Estudo morfológico e morfométrico do ovário de cutias (Dasyprocta aguti, Linnaeus, 1766)

Almeida, Mônica Marcos de; Carvalho, Maria Acelina Martins de; Filho, Miguel Ferreira Cavalcante; Miglino, María Angélica; Menezes, Danilo José Ayres de

doi:10.1590/s1413-95962003000100006

Cited by 16 publications

(19 citation statements)

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“…The agouti ovary presented yellow color, with a smooth surface containing translucent areas suggestive of developing follicles, in which corpora lutea and growing follicles did not protrude above the surface, similar to the previously descriptions in the same species (Almeida et al 2003) and in paca (Cuniculus paca, Reis et al 2011). The constituent cell types of the D. leporina ovary were similar to those described for other hystricognath rodents (Praxedes et al 2015), including the wild black agouti (Dasyprocta fuliginosa, Mayor et al 2011).…”

Section: Discussionsupporting

confidence: 88%

“…In order to improve its performance under captivity, several studies are focusing on the improvement of knowledge about the reproductive biology of the agouti, such as placentation (Rodrigues et al 2003) and morphological and morphometric studies of female reproductive organs (Almeida et al 2003, Fortes et al 2005. However, reports concerned on the development and application of biotechniques are still scarce (Mollineau et al 2008, Silva et al 2011, Castelo et al 2014, especially those focused on females (Wanderley et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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Estimation, morphometry and ultrastructure of ovarian preantral follicle population in agouti (Dasyprocta leporina)

et al. 2018

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The aim of this study was to characterize the preantral ovarian follicular population in agoutis (D. leporina) by estimating the number of follicles at each developmental category, and also describe the morphometry and the specific features of the follicle and the oocyte by using light and transmission electron microscopy. The length of each ovary was measured using a caliper rule, longitudinally sectioned into two halves and both were immediately fixed to perform the estimation of follicular population and ultrastructural analysis. The mean (±S.E.M.) population of follicular per pair of ovary was estimated at 4419.8±532.26 and 5397.52±574.91 for right and left ovaries, respectively, but no differences were observed between them. The diameters for follicles, oocyte and nuclei were: 18.62±3.40μm, 12.28±2.37μm and 6.10±0.93μm for primordial, 23.75±5.70μm, 14.22±3.00μm and 6.70±1.24μm for primary and 88.55±17.61μm, 52.85±17.56μm and 22.33±17.61μm for secondary follicles, respectively. The most of the follicles found belonged to the primordial category (86.63%), followed by primary (13.01%) and secondary (0.35%) one. Additionally, polyovular follicles were observed in all the animals and they represented 7.51% of the total follicles counted. The ultrastructural analysis showed that the oocyte presented a central and regular nuclei, displaying a homogenous mass. Among the organelles, the mitochondria were the most abundant and the oocyte Golgi apparatus was rarely observed. In conclusion, this work shows for the first time the characterization of the population of preantral follicles in the ovary of Dasyprocta leporina. Those information will be useful for further development and adaptation of biotechniques such as germplasm cryopreservation and in vitro gametes manipulation.

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Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Estimation, morphometry and ultrastructure of ovarian preantral follicle population in agouti (Dasyprocta leporina)

et al. 2018

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“…Therefore, obtaining information regarding reproduction of this species will also provide information for their rational production and consequent preservation [11]. In this regard, several studies have been performed involving the reproductive characteristics of agoutis, such as placentation [10], morphology of the ovaries [12] and oviducts [13], semen collection, and semen cryopreservation [14,15].…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural features of agouti (Dasyprocta aguti) preantral follicles cryopreserved using dimethyl sulfoxide, ethylene glycol and propanediol

