Genetic relationships among Arachis species based on AFLP

Abstract

Amplified Fragment Length Polymorphism (AFLP) was used to establish the genetic relationships among 20 species from seven of the nine sections of genus Arachis. The level of polymorphism among nine accessions of the cultivated peanut, A. hypogaea L., was also evaluated. Three combinations of primers were used to amplify the AFLPs. The fragments were separated in 6% denaturing acrylamide gels. A total of 408 fragments were analyzed. An average of 135.3 fragments per primer combination were scored, and the largest number of fragments was 169 using primer combination Eco RI -ACC / Mse I -CTG, while the lowest was 108, with Eco RI -ACT / Mse I -CTT. In general, the genetic relationships established using AFLPs agreed with the classification established using morphology and crossability data. The results indicated that AFLPs are good markers for establishing the relationships among Arachis species. The polymorphism detected in A. hypogaea by this method was higher than the one found with other markers, like RAPDs and RFLPs. However, our data suggest that the polymorphism detected be using AFLP with only three primer combinations is still too low to be used for any kind of genetic study in this species.

Systematics of Bromelioideae (Bromeliaceae)—Evidence from Molecular and Anatomical Studies

“…Relationships between and within closely related genera were also examined with the AFLP method (Vos et al 1995) that is increasingly applied to assess genetic similarity at the interspecific level (e.g., Aggarwal et al 1999;Mace et al 1999;Gimenes et al 2002;Beardsley et al 2003;Després et al 2003). Within Bromelioideae, our special interest is in Aechmea and related genera and in the smaller genera forming the southwestern limit of the family distribution (Ochagavia, Fascicularia, and Greigia).…”

SSR analysis of cultivated groundnut (Arachis hypogaea L.) germplasm resistant to rust and late leaf spot diseases

“…A variety of molecular markers have been used to characterize the genetic diversity in groundnut, e.g. RFLPs (Halward et al 1991), RAPDs (Dwivedi et al 2001;Subramanian et al 2000), and AFLPs Prakash 1997, 2001;Gimenes et al 2002). All these studies have reported low levels of polymorphism within the cultivated gene pool.…”

EST sequencing and SSR marker development from cultivated peanut ( Arachis hypogaea L.)

“…Microsatellites (SSRs) as DNA markers are highly polymorphic, highly abundant, show co-dominant inheritance, analytically simple and readily transferable between different plant species (He et al 2003). Previous studies showed that SSR markers could detect more polymorphism in peanut than other molecular markers like RFLPs (Gimenes et al 2007), AFLPs (He and Prakash, 2001;Gimenes et al 2002) and RAPDs (Dwivedi et al 2001;Subramanian et al 2000).…”