Abstract:Root lesion nematodes are considered as important agents of wheat yield reduction in most parts of the wheat growing areas. To elucidate disease situation in Khuzestan, a southwestern province of Iran, 40 soil & wheat root samples were collected. Morphological studies indicated that disease casual agents belong to Pratylenchus thornei and P. neglectus species. Morphometric studies showed that Differences exist in the body length compared with the studies done so far on these two species of nematode. The DNA of… Show more
“…Morphometrics of females of P. crenatus is within the minimum and maximum ranges given by Castillo and Vovlas (2007) except radio 'b' which is smaller 4.2 -5.1 vs 4.9 -7.9. All morphometrics of P. neglectus are within the minimum and maximum ranges given by Castillo and Vovlas (2007) and Fayazi et al (2012) except ratio 'm' where minimum value is smaller 31 -50 vs 40.8 -48.5 (Fayazi et al, 2012). P. crenatus stylet knob width is 3±0.51 (3 -4) μm where P. neglectus stylet width is 5±0.6 (4 -6) μm; P. crenatus tail tip is crenate where as P. neglectus tail tip is without annulation.…”
SummaryThe distribution of Pratylenchus species associated with wheat crops was investigated in Bohemian region of the Czech Republic. In total twelve localities were sampled. The populations were identifi ed based on morphology and morphometrics, and further characterised based on sequences of the rDNA D2/D3 region and 18S gene. Pratylenchus crenatus was present in two localities and P. neglectus in fi ve localities. At one locality both species were detected. Sequence analysis of 18S and D2/D3 region of three populations of P. crenatus reveal no variation while fi ve populations of P. neglectus differ by 0 to 0.14 % (18S) and 0.17 to 0.50 % (D2/D3).
“…Morphometrics of females of P. crenatus is within the minimum and maximum ranges given by Castillo and Vovlas (2007) except radio 'b' which is smaller 4.2 -5.1 vs 4.9 -7.9. All morphometrics of P. neglectus are within the minimum and maximum ranges given by Castillo and Vovlas (2007) and Fayazi et al (2012) except ratio 'm' where minimum value is smaller 31 -50 vs 40.8 -48.5 (Fayazi et al, 2012). P. crenatus stylet knob width is 3±0.51 (3 -4) μm where P. neglectus stylet width is 5±0.6 (4 -6) μm; P. crenatus tail tip is crenate where as P. neglectus tail tip is without annulation.…”
SummaryThe distribution of Pratylenchus species associated with wheat crops was investigated in Bohemian region of the Czech Republic. In total twelve localities were sampled. The populations were identifi ed based on morphology and morphometrics, and further characterised based on sequences of the rDNA D2/D3 region and 18S gene. Pratylenchus crenatus was present in two localities and P. neglectus in fi ve localities. At one locality both species were detected. Sequence analysis of 18S and D2/D3 region of three populations of P. crenatus reveal no variation while fi ve populations of P. neglectus differ by 0 to 0.14 % (18S) and 0.17 to 0.50 % (D2/D3).
“…Recently, Pratylenchus haiduongensis Nguyen, Le, Nguyen, Nguyen, Liebanas & Trinh, 2017 and Pratylenchus rwandae Singh, Nyiragatare, Janssen, Couvreur, Decraemer & Bert, 2018 have been described from Vietnam and Rwanda, respectively. Morphological variation among the species of Pratylenchus has been indicated previously (Loof 1991;Ryss 2002;Fayazi et al 2012). However, accurate diagnose of Pratylenchus spp.…”
During a survey of plant-parasitic nematodes in Iran, five populations of Pratylenchus neglectus were studied by means of morphological and molecular characterization. The results showed that body length has high significant correlation with morphometric data indices such as a (r = 0.487; p ≤ 0.01), b (r = 0.355; p ≤ 0.05), c (r = 0.527; p ≤ 0.01), stylet (r = 0.625; p ≤ 0.01), excretory pore (r = 0.750; p ≤ 0.01), overlapping (r = 0.434; p ≤ 0.01) and tail length (r = 0.447; p ≤ 0.05). However, the highest correlation was detected between pharynx overlapping and postvulval uterine sac (r = −0.935; p ≤ 0.01). A molecular study of the P. neglectus populations using 28S rDNA showed close relationships of the Iranian, UK (KX683377), South Korea (KY468842) and China (JX046968). The COI of mtDNA analysis showed close relationship of the Iranian P. neglectus with the Chineese populations (KX349423; KY424105). The results indicated monophyly of P. neglectus. Illustration and measurement tables are provided for the species.
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