Chromosomal characterization of three native and one cultivated species of Lathyrus L. in Southern Brazil

Battistin, Alice; Biondo, Elaine; Coelho, Liliana Gressler May

doi:10.1590/s1415-47571999000400016

Cited by 11 publications

(10 citation statements)

References 12 publications

(19 reference statements)

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“…In South America, Lathyrus species have been found from Colombia to Tierra del Fuego, which likely migrated to the austral part of the continent through the Andean road (Burkart, 1935). All species have 2n = 2x = 14 chromosomes, with a basic number of n = x = 7 (Battistin et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Evolution of rbcL among Lathyrus and Kupicha's classification

et al. 2014

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ABSTRACT. Phylogenetic relationships in the Lathyrus genus were examined using cpDNA data, particularly data attributed to the "barcode" rbcL gene to construct a possible evolutionary scenario. Plant barcoding can be used to differentiate between species within a genus and to conserve DNA within the same species. We assessed the phylogeny of 29 species of Lathyrus using maximum parsimony, maximum likelihood and unweighted pair-group method and arithmetic mean. The classifications did not agree with current morphological and basic Lathyrus classification. Lathyrus belinensis is a new species that was not described by Kupicha; according to rbcL analysis, the species belongs in the Lathyrus genus. Additionally, the genus Lathyrus has undergone a rapid population expansion as indicated by neutral selection indices.

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Section: Introductionmentioning

confidence: 99%

Evolution of rbcL among Lathyrus and Kupicha's classification

et al. 2014

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“…Algumas das espécies produzem pouca ou nenhuma semente, as que produzem geralmente são estéreis. Desse modo, a multiplicação dessas espécies se faz via parte vegetativa: estolões, ramos e mudas (Battistin et al 2011). …”

Section: Introductionunclassified

“…Nestas regiões os genes transcricionais estão ativos, caracterizando cada uma das espécies, porém podem ocorrer sítios não ativos/silenciosos, principalmente em espécies híbridas, que não são detectados pela técnica AgNOR, necessitando aplicação da técnica de rDNA-FISH. O número e o tamanho dos nucléolos, geralmente indicam a quantidade de genes ribossomais ativos (Lovatto & Battistin, 1997;Battistin et al 1999;Sumner, 2003).…”

Section: Introductionunclassified

Caracterização citogenética para identificação dos níveis de ploidia em cinco espécies do gênero Mentha L.

Battistin

Almeida

Nogueira

et al. 2013

Rev. bras. plantas med.

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RESUMO:Neste trabalho foi feita a caracterização citogenética da: microsporogênese, tétrades, estimativa da viabilidade do pólen pelo método de coloração e contagem do número máximo de nucléolos por célula interfásica, para identificação dos níveis de ploidia, em cinco espécies do gênero Mentha L. Foram coletadas inflorescências em 30 plantas de cada espécie, em duas florações sucessivas, nos anos 2006 e 2007. As inflorescências foram tratadas em etanol-ácido acético (3:1), em temperatura ambiente durante seis horas, transferidas para álcool 70% (v/v) e conservadas em geladeira até análise. Nas análises da microsporogênese, tétrades e pólen o corante usado foi carmin propiônico 2% e na identificação dos nucléolos nitrato de prata (AgNO 3 ). Os resultados demonstraram que as cinco espécies são poliplóides. M. crispa heptaplóide (2n=7x=84) com 11 nucléolos, M. spicata tetraplóide (2n=4x=48) com 8 nucléolos, M.x gentilis pentaplóide (2n=5x=60) com 12 nucleólos, M. piperita e M.x piperita ambas hexaplóides (2n=6x=72) com 8 e 9 nucléolos respectivamente. M. spicata e M. crispa mantiveram as mais altas porcentagens de células normais na microsporogênese, na formação de tétrades e na estimativa da viabilidade do pólen por coloração, sugerindo maior estabilidade meiótica quando comparados aos demais poliplóides estudados.Palavras-chave: microsporogênese, cromossomos, poliploidia, Mentha.ABSTRACT: Citogenetic characterization to determination of ploidy level on five Mentha L. species . The cytogenetic characterization of five species of Mentha L. genus, including the data: regularity of microsporogenesis and tetrads, and polen viability, using the coloration method and the counting of the maximum number of nucleolus by interphasic cell were carried out in this study to identify the ploid levels. These analyses were performed from inflorescences collected in 30 plants of each species, during two successive florations in 2006 and 2007. Inflorescences were treated in 3:1 ethanol:acetic acid mixture at room temperature during six hours, then transferred to 70%(v/v) ethanol solution and refrigerated until the analysis. For microsporogenis, tetrad and pollen analysis, we used carmine propionic 2% (m/v) and for nucleolus identification, we used AgNO 3 solution. It was possible to observe that all five species were polyploids. M. crispa heptaploid (2n=7x=84) with 11 nucleolus, M. spicata tetraploid (2n=4x=48) with 8 nucleolus, M. x gentilis pentaploid (2n=5x=60) with 12 nucleolus, M. piperita and M. x piperita both hexaploid (2n=6x=72) with 8 and 9 nucleolus respectively. M. spicata and M. crispa kept the highest percentual values of normal cells in microsporogenesis as well as in tetrads formation and pollen viability, suggesting a higher meiotic stability when compared to the other polyploids studied.

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“…Os corantes usados foram giemsa 2% (m/v) e AgNO 3 50% (m/v), respectivamente, seguindo a metodologia proposta por BATTISTIN et al (1999) com modificações, usando para o pré-tratamento paradiclorobenzeno durante 24 horas na geladeira e o corante giemsa 2% (m/v) para os cromossomos.…”

Section: Introductionunclassified

Germinação das sementes e análise citogenética em duas espécies de palmeiras

Battistin¹,

Almeida²,

Gonçalves³

et al. 2012

RBHO

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Germination of the seeds and cytogenetic analysis in two species of palmsEuterpe edulis and Archontophoenix alexandrae are palm trees used for ornamentation purpose and palm heart extraction. Seed germination and VGI, mitotic and meiotic chromosome behavior and quantification of pollen were assessed. For each species in each location and in each substracts, 60 seeds were used. Mitotic chromosome analyses and identification of differential nucleoli were made in meristem zone of primary root. Giemsa 2% (m/v) and AgNO 3 50% (m/v) were used as dyes, respectively. Chromosomes analysis in microsporogenesis and pollen viability were made on anthers of buds and stained with carmine propionic 2% (m/v). The greatest index of germination and VGI of the seeds, in two species, were observed in samples from Maquiné in a substract containing vermiculite. A. alexandrae is a diploid with 2n=2x=32 chromosomes with karyotype m+12sm+4st+2t. E. edulis is a diploid with 2n=2x=36 chromosomes with karyotype 16m + 12sm + 8t. Both have a submetacentric pair of chromosomes with intermediate secondary constriction containing nucleoli with active ribosome genes. In these two species microsporogenesis and pollen analyses showed high percentage of normal cells at all stages of meiosis I and II resulting a high meiotic index (normal tetrads) and pollen viability, indicating that in these species the genes are expressed normally in environmental conditions in which they live and do not present problems in the formation of male reproductive cells.

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Chromosomal characterization of three native and one cultivated species of Lathyrus L. in Southern Brazil

Cited by 11 publications

References 12 publications

Evolution of rbcL among Lathyrus and Kupicha's classification

Evolution of rbcL among Lathyrus and Kupicha's classification

Caracterização citogenética para identificação dos níveis de ploidia em cinco espécies do gênero Mentha L.

Germinação das sementes e análise citogenética em duas espécies de palmeiras

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