Genetic maps of Saccharum officinarum L. and Saccharum robustum Brandes & Jew. ex grassl

Abstract

Genetic analysis was performed in a population composed of 100 F1 individuals derived from a cross between a cultivated sugarcane (S. officinarum `LA Purple') and its proposed progenitor species (S. robustum `Mol 5829'). Various types (arbitrarily primed-PCR, RFLPs, and AFLPs) of single-dose DNA markers (SDMs) were used to construct genetic linkage maps for both species. The LA Purple map was composed of 341 SDMs, spanning 74 linkage groups and 1,881 cM, while the Mol 5829 map contained 301 SDMs, spanning 65 linkage groups and 1,189 cM. Transmission genetics in these two species showed incomplete polysomy based on the detection of 15% of SDMs linked in repulsion in LA Purple and 13% of these in Mol 5829. Because of this incomplete polysomy, multiple-dose markers could not be mapped for lack of a genetic model for their segregation. Due to inclusion of RFLP anchor probes, conserved in related species, the resulting maps will serve as useful tools for breeding, ecology, evolution, and molecular biology studies within the Andropogoneae.

Linkage mapping and genome analysis in a Saccharum interspecific cross using AFLP, SRAP and TRAP markers

“…Averaged over all markers, the expected frequency of SD markers (70%) was detected in this study using a progeny size of 100 individuals. Similar levels of SD markers have been reported in other sugarcane mapping studies using progeny sizes of 84 (Mudge et al 1996), 88 (da Silva et al 1993) and 100 (Guimarães et al 1999;Garcia et al 2006) individuals.…”

“…For the female parent, S. officinarum 'La Striped', linkage analysis failed to detect markers linked in repulsion phase but segregation analysis concluded that the genome was neither undergoing strict polysomic nor disomic segregation, thereby, concurring with previous evidence that the female parent S. officinarum 'La Striped' could be an autoalloploid. Several authors (Mudge et al 1996;Al-Janabi et al 1993;Guimarães et al 1999;Edmé et al 2006) have found evidence of repulsion phase linkages in S. officinarum, some using populations smaller than that used in this study.…”

Sugarcane Genetics and Genomics

“…The first linkage map from this population was constructed using RAPD markers, comprising 160 RAPD markers plus one morphological marker assembled into 51 linkage groups (Mudge et al 1996). A linkage map was also generated from the population with arbitrarily primed-PCR, RFLP, and AFLP markers, resulting in 74 and 65 linkage groups, respectively (Guimarães et al 1997(Guimarães et al , 1999.…”

The Sugarcane Genome Challenge: Strategies for Sequencing a Highly Complex Genome

“…Genetic maps have been produced for the ancestral species S. spontaneum (da Silva et al 1993;Dasilva et al 1995;Al-Janabi et al 1993) (Ming et al 1998b), S. officinarum (Mudge et al 1996); (Ming et al 1998b;Ming et al 2002); (Guimaraes et al 1997a) (Guimaraes et al 1999) (Aitken et al 2007) and S. robustum (Guimaraes et al 1997a;Ming et al 1998b) using different molecular marker technologies (RAPD, AFLP, RFLP and SSR). The most advanced maps for S. officinarum include 534 markers assembled in 123 LG for IJ76-545 (2n=80) (Aitken et al 2007), and 615 markers assembled into 72 linkage groups for 'Green German' (2n=97-117) (Ming et al 1998b) and for S. spontaneum IND (2n = 52-56) includes 536 markers assembled in 69 LG (Ming et al 1998b) Linkage mapping has also being conducted on modern cultivars (D'Hont et al 1994) , (Hoarau et al 2001), (Rossi et al 2003;Aitken et al 2005;Reffay et al 2005;Raboin et al 2006;Garcia et al 2006).…”