Acid phosphatase polymorphism within and among populations of Cauliflower (Brassica oleracea var botrytis)

Abstract

Eighty-one lines of cauliflower (Brassica oleracea var. botrytis) from 12 populations used to produce commercial hybrids in Brazil were screened for polymorphism in the acid phosphatase system, in order to evaluate the usefulness of this marker for the determination of the parental contamination level in hybrid seeds. Little polymorphism was detected in the examined lines, but the system appeared to be very useful for hybrid identification, since the only condition required was polymorphism between the two parental lines. If the analyzed lines were used for hybrid production, 8.4% and 12.3% of the possible crosses would result in hybrids which can be positively identified using the APS-1 and B 1 loci, respectively. If only one plant of each homozygous type (SS or FF) was analyzed in each population, 41% and 50% of the possible crosses would result in hybrids which can be positively identified using the APS-1 and B 1 loci, respectively.

Systematics and taxonomic delimitation of vegetable, grain and weed amaranths: a morphological and biochemical approach

“…The difference in banding pattern expressed between or within the varieties is very helpful in microclassification. It was very useful for differentiation of varieties of muskmelon (Kato et al 1978), screening for polymorphism of Cauliflower lines to produce commercial hybrids (Marino et al 2002). Computed dendrograms on morphology and dendrogram computed from cumulative data on three parameters (morphology, isozyme polymorphism of acid phosphatase, and phenolic chromatogram) were almost super imposable as far as grouping of species/ morphotypes/accessions are concerned and were very much supportive of the previous findings.…”