Enzymatic variability in Aedes aegypti (Diptera: Culicidae) populations from Manaus-AM, Brazil

Abstract

Eighteen enzymatic loci were analysed in Aedes aegypti populations from four neighbourhoods in the city of Manaus. The analyses showed that the Downtown population was the most polymorphic (p = 55.6%) with higher observed and expected mean heterozygosities (H o = 0.152 ± 0.052; H e = 0.174 ± 0.052, respectively). The least variability was detected in the Coroado and Cidade Nova populations, both with polymorphism of 44.4%. The latter population presented the least observed heterozygosity (H o = 0.109 ± 0.037). Wright's F statistics showed that the mean value of F is was higher than that of F st (F is = 0.164 > F st = 0.048), and from analysis of molecular variance (AMOVA) it was found that 95.12% of the variability is found within populations indicating a certain intra-population differentiation possibly of the microgeographic structure resulting from some barrier in the random coupling. Although the four populations were similar genetically (D = 0.003 to 0.016), the 4.88% differentiation was significant.

Genetic lineages in the yellow fever mosquito Aedes (Stegomyia) aegypti (Diptera: Culicidae) from Peru

“…aegypti populations. Generally speaking, high genetic variability has been found in this species and the variability pattern suggests there are genetically differentiated subpopulations among the many locations analyzed (Yan et al 1999, Gorrochotegui-Escalante et al 2000, de Souza et al 2000, Fraga et al 2003.…”

“…Thus, viral susceptibility, vectorial competence, resistance to insecticides and ecological adaptations can be different among the Peruvian populations of Ae. aegypti as showed in other populations (Lourenço-de-Oliveira et al 2002, Fraga et al 2003. Therefore, knowledge of genetic variation of the vector populations related to geographical and/or historical factors can be highly informative when developing effective control strategies.…”

Genetic Diversity in Dengue Mosquito, Aedes aegypti (Diptera: Culicidae) from Amazon Region: Comparative Analysis with Isozymes and RAPD Loci

“…In Amazonas, Ae. aegypti was detected in 1996 in the city of Manaus, and today high population rates of this mosquito have been recorded, indicating the magnitude of the problem in the region [4,5]. Unfortunately, the situation has worsened in recent years, especially due to the increasing number of cases of dengue hemorrhagic fever.…”

“…The epidemiological importance and the ease of laboratory manipulation of Ae. aegypti have facilitated studies on its population genetics and molecular biology [5][6][7][8][9][10][11][12][13]. Many markers have been used in studies on genetic variability and differentiation, in an attempt to quantify and explain this variability, in terms of its origin, maintenance, and importance to evolution.…”

Variabilidade Genética em Populações de Aedes aegypti (L.) (Diptera: Culicidae) Utilizando Marcadores de RAPD

“…Isso também foi observado em duas amostras de A. aegypti coletadas durante a epidemia de 1997 em Santiago de Cuba, e que foram analisadas pela técnica de RAPD-PCR (Bisset et al 2005). A partir da análise de 18 loci isoenzimáticos também foi constatado, em populações desse culicídeo de quatro vizinhanças da cidade de Manaus, AM, que apesar de elas de serem semelhantes geneticamente (D = 0.003 a 0.016), a diferenciação de 4,88% entre as populações foi significante (Fraga et al 2003). (Apostol et al 1996).…”