The cytogenetics and evolution of forage legumes from Rio Grande do Sul: a review

Schifino-Wittmann, María Teresa

doi:10.1590/s1415-47572000000400044

Cited by 11 publications

(4 citation statements)

References 33 publications

(30 reference statements)

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“…The study investigated Citrus callus and immature seeds. In addition to the method of applying the antimitotic, the use of high concentrations of colchicine or inappropriate exposure times for particular tissues may cause the death of cells and plants because colchicine tolerance varies according to the species (SCHIFINO-WITTMANN, 2000).…”

Section: Resultsmentioning

confidence: 99%

The effects of colchicine on banana stem apex

Rodrigues

Soares

Brant

et al. 2013

RCA

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The cultivation of traditional varieties of banana in Brazil has suffered from major pests and diseases. The breeding of new strains of banana based on the duplication of chromosomes via treatment with antimitotic agents has been proposed as a way of introducing disease resistance into the hybrids produced by banana breeding programs. The aim of this study was to evaluate the action of colchicine in the apex of diploid banana using an antimitotic at different concentrations and exposure times. Apexes of diploid banana 1318-01 were used as plant material. Colchicine was used at concentrations of 0 (control), 2.5, 5.0, 7.5, 10.0 and 12.5 mM under agitation (20 rpm) for periods of 24 and 48 h. We assessed the survival rate of plants subjected to colchicine. The highest survival rate of the explants (91.7%) in 2.5 mM colchicine was found for 24 h of exposure to the antimitotic, and a survival rate of 50% was found at a concentration of 7.5 mM colchicine for 48 h of exposure. The study showed that the survival of the explants was relatively high after the exposure of diploid 1318-01 to colchicine for a period of 24 h. RESUMO: O plantio de variedades de bananeira tradicionais no Brasil tem sido um fator de vulnerabilidade às principais pragas e doenças que afetam a cultura. Diante disso, estratégias alternativas de melhoramento genético, fundamentadas na duplicação de cromossomos induzida por tratamentos com agente antimitótico, têm sido propostas como forma de introduzir resistência a doenças nos híbridos gerados pelos programas de melhoramento genético da bananeira. O objetivo deste trabalho foi verificar a ação da colchicina em ápices caulinares de bananeira diplóide, utilizando-se diferentes concentrações e tempos de exposição ao antimitótico. Como material vegetal, foram utilizados ápices caulinares de bananeira do diplóide 1318-01. A colchicina foi utilizada nas concentrações de zero (tratamento controle); 2,5; 5,0; 7,5; 10,0; 12,5 mM, em solução, sob agitação (20 rpm), por períodos de 24 e 48 h. Foi avaliada a taxa de sobrevivência de plantas submetidas à colchicina. Verificou-se maior sobrevivência dos explantes (91,7%) em 2,5 mM de colchicina por 24 horas de exposição ao antimitótico e 50% na concentração de 7,5 mM de colchicina por 48 horas de exposição. Conclui-se que a exposição do diplóide 1318-01 à colchicina por um período de 24 h possibilita maior taxa de sobrevivência dos explantes.

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Section: Resultsmentioning

confidence: 99%

The effects of colchicine on banana stem apex

Rodrigues

Soares

Brant

et al. 2013

RCA

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“…Further, they also affect evolution and taxonomic investigations (SCHIFINO-WITTMANN, 2000). This study reports a detailed study on microsporogenesis of P. serrato-digitata species.…”

Section: Cerca De 90% Das Espécies Do Gêneromentioning

confidence: 99%

Chromosome stickiness during meiotic behavior analysis of Passiflora serrato-digitata L. (PassifloraCEAE)

et al. 2011

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“…Zohary and Heller (1984) considered T. argentinense and T. polymorphum as synonyms. The other species, T. riograndense is native to southern Brazil (Schifino-Wittmann, 1985;Schifino-Wittmann, 2000) and northern Uruguay (Izaguirre and Beyhaut, 1998). All these Campos region's native populations have evolved into adapted ecotypes through long exposure to environmental stresses, grazing and by competing with the native C 3 and C 4 grasses which have dominated the Campos vegetation over a long period of time.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and DNA content of three South American and three Eurasiatic Trifolium species

et al. 2007

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Six species of Trifolium (T. polymorphum Poir., T. riograndense Burkart, T. argentinense Speg., T. medium L., T. pratense L. and T. repens L.) were analyzed using inter-simple sequence repeats (ISSR) markers. Six selected primers generated 186 polymerase chain reaction (PCR) products exploring 112 loci in 34 genotypes analyzed with molecular sizes ranging from 200 to 1300 bp. These primers were able to discriminate among and within species, with the PCR products being on average 41.6% species-specific and 59.9% polymorphic at the within species level. Nuclear DNA content was determined by flow cytometry and revealed variation among species. The 1Cx genome size values were calculated and were found to range from 0.46 pg (T. pratense) to 0.96 pg (T. polymorphum). Genome size values of South American species were higher than those of Eurasiatic origin. The analyses of the molecular data grouped the six species in agreement with their geographical origin and clearly differentiate T. polymorphum from T. argentinense. The Eurasiatic group showed the highest average of species-specific bands (45.3%) and the South American group exhibited the highest amount of total bands (59.7). The highest level of intra-species polymorphisms was detected in T. argentinense (92.9%), followed by T. medium (89.5%).

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The cytogenetics and evolution of forage legumes from Rio Grande do Sul: a review

Cited by 11 publications

References 33 publications

The effects of colchicine on banana stem apex

The effects of colchicine on banana stem apex

Chromosome stickiness during meiotic behavior analysis of Passiflora serrato-digitata L. (PassifloraCEAE)

Genetic diversity and DNA content of three South American and three Eurasiatic Trifolium species

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