A rapid alternative technique for obtaining silver-positive patterns in chromosomes

Kavalco, Karine Frehner; Pazza, Rubens

doi:10.1590/s1415-47572004000200012

Cited by 25 publications

(13 citation statements)

References 16 publications

(17 reference statements)

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“…DAPI/CMA 3 staining was carried out as described by Schweizer (1976) with modifications by Guerra and Souza (2002). Ag-NOR staining was performed according Multiple NORs and karyotype variation in S. xanthotricha to Howell and Black (1980) with modifications by Kavalco and Pazza (2004), using 25% AgNO 3 , 1% gelatin, and 0.25% formic acid. A minimum of 10 metaphases per specimen were analyzed.…”

Section: Methodsmentioning

confidence: 99%

Occurrence of multiple nucleolus organizer regions and intraspecific karyotype variation in Scaptotrigona xanthotricha Moure (Hymenoptera, Meliponini)

Duarte¹,

Martins²,

Waldschmidt³

et al. 2009

Genet. Mol. Res.

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ABSTRACT. Scaptotrigona xanthotricha has a wide geographic distribution in the Brazilian Atlantic rainforest. One population from southeast and two from northeast Brazil were analyzed and were found to have chromosome polymorphisms. Although the chromosome number 2n = 34 is conserved in this species, karyotypic analysis revealed clear differences between the three populations. Congruent and ubiquitous multiple nucleolus organizer regions, heterochromatin and CMA 3 -positive blocks were found. The variations suggest that this species is in a process of genetic differentiation. This differentiation process might have been enhanced by restricted nesting preferences, combined with recent extensive fragmentation of the Atlantic rainforest, which limits gene flow between populations.

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Section: Methodsmentioning

confidence: 99%

Occurrence of multiple nucleolus organizer regions and intraspecific karyotype variation in Scaptotrigona xanthotricha Moure (Hymenoptera, Meliponini)

Duarte¹,

Martins²,

Waldschmidt³

et al. 2009

Genet. Mol. Res.

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“…Fluorochrome staining (DAPI/CMA 3 ) was performed following Schweizer (1980). The nucleolar organizing regions (NORs) were identified by silver nitrate staining (Howell and Black 1980) with a few modifications (Kavalco and Pazza 2004). We analyzed 20 metaphases per individual, and the bestquality ones were photographed under an Olympus CX-41 microscope.…”

Section: Chromosome Preparation and Bandingmentioning

confidence: 99%

Investigation of Partamona helleri (Apidae, Meliponini) B chromosome origin. An approach by microdissection and whole chromosome painting

et al. 2012

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-The stingless bee Partamona helleri in southeast Brazil shows the regular chromosome number 2n= 34 and a variable number of up to four minute B1 or B2 chromosomes. Previous cytogenetic analyses have indicated morphological similarities between the B1 chromosome and chromosome segments in the regular karyotype. In this study, microdissection and chromosome painting were employed along with C banding, NOR banding, and base-specific fluorochrome staining to investigate the origin of the B1 chromosome in P. helleri. B1-generated probe hybridized exclusively to B1 chromosomes. This result suggests an independent origin from the regular karyotype or, alternatively, that the B chromosome may have suffered substantial genetic alterations along its independent evolution. The absence of higher dosages of these small B chromosomes in this population of P. helleri may be related to the existence of either a genetic or cytogenetic constraint in the establishment of such high numbered karyotypes.Hymenoptera / B chromosome probe / stingless bee

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“…The cell suspension was centrifuged at 1000 rpm for 6 min and the cells fixed in methanol:acetic acid (3:1). Freshly prepared slides were submitted to silver nitrate (Kavalco and Pazza, 2004) and acridine orange staining (Abrams et al, 1993), and FISH (Viegas-Péquignot, 1992) using as probe a recombinant plasmid (HM123) containing a fragment of Xenopus laevis 28S rDNA, biotinlabeled by nick-translation reaction. The slides with the different staining were analyzed in a light and fluorescence microscope (Axioskop 2 -Zeiss) fitted with an image analyzer.…”

Section: Cell Suspension and Staining Proceduresmentioning

confidence: 99%

Nucleolar behavior during meiosis in four species of phyllostomid bats (Chiroptera, Mammalia)

Beguelini¹,

Marchesin²,

Azeredo-Oliveira³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. We analyzed the behavior of the nucleolus, nucleolar structures and nucleolus organizer regions (NORs) during meiotic division in four species of phyllostomid bats that have different numbers and locations of NORs. Nucleoli began disassembly at leptotene, and the subcomponents released from the nucleolus were dispersed in the nucleoplasm, associated with perichromosomal regions, or they remained associated with NORs throughout division. In Phyllostomus discolor, a delay in nucleolus disassembly was observed; it disassembled by the end of pachytene. The RNA complexes identified by acridine orange staining were observed dispersed in the nucleoplasm and associated with perichromosomal regions. FISH with rDNA probe revealed the number of NORs of the species: one NOR in Carollia per- Nucleolar behavior in meiosis of bats spicillata, one pair in Platyrrhinus lineatus and P. discolor, and three pairs in Artibeus lituratus. During pachytene, there was a temporary dissociation of the homologous NORs, which returned to pairing at diplotene. The variation in the number (from one to three pairs) and location of NORs (in sex or autosomal chromosomes, at terminal or interstitial regions) did not seem to interfere with the nucleolar behavior of the different species because no variation in nucleolar behavior that could be correlated with the variation in the number and chromosomal location of NORs was detected.

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A rapid alternative technique for obtaining silver-positive patterns in chromosomes

Cited by 25 publications

References 16 publications

Occurrence of multiple nucleolus organizer regions and intraspecific karyotype variation in Scaptotrigona xanthotricha Moure (Hymenoptera, Meliponini)

Occurrence of multiple nucleolus organizer regions and intraspecific karyotype variation in Scaptotrigona xanthotricha Moure (Hymenoptera, Meliponini)

Investigation of Partamona helleri (Apidae, Meliponini) B chromosome origin. An approach by microdissection and whole chromosome painting

Nucleolar behavior during meiosis in four species of phyllostomid bats (Chiroptera, Mammalia)

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