Anticlastogenic effect of vitamin C on cisplatin in vivo

Antunes, Lusânia Maria Greggi; Darin, Joana D’Árc Castania; Bianchi, María de Lourdes Pires

doi:10.1590/s1415-47571999000300022

Cited by 20 publications

(5 citation statements)

References 22 publications

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“…In the present study, total chromosomal aberrations were decreased appreciably in AA plus cisplatin treated group at corresponding time points (Fig. 2b) showing that ascorbic acid exhibited reduction in the chromosomal aberrations induced by cisplatin which may indicate its anticlastogenic effects against cisplatin-induced mutagenic effects as has also been suggested by others (Giri et al, 1998;Antunes et al, 1999Antunes et al, , 2000aNefic, 2001). Based on these findings, it may be suggested that decrease in the frequency Res.…”

Section: Discussionsupporting

confidence: 85%

“…Cisplatin induces formation of micronuclei in bone marrow cells and chromosomal aberrations in the germinal cells of mice (Kliesch and Adler, 1987;Adler and El-Tarras, 1989) and also the micronuclei in peripheral blood lymphocytes of testicular patients with various types of cancers (Osanto et al, 1991;Elsendoorn et al, 2001). It is imperative to reduce the drug-induced genotoxicity, a goal that have been tried experimentally by administration of free radical scavengers such as antioxidants (Antunes et al, 1999(Antunes et al, , 2000aAttia, 2010;Dos Santos et al, 2012) with varying degree of success.…”

Section: Introductionmentioning

confidence: 99%

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Ascorbic Acid (Vitamin C)-Mediated Protection on Mutagenic Potentials of Cisplatin in Mice Bearing Ascites Dalton ’s Lymphoma

Amenla¹,

Prasad²

2016

Research J. of Mutagenesis

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Cisplatin is a potent anticancer drug which has been in use against a variety of cancers for a long time. The present study was carried out to assess the protective ability of ascorbic acid on cisplatin-induced mutagenic effects in tumor-bearing mice. The increase in the frequency of chromosomal aberrations, micronuclei and sperm abnormality were studied as markers of mutagenicity. Indicators of oxidative stress relatives like lipid peroxidation, reduced glutathione and the activities of enzymes such as catalase, glutathione-S-transferase and glutathione reductase were also studied to understand the possible mechanism(s) underlying this amelioration. Pre-treatment with ascorbic acid in combination group significantly reduced cisplatin-induced mutagenicity, markedly decreased lipid peroxidation along with increased level of glutathione and activities of antioxidant enzymes particularly in the liver of mice. The overall studies suggest that ascorbic acid with its antioxidant and free radical scavenging properties may play a part in its protective role in the abatement of cisplatin-induced mutagenesis and oxidative damage in the normal tissues of mice.

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Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Ascorbic Acid (Vitamin C)-Mediated Protection on Mutagenic Potentials of Cisplatin in Mice Bearing Ascites Dalton ’s Lymphoma

Amenla¹,

Prasad²

2016

Research J. of Mutagenesis

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“…Bone-marrow is the primary site in the body where hematopoietic and stromal stem cells reside and differentiate in an adult cell population. 38) Bone-marrow cells are highly sensitive to clastogenic agents and hence vulnerable to DNA damage. 39) This damage can lead to genetic rearrangements and mutations and therefore can be dangerous particularly in an undifferentiated bone-marrow cell population.…”

