Vitrificação de oócitos e embriões bovinos produzidos in vitro e expostos à citocalasina B

Mezzalira, Alceu; Viera, Arnaldo Diniz; Barbieri, D. P.; Machado, Marcela Aparecida Guerreiