Genetic variability among and within races of Heterodera glycines Ichinohe assessed by RAPD markers

Silva, Adailton T. da; Penna, Julio Cesar Viglioni; Goulart, Luiz Ricardo; Santos, M. Teresa; Arantes, N. E.

doi:10.1590/s1415-47572000000200014

Cited by 15 publications

(5 citation statements)

References 12 publications

(12 reference statements)

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“…All three DNA extraction methods which were used in this experiment were successful and the nematodes were tracked. Each species-specific primer was constructed to amplify DNA from the target species but to preclude the amplification of non-target Pratylenchus species As a result, in comparison with Silva et al [2] method for P. thornei and P. neglectus, modified methods of Madani et al [1] and Waeyenberg et al [3] were the best DNA extraction methods according to the band resolution which were created by gel electrophoresis and quantitative/ qualitative analysis of extracted DNA (Table 3). A common need in molecular diagnostics of lesion nematodes is to distinguish P. thornei from P. neglectus in field samples.…”

Section: Resultsmentioning

confidence: 99%

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Molecular and Morphometric Identification of P. Thornei and P. Neglectus in Southwest of Iran

Fayazi¹,

Farokhi-Nejd²,

Ahmadi³

et al. 2012

J Plant Pathol Microbiol

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Root lesion nematodes are considered as important agents of wheat yield reduction in most parts of the wheat growing areas. To elucidate disease situation in Khuzestan, a southwestern province of Iran, 40 soil & wheat root samples were collected. Morphological studies indicated that disease casual agents belong to Pratylenchus thornei and P. neglectus species. Morphometric studies showed that Differences exist in the body length compared with the studies done so far on these two species of nematode. The DNA of the two species, namely Pratylenchus thornei and P. neglectus, were extracted concidering Madani et al. [1], Silva et al. [2] and Waeyenberg et al. [3] plus some modifications. The quantity and quality of extracted DNA and its ability in DNA amplification and clearance of PCR bands were compared and the results showed that modified methods of Madani et al. [1] and Waeyenberg et al. [3] were the best methods for P. thornei and P. neglectus species. Polymerase chain reaction (PCR) and speciesspecific primers were used to identify P. thornei and P. neglectus.

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Section: Resultsmentioning

confidence: 99%

“…In the third method, modifying Silva et al [2] method, DNA was extracted from nematodes. Ten sterilized adult nematodes with 10 μl of sterilized water were put into 0.2 ml microtube with 20 μl of lyses buffer (100mM Tris-Cl, pH 8.3; 1.4mM NaCl; 20mM EDTA; and 0.1% B-Mercaptioethanol).…”

Section: Methodsmentioning

confidence: 99%

Molecular and Morphometric Identification of P. Thornei and P. Neglectus in Southwest of Iran

Fayazi¹,

Farokhi-Nejd²,

Ahmadi³

et al. 2012

J Plant Pathol Microbiol

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“…ve Meloidogyne spp. bitki paraziti nematod grupları karakterize edilmiştir (Caswell-Chen et al, 1992;Cenis, 1993;Erickson et al, 1993;Roosien et al, 1993;Chacon et al, 1994;Romero et al, 1996;Blok et al, 1997a,b;Thiery et al, 1996;Williamson et al, 1997;Yu et al, 1998;Fullaondo et al, 1999;Lecouls et al, 1999;Orui, 1999;Kaplan et al, 1999;Boiteux et al, 2000;Silva et al, 2000;Zijlstra et al, 2000;Ambrogioni & Irdani, 2001;Randig et al, 2001;Xu et al, 2001;Meher et al, 2003;Adam et al, 2007;Karajah et al, 2010). Entomopatojen nematodların tür teşhislerinde de RAPD kullanılmış ve Heterorhabditis bacteriophora, H. megidis, Steinernema glaseri, S. carpocapsae ve S. feltiae izolatları arasındaki genetik varyasyonlar belirlenmiştir (Welsh & McClelland, 1990;Williams et al, 1990).…”

Section: Rapd (Randomly Amplified Polymorphic Dna)unclassified

Nematod taksonomisinde kullanılan moleküler markörler

Gözel¹,

Yurt²,

Gözel³

2016

Türk. entomol. bült.

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Morphological characters and morphometric measurements are used for diagnosis and identification of nematodes. In some cases, reliable results can not be achieved by classic methods. Therefore, molecular markers have been applied to nematode identification. Molecular markers are methods based on DNA. In this article, information regarding to the most common molecular marker techniques including Restriction Fragment Length Polymorphism (RFLP), Amplified Fragment Length Polymorphism (AFLP), Microsatellites (SSR, STR, ISSR), Random Amplified Polymorphic DNA (RADP), Single Nucleotide Polymorphism (SNP) and DNA Sequence Analysis (mtDNA and rDNA) was given and the studies done by using these methods were reviewed.

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“…The ITS region has been widely used in the design of species-specific PCR primers and has been proven useful in the development of diagnostic tests for the detection of these nematode species [12,16,17]. RAPD fingerprints as earlier stated provide a good tool for studying genetic variation at the species level [18][19][20]. The method has been employed to distinguish between H. cruciferae and H. schachtii [9].…”

Section: Introductionmentioning

confidence: 99%

Development of a Species-Specific SCAR-PCR Assay for Direct Detection of Sugar Beet Cyst Nematode (Heterodera schachtii) from Infected Roots and Soil Samples

Jiang

Zhang

Yao

et al. 2021

Life

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Sugar beet cyst nematode (SBCN, Heterodera schachtii) is an important nematode that causes significant yield losses of 25–50% or more in most areas of sugar beet production worldwide. Rapid and accurate identification of this species is essential to support decisions on pest management. However, the difference between H. schachtii and other Heterodera spp. based on morphology is a challenging task. In the present study, a SCAR-PCR assay was developed to identify and differentiate H. schachtii in infected root and soil samples. H. schachtii-species-specific SCAR-PCR primers OPA06-HsF and OPA06-HsR were designed from the randomly amplified polymorphic DNA (RAPD) marker amplified with random primer OPA06. The developed primers specifically amplify a 922-bp fragment from the target populations but did not amplify DNA from non-target cyst nematodes including Heterodera, Globodera, Cactodera, and other related species tested in this study. The sensitivity detection indicated that 5 × 10−4 of a single cyst, 1/320 of a single second-stage juvenile (J2), or 10 pg of genomic DNA could be detected. The assay accurately identifies the different stages of H. schachtii in sugar beet and oilseed rape roots as well as a single J2 in 10 g of soil. Finally, the SCAR-PCR assay detected H. schachtii in seven samples out of the fifteen field samples. The assay will not only be useful for differentiating H. schachtii from mixed populations of Heterodera spp. but also for effective detection of the species directly from infested samples. The assay also requires no expertise in the taxonomy and morphology of the species but serves to improve the diagnosis of H. schachtii in infested fields.

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Genetic variability among and within races of Heterodera glycines Ichinohe assessed by RAPD markers

Cited by 15 publications

References 12 publications

Molecular and Morphometric Identification of P. Thornei and P. Neglectus in Southwest of Iran

Molecular and Morphometric Identification of P. Thornei and P. Neglectus in Southwest of Iran

Nematod taksonomisinde kullanılan moleküler markörler

Development of a Species-Specific SCAR-PCR Assay for Direct Detection of Sugar Beet Cyst Nematode (Heterodera schachtii) from Infected Roots and Soil Samples

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