Occurrence of multiple nucleolus organizer regions and intraspecific karyotype variation in Scaptotrigona xanthotricha Moure (Hymenoptera, Meliponini)

Duarte, Olívia Maria Pereira; Martins, C.; Waldschmidt, Ana Maria; Costa, Marco A. Dalla

doi:10.4238/vol8-3gmr598

Cited by 21 publications

(10 citation statements)

References 23 publications

(27 reference statements)

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“…Along with the increase in the number of cytogenetically characterized Partamona species, the pattern of heterochromatin composition has been shown to be constant for members of the tribe Meliponini. Most of the observed heterochromatin found is DAPI-positive, with only a few small CMA 3 -positive blocks associated with nucleolus organizer regions (NORs) in some species [Brito et al, 1997;Brito, 1998;Maffei et al, 2001;Rocha et al, 2003Rocha et al, , 2007 , 2008;Duarte et al, 2009;Martins et al, 2009;Barth et al, 2011;Lopes et al, 2011]. The correlation between positive C-banding, bright CMA 3 bands, and NOR markers has been previously reported for the genus Partamona [Brito et al, 1997[Brito et al, , 2005Brito, 1998], as well as for other species of the tribe Meliponini [Maffei et al, 2001;Rocha et al, 2002Rocha et al, , 2007Duarte et al, 2009;Lopes et al, 2011] and for other organisms such as wasps [Menezes et al, 2011], Hemiptera [Criniti et al, 2009], mollusks [Petrović et al, 2009], and fish [Swarça et al, 2003].…”

Section: Resultsmentioning

confidence: 99%

Different Levels of Chromatin Condensation in Partamona chapadicola and Partamona nhambiquara (Hymenoptera, Apidae)

Lopes

Travenzoli

Fernandes

et al. 2020

Cytogenet Genome Res

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Studies in several organisms have contributed to the understanding of heterochromatin and its biological importance. In bees of the tribe Meliponini, the presence of chromosomes with totally heterochromatic arms has been attributed to the mechanism of karyotype evolution in which this group accumulated heterochromatin to maintain telomere stability after centric fission events. In the present study, the use of classical and molecular cytogenetic techniques as well as automated image analysis software for the description of the karyotypes of Partamonachapadicola and P. nhambiquara bee species revealed variability in the compaction and patterns of chromatin structure. Although both species have the same chromosome number as other species in the genus Partamona (2n = 34), C-banding and image analyses indicated the existence of chromosomes with 3 regions of different staining intensities, suggesting a chromatin structure with distinct patterns and characteristics. Repetitive DNA probes hybridized only in the euchromatic regions, whereas the regions with intermediate staining intensity did not show any hybridization signals. This suggests that these regions present features more similar to heterochromatin. Evidence of the existence of a chromatin class with intermediate condensation compared to euchromatin and heterochromatin indicates a potential mechanism for heterochromatin amplification and demonstrates the need for further studies on this topic. This previously unrecognized class of chromatin should be taken into account in the study of all Meliponini chromosomes.

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Section: Resultsmentioning

confidence: 99%

Different Levels of Chromatin Condensation in Partamona chapadicola and Partamona nhambiquara (Hymenoptera, Apidae)

Lopes

Travenzoli

Fernandes

et al. 2020

Cytogenet Genome Res

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“…two Entedon Dalman, 1820 (Entedoninae) and several Pnigalio Schrank, 1802 (Eulophinae) species (Bernardo et al 2008, Bolsheva et al 2012, Gebiola et al 2012). On the other hand, occurrence of multiple telomeric CMA 3 -positive bands found in the karyotype of Trichospilus diatraeae is apparently derived and quite unusual for parasitoids, but is known for aculeate wasps (Menezes et al 2011) and bees (Duarte et al 2009). In the latter study, most CMA 3 -positive bands are attributed to NORs, and this is probably true for Trichospilus diatraeae as well.…”

Section: Discussionmentioning

confidence: 99%

A cytogenetic study of three parasitic wasp species (Hymenoptera, Chalcidoidea, Eulophidae, Trichogrammatidae) from Brazil using chromosome morphometrics and base-specific fluorochrome staining

Gokhman¹,

Pereira²,

Costa³

2017

CCG

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Chromosomes of three chalcid wasp species from Brazil, Palmistichus elaeisis Delvare et LaSalle, 1993, Trichospilus diatraeae Cherian et Margabandhu, 1942 (both belonging to the family Eulophidae) and Trichogramma pretiosum Riley, 1879 (Trichogrammatidae), were studied using chromosome morphometrics and base-specific fluorochrome staining. The present study confirmed that these species respectively have 2n = 12, 14 and 10. Chromomycin A3 / 4’, 6-diamidino-2-phenylindole (CMA3/DAPI) staining revealed a single CMA3-positive and DAPI-negative band within haploid karyotypes of both Palmistichus elaeisis and Trichogramma pretiosum. This CG-rich band clearly corresponds to the nucleolus organizing region (NOR). Moreover, analogous multiple telomeric bands found on all chromosomes of Trichospilus diatraeae may also represent NORs. Certain features of karyotype evolution of the phylogenetic lineage comprising both Eulophidae and Trichogrammatidae are discussed. The results obtained during the present study demonstrate the importance of chromosome research on tropical parasitoids that remain poorly known in this respect.

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“…Further confirmation should be obtained by specific techniques such as Ag-NOR or in situ hybridization . however, good results with Ag-NOR in bees are restricted to only few species (Maffei et al 2001, Duarte et al 2009) and in situ hybridization is not yet widely used in this group.…”

Section: Chromosomal Organizationmentioning

confidence: 99%

Evidence of separate karyotype evolutionary pathway in Euglossa orchid bees by cytogenetic analyses

Fernandes

Werneck

Pompolo

et al. 2013

An. Acad. Bras. Ciênc.

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Euglossini are solitary bees considered important pollinators of many orchid species. Information regarding chromosome organization is available for only a small number of species in this group. In the present work, the species Euglossa townsendi and E. carolina were analyzed by cytogenetic techniques to collect information that may aid the understanding of their evolution and chromosomal organization. The chromosome number found was n = 21 for males and 2n = 42 for females in the two species. The distribution and amount of heterochromatin regions differed in the two species analyzed, where they were classified as "high" or "low" heterochromatin content, similarly to what has already been performed in social bee species of the genus Melipona. Banding patterns found in this study suggest that other mechanisms may have occurred in the karyotype evolution of this group, unlike those suggested for social bees and ants. Karyotype evolution of solitary bees appears to have occurred as an event separate from other hymenopterans and did not involve chromosome fissions and heterochromatin amplification.

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Occurrence of multiple nucleolus organizer regions and intraspecific karyotype variation in Scaptotrigona xanthotricha Moure (Hymenoptera, Meliponini)

Cited by 21 publications

References 23 publications

Different Levels of Chromatin Condensation in <b><i>Partamona chapadicola</i></b> and <b><i>Partamona nhambiquara</i></b> (Hymenoptera, Apidae)

Different Levels of Chromatin Condensation in <b><i>Partamona chapadicola</i></b> and <b><i>Partamona nhambiquara</i></b> (Hymenoptera, Apidae)

A cytogenetic study of three parasitic wasp species (Hymenoptera, Chalcidoidea, Eulophidae, Trichogrammatidae) from Brazil using chromosome morphometrics and base-specific fluorochrome staining

Evidence of separate karyotype evolutionary pathway in Euglossa orchid bees by cytogenetic analyses

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