The molecular variance analysis indicated that the interpopulation genetic diversity (55,01%) was higher than the intrapopulation genetic diversity (44,99%). A high genetic polymorphism (H t = 0.2656) and high levels of genetic differentiation between populations (G st = 0.3689) were found. The adopted DNA extraction protocol proved to be efficient regardless the insect development stage used, avoiding the addition of reagents or additional stages of processing. Future experiments can be performed to confirm if the detected variability is related to the resistance characteristics of each population to a determined pesticide.KEY WORDS: Dengue vector, population genetic, resistance RESUMO -Aedes aegypti (L.) é vetor de importantes doenças como a febre amarela e a dengue, presentes em regiões tropicais e subtropicais. Para o sucesso no seu controle biológico é importante conhecer a estrutura genética e os mecanismos que resultaram na diversidade das populações. O objetivo deste estudo foi analisar a variabilidade genética de diferentes populações de A. aegypti utilizando marcadores de RAPD (Polimorfismo de DNA amplificado ao acaso). DNA de dez larvas coletadas a partir de três populações de diferentes localidades foi analisado usando dez iniciadores de RAPD. Os resultados indicaram a existência de variabilidade genética inter e intrapopulacional. Isso foi confirmado por um dendrograma que agrupou as populações em dois blocos principais com similaridade genética de 24%. Em um desses agrupamentos foi possível distinguir duas populações que apresentaram grau de similaridade de 50%. A diversidade genética entre as populações (55,01%) foi mais elevada que a diversidade genética dentro das populações (44,99%) aplicando-se análise por AMOVA. Altos níveis de polimorfismo genético (H t = 0.2656) e de diferenciação genética entre as populações (G st = 0.3689) foram observados. Além disso, o protocolo de extração de DNA adotado mostrou-se eficiente para a análise do inseto independente do seu estágio de desenvolvimento, evitando-se o acréscimo de reagentes ou etapas adicionais de processamento. Futuros experimentos poderão ser realizados para confirmar se a variabilidade observada pode estar ligada às características de resistência de cada população a um determinado pest...

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Effect of phenobarbital on inducing insecticide tolerance and esterase changes in Aedes aegypti (Diptera: Culicidae)

Cited by 11 publications

References 32 publications

Transcription and Induction Profiles of Three Novel P450 Genes in Liposcelis bostrychophila (Psocoptera: Liposcelididae)

Transcription and Induction Profiles of Three Novel P450 Genes in Liposcelis bostrychophila (Psocoptera: Liposcelididae)

Genome‐wide analysis of phenobarbital‐inducible genes in Drosophila melanogaster

Variabilidade Genética em Populações de Aedes aegypti (L.) (Diptera: Culicidae) Utilizando Marcadores de RAPD

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