Expression of a methionine-rich storage albumin from the Brazil nut (Bertholletia excelsa H.B.K., Lecythidaceae) in transgenic bean plants (Phaseolus vulgaris L., Fabaceae)

Abstract

Bean (Phaseolus vulgaris), an important component in the diet of people in developing countries, has low levels of the essential amino acid, methionine. We have attempted to correct this deficiency by introducing a transgene coding for a methioninerich storage albumin from the Brazil nut via biolistic methods. The transgene's coding sequence was driven by a doubled 35S CaMV promoter and AMV enhancer sequences. The transgene was stable and correctly expressed in homozygous R 2 to R 5 seeds. In two of the five transgenic lines the methionine content was significantly increased (14 and 23%) over the values found in untransformed plants.

GENETIC TRANSFORMATION AND REGENERATION OF COMMON BEAN (Phaseolus vulgaris L.) USING Agrobacterium SYSTEM

“…So far, transformation of P. vulgaris has been achieved through direct gene transfer into the apical meristem region of seedlings of one cultivar (Russell et al, 1993). Embryonic axes and the apical meristems of P. vulgaris exposed using biolistic method, however, the frequency of transgenic plants they obtained was much lower 0.03% , and Aragão et al (1999) attempted also to introduce a gene coding for methionine in bean via the same method.…”

Factors influencing electroporation-mediated gene transfer to Stylosanthes guianensis (Aubl.) Sw. protoplasts

“…Electroporation and microparticle bombardment are the principal systems for direct gene delivery to legumes (Aragão et al, 1999;Quorin et al, 1997;Potrykus, 1991).…”

Development of transgenic Sorghum bicolor (L.) Moench resistant to the Chilo partellus (Swinhoe) through Agrobacterium-mediated transformation

“…Our study demonstrated that the transfer of foreign genes to sorghum via Agrobacterium tumefaciens LBA4404 (pCAMBIA1305.1) by the shoot apex was efficient. Aragao et al, [45] transformed bean plants with a chimeric construct containing the doubled 35S CaMV promoter plus the AMV enhancer sequence, assuming that its performance would be superior to the native 35S promoter. In the present study, a large number of sorghum plants carrying Cry1C gene has been produced in APK 1 cultivar by Agrobacterium mediated transformation method using binary vector pCAMBIA1305.1/Cry1C.…”