Wanderley¹,

Luz²,

Faustino³

et al. 2012

Theriogenology

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].You may download, copy and otherwise use the AAM for non-commercial purposes provided that your license is limited by the following restrictions:(1) You may use this AAM for non-commercial purposes only under the terms of the CC-BY-NC-ND license.(2) The integrity of the work and identification of the author, copyright owner, and publisher must be preserved in any copy. Ultrastructural features of agouti (Dasyprocta aguti) preantral follicles cryopreserved using dimethyl sulfoxide, ethylene glycol and propanediol AbstractThe objective was to develop an efficient protocol for cryopreservation of agouti (Dasyprocta aguti) ovarian tissue. Agouti ovarian fragments were placed, for 10 min, in a solution containing MEM and fetal bovine serum plus 1.5 M dimethyl sulfoxide (DMSO), ethylene glycol (EG) or propanediol (PROH); some of those fragments were subsequently cryopreserved in a programmable freezer. After exposure and/or thawing, all samples were fixed in Carnoy prior to histological analysis. To evaluate ultrastructure, follicles from the control and all cryopreserved treatments were fixed in Karnovsky and processed for transmission electron microscopy. After exposure and freezing, there was a significant decrease in the percentage of morphologically normal preantral follicles in all treatments when compared to the control (92.67 ± 2.79, mean ± SD). However, there were no significant difference when the exposure and freezing procedures were compared using the same cryoprotectant. Moreover, there was no significant difference among cryoprotectants at the time of exposure (DMSO: 64.7 ± 3.8; EG: 70.7 ± 11.2, PROH: 63.3 ± 8.5) or after freezing (DMSO: 60.6 ± 3.6, EG: 64.0 ± 11.9; PROH: 62.0 ± 6.9). However, only follicles frozen with PROH had normal ultrastructure. In conclusion, preantral follicles enclosed in agouti ovarian tissue were successfully cryopreserved using 1.5 M PROH, with satisfactory maintenance of follicle morphology and ultrastructure.

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“…Regarding sperm morphometry, the G. spixii sperm presents a head that occupies 9.4% on average of the total sperm length, 48, 87 ± 0.1 μm (Silva et al, 2017a). Regarding female, ovaries are dorso-ventrally flattened ovoid structures, with length of 2.7 cm on average (Praxedes et al, 2017), located in the abdominal cavity, in the sublumbar region, surrounded by adipose tissue (Almeida et al, 2003). The ovarian follicles in several stages of development are located in the cortical region, while medullary region is comprised of vessels and fine tissues (Santos et al, 2014b).…”

Section: Reproductive Aspectsmentioning

confidence: 99%

Conservation of germplasm derived from spix’s yellow-toothed cavies (Galea spixii WAGLER, 1831)

Silva¹,

Silva²,

Paxedes³

2017

SPERMOVA

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RESUMENLa importancia ecológica y económica de la Galea spixii, un roedor histricomorfos, ha despertado el interés de los investigadores en el desarrollo de estudios centrados en la conservación de su germoplasma. En este sentido, esta revisión presenta información publicada sobre métodos para obtener y caracterizar gametos masculinos y femeninos. Además, se muestran datos interesantes sobre la congelación de los espermatozoides y la vitrificación del tejido testicular y ovárico. Estos estudios prometedores evidencian las posibilidades de mejorar otras técnicas de reproducción asistida centradas en la multiplicación y conservación de G. spixii.Palabras clave: Rodentia, cavia, vida Silvestre, espermatozoide, ovocito. ABSTRACTThe ecological and economical importance of the Galea spixii, a hystricognath cavid rodent, has aroused the interest of the researchers at developing studies focused on the conservation of its germplasm. In this sense, this review presents published information regarding methods for obtaining and characterizing both male and female gametes. Moreover, interesting data regarding sperm freezing and vitrification of testicular and ovarian tissue are shown. Such promising studies evidence the possibilities for improving other assisted reproductive techniques focused on G. spixii multiplication and conservation.

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Estudo morfológico e morfométrico do ovário de cutias (Dasyprocta aguti, Linnaeus, 1766)

Cited by 16 publications

References 0 publications

Estimation, morphometry and ultrastructure of ovarian preantral follicle population in agouti (Dasyprocta leporina)

Estimation, morphometry and ultrastructure of ovarian preantral follicle population in agouti (Dasyprocta leporina)

Ultrastructural features of agouti (Dasyprocta aguti) preantral follicles cryopreserved using dimethyl sulfoxide, ethylene glycol and propanediol

Conservation of germplasm derived from spix’s yellow-toothed cavies (Galea spixii WAGLER, 1831)

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