The protective effects of Baypamun®HK in mice experimentally infected with Toxoplasma gondii

Cunha, Eva Laurice Pereira; Silva, Rodrigo da; Silva, Aristeu Vieira da; Langoni, Hélio

doi:10.1590/s1413-95962004000300004

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“…All tachyzoite-inoculated mice exhibited seroconversion for IgM and IgG. However, different results have been reported in other studies (Camargo et al 1991, Camargo 1996, Cunha et al 2004, Guimarães et al 2009, Costa-Silva & Pereira-Chioccola 2010. This may have occurred because the infective dose of tachyzoites inoculated in each animal was higher in the present investigation and different strains were used in other studies.…”

Section: Discussionmentioning

confidence: 61%

Viability of Toxoplasma gondii in cattle semen cryopreserved with different concentrations of cryoprotectant

et al. 2023

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Toxoplasma gondii can be eliminated in bovine semen. Cryopreserved semen is often used due to the fact that artificial insemination in dairy and beef cattle provides benefits in terms of production. However, little is known regarding the viability and infectivity of T. gondii tachyzoites in cryopreserved bovine semen. In the present study, cattle semen negative for T. gondii were contaminated with 1 x 106 tachyzoites (RH strain) and cryopreserved with and without different cryoprotectants, such as DMSO (concentrations of 2.5%, 5.0%, 7.5%, 8.0% and 10.0%) and glycerol (2.25%, 2.5%, 3.0%, 5.0%, 7.5% and 10.0%), followed by freezing in liquid nitrogen (-196°C). After 24 hours, the samples were thawed and inoculated in 10 mice per cryoprotectant concentration. The mice were evaluated for clinical signs of toxoplasmosis (rough coat, diarrhea, hypoactivity and sudden death) as well as serum titers of IgM and IgG and the presence of tachyzoites in the peritoneal lavage. The results revealed that T. gondii remained infective in all samples. Clinical signs of toxoplasmosis were observed in the mice beginning with the 6th day post-inoculation (DPI) and 100% lethality was found between the 7th and 9th DPI. Viable tachyzoites were recovered from peritoneal exudate of dead mice (except for the control group), with higher mean of tachyzoite counts in the intraperitoneal lavage for 5% DMSO (±3.32 x 106), 8% DMSO (±3.53 x 106), 3% glycerol (±4.75 x 106), 7.5% glycerol (±6.26 x 106) and the absence of cryoprotectant (±3.11 x 106). Seroconversion occurred in the treated groups, with titers of IgG from 1:16 to 1:128 and IgM from 1:16 to 1:512. T. gondii viability and infectivity were maintained in cattle semen during 24 hours of cryopreservation at -196°C with and without cryoprotectant. However, further studies are necessary to determine whether cryopreserved semen contributes to the spread of toxoplasmosis through artificial insemination.

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Section: Discussionmentioning

confidence: 61%