Section: Discussionmentioning

confidence: 99%

The Protective Effect of Naringenin on Oxaliplatin-Induced Genotoxicity in Mice

Ganaie

Jan

Khan

et al. 2019

CHEMICAL & PHARMACEUTICAL BULLETIN

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Oxaliplatin is a third generation platinum based anti-cancer drug used against various human malignancies but displays genotoxic properties against normal cells. Naringenin is a naturally occurring bioflavonoid that possesses anti-oxidant properties and has protective effects against DNA damage. The aim of this study is to examine the protective effects of naringenin on oxaliplatin-induced DNA damage in mice. A total of 50, male BALB/c mice were randomly divided equally into five groups. Oxaliplatin toxicity was induced by a single dose (7 mg/kg body weight (b.w.)) injection (intraperitoneally (i.p.)) of oxaliplatin. Naringenin was given orally for ten consecutive days at two doses, 20 mg/kg b.w. (dose I) and 40 mg/kg b.w. (dose II), to group I and group II, respectively. On the tenth day of the experiment, animals in groups III, IV, and V were given a single i.p. injection of oxaliplatin (7 mg/kg b.w.). All the animals were sacrificed 24 h after oxaliplatin treatment. The extent of genotoxicity was assessed by multiple genotoxicity assays (8-hydroxydeoxy-guanosine marker, comet, micronucleus and chromosomal aberration assays, oxidative stress-marker Glutathione evaluation) in order to determine diverse kinds of DNA damage. The results indicated that naringenin administration significantly reduced the DNA damage induced by oxaliplatin possibly due to its strong anti-oxidant properties. The results suggest that naringenin is a potential candidate for future development as a chemoprotective agent against chemotherapy associated complications.

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“…Cytotoxicity of cisplatin is predominantly attributed to its interaction with nucleophilic N7-sites of purine bases in DNA (Eastman, 1987) and evidence strongly favors intrastrand adducts to cause severe lesions (Pinto and Lippard, 1985). Cisplatin is a highly mutagenic drug, inducing chromosome aberrations in rat bone marrow cells and in peripheral blood lymphocytes of patients (Osanto, 1991;Antunes, 1999;Antunes, 2000). Prominent structural chromosome aberrations like centromeric fusion and pulverizations were noted owing to the toxic effects of cisplatin.…”

Section: Discussionmentioning

confidence: 99%

Preventive effect of Curcumin against Cisplatin-related hepatotoxicity in Swiss Albino mice (Mus musculus L.)

Saha¹,

Pal²,

Goswami³

2018

JCHPS

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Chemotherapy is a type of cancer treatment that kills rapidly proliferating cells. Being a non-specific mode of treatment, it can cause damage to healthy cells like bone marrow cells, epithelial cells, hair follicles etc., resulting in various side effects. Cisplatin, a platinum coordination complex, is a common chemotherapeutic drug that is used in the treatment of several types of cancers, including testicular, ovarian and bladder cancer. Cisplatin interferes with DNA replication by forming cross-linking of DNA in the form of 1, 2-intrastrand crosslinks with purine bases. It can also cause missense mutations and breaks in DNA. The DNA injury triggers cell death and inhibits RNA and protein synthesis, especially in rapidly dividing cells. However, it has been observed to have profound participation in neuropathy, ototoxicity and nephrotoxicity, few among many other side effects. Changes in the activity of certain serum enzymes have been implicated to cisplatin-induced hepatotoxicity. In this study, we investigated the effect of cisplatin on Swiss Albino mice (Mus musculus L.) animal model. Mice were intraperitoneally administered with cisplatin at varying sublethal doses and exposure periods. They were tested for hepatotoxicity by estimating several biochemical parameters. The mid-toxic dose was co-treated with curcumin supplementation since curcumin has prominent anti-radical, anti-inflammatory and anti-mutagenic activities. The investigation was further extended to study the effect of cisplatin on hepatic cell viability and inspect presence of chromosomal aberrations. The present study shall assist in better comprehension of cisplatin-induced hepatotoxicity and effects of curcumin to prevent related side effects.

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Anticlastogenic effect of vitamin C on cisplatin in vivo

Cited by 20 publications

References 22 publications

Ascorbic Acid (Vitamin C)-Mediated Protection on Mutagenic Potentials of Cisplatin in Mice Bearing Ascites Dalton ’s Lymphoma

Ascorbic Acid (Vitamin C)-Mediated Protection on Mutagenic Potentials of Cisplatin in Mice Bearing Ascites Dalton ’s Lymphoma

The Protective Effect of Naringenin on Oxaliplatin-Induced Genotoxicity in Mice

Preventive effect of Curcumin against Cisplatin-related hepatotoxicity in Swiss Albino mice (Mus musculus L.)

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