Cinética da maturação nuclear in vitro de oócitos bubalinos

Santos, Simone do Socorro Damasceno; Dantas, J. K.; Miranda, M. S.; Biondi, Flávia; Ohashi, Otávio Mitio

doi:10.1590/s1413-95962002000500009

Cited by 5 publications

(6 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Ultrastructure study of Egyptian buffalo oocytes revealed that the polar body was extruded after 24 h of culture as reported by earlier studies (Yadav et al, 1997;Santos et al, 2002;Nandi et al, 2002;Gasparrini et al, 2008). On the contrary, Mondadori et al (2010) found that the first polar body was observed after 12 h of culture.…”

Section: Discussionsupporting

confidence: 64%

Fine structure of Egyptian buffalo oocytes (Bubalus bubalis) during different in vitro maturation periods using transmission electron microscopy (TEM)

Barakat¹

2012

Afr. J. Biotechnol.

View full text Add to dashboard Cite

This study was carried out to illustrate the ultrastructure of Egyptian buffalo oocytes through in vitro maturation (IVM) period (24 h). Selected cumulus oocytes complexes (COCs) were divided into three groups in relation to the time of maturation (8, 16 and 24 h >). After different maturation intervals, the matured oocytes were examined by light and transmission electron microscope (TEM). Results showed that the cumulus cells were close to each other and zona plucida (ZP) in the first group than the second and third group, lipid droplets (LD) appeared normal and nearly from plasma membrane in the group of oocytes matured in vitro for 8 h than oocytes matured in vitro for 24 h, microvilli (Mv) appeared with more number, higher thickness and penetrating the ZP and cytoplasm in the first group than other two groups, and mitochondria (M) in the first group were located near the plasma membrane, while in the third group it appeared as individual and scattered through the oocyte cell. Finally, the first polar body was observed in the third group only. This study showed the ultra-structural concepts of Egyptian buffalo oocytes during different IVM periods, and provided insights about the changes that occurred in oocytes during maturation process in the bovine species.

show abstract

Section: Discussionsupporting

confidence: 64%

Fine structure of Egyptian buffalo oocytes (Bubalus bubalis) during different in vitro maturation periods using transmission electron microscopy (TEM)

Barakat¹

2012

Afr. J. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…In most oocytes (three of five) studied, metaphase II stage was achieved much earlier (12 h of IVM) in contrast to earlier reports (Yadav et al . 1997; Santos et al ., 2002; Nandi et al ., 2002b; Gasparrini et al ., 2008). From our point of view, as this experiment was not designed to determine oocyte maturation time point, this finding does not have a high biological value, because the number of observed oocytes (five structures) was low and the literature describes great biological variability in female buffalo reproductive patterns.…”

Section: Discussionmentioning

confidence: 99%

Ultrastructure of in vitro oocyte maturation in buffalo (Bubalus bubalis)

Mondadori

Santin²,

Fidelis³

et al. 2010

Zygote

View full text Add to dashboard Cite

The objective of the present study was to describe ultrastructural changes in the nucleus and cytoplasmic organelles during in vitro maturation (IVM) of buffalo cumulus-oocyte complexes (COCs). The structures were collected by ovum pick-up (OPU). Some COCs, removed from maturation medium at 0, 6, 12, 18 and 24 h, were processed for transmission electron microscopy. The average number of COCs collected by OPU/animal/session was 6.4, and 44% of them were viable. Immature oocytes had a peripherally located nucleus, Golgi complex and mitochondrial clusters, as well as a large number of coalescent lipid vacuoles. After 6 h of IVM, the oocyte nucleus morphology changed from round to a flatter shape, and the granulosa cells (GC) lost most of their contact with zona pellucida (ZP). At 12 h the first polar body was extruded and the aspect of lipid droplet changed to dark, probably denoting lipid oxidation. Cortical granules were clearly visible at 18 h of maturation, always located along the oocyte periphery. At 24 h of IVM the number of cortical granules increased. Ultrastructure studies revealed that: (1) immature oocytes have a high lipid content; (2) the perivitelline space (PS) increases during IVM; (3) Golgi complexes and mitochondrial clusters migrate to oocyte periphery during IVM; (4) 6 h of IVM are enough to lose contact between GC and ZP; (5) the oocyte lipid droplets' appearance changes between 6 and 12 h of IVM.

show abstract

“…Buffalo oocytes reach high maturation rates (~ 80%) when taken to culture in laboratory conditions, similar to those obtained in bovine species (Santos et al, 2002b;Suresh et al, 2009;Gimenes, 2010). Traditionally, the medium and culture conditions are the same as those used for bovine oocytes (Galli et al, 2003).…”

Section: In Vitro Maturation (Ivm)mentioning

confidence: 66%

“…According to Surresh et al (2009), the best maturation, cleavage and embryo production rates in buffalo species are achieved when oocytes with a proper cumulus cell layer from large follicles and in the cold seasons of the year are cultured in TCM-199 media supplemented with fetal bovine serum (FBS), FSH and cysteamine (Suresh et al, 2009). In turn, the time required for complete nuclear maturation of oocytes in vitro , which means the arrival of the chromosomes to Metaphase II stage, could be from 18 to 24 h (Santos et al, 2002a;Gasparrini et al, 2008). Particularly, the increase in the maturation period of buffalo oocytes in the laboratory is related to a decrease in the blastocyst production rates (Oba and Camargos, 2011).…”

Section: In Vitro Maturation (Ivm)mentioning

confidence: 99%

In vitro embryo production in buffaloes: from the laboratory to the farm

et al. 2019

Self Cite

View full text Add to dashboard Cite

Transvaginal follicular aspiration technique together with in vitro embryo production are the biotechnological alternatives currently available to support genetic improvement breeding programs in buffalo species. However, aspects related to animal management, lack of knowledge of the metabolic needs and biochemical peculiarities of gametes and embryos, as well as the reproductive physiology characteristics have hampered progress in the results. Despite the low availability of good quality oocytes collected after OPU in donors as a physiological characteristic of buffalo species, high rates of oocyte maturation, modest embryo cleavage, blastocyst production and pregnancy rates after transvaginal embryo transfer in recipients could be obtained in buffalo in vitro embryo production programs. The results of implementing an in vitro embryo production program in buffaloes in the northern region of Pará state, Brazil, and results published by other groups demonstrate the feasibility of implementing this biotechnology in the routine of breeding programs. Nevertheless, in order to achieve better and consistent results, it is necessary to deepen the knowledge on the peculiarities of reproductive biology in this specie. Selection of donor animals based on ovarian size and ovarian follicular reserve and on the rate of blastocyst production is presented as an effective alternative to increase the efficiency of the in vitro embryo production technique applied to the buffalo species.

show abstract

Cinética da maturação nuclear in vitro de oócitos bubalinos

Cited by 5 publications

References 13 publications

Fine structure of Egyptian buffalo oocytes (Bubalus bubalis) during different in vitro maturation periods using transmission electron microscopy (TEM)

Fine structure of Egyptian buffalo oocytes (Bubalus bubalis) during different in vitro maturation periods using transmission electron microscopy (TEM)

Ultrastructure of in vitro oocyte maturation in buffalo (Bubalus bubalis)

In vitro embryo production in buffaloes: from the laboratory to the farm

Contact Info

Product

Resources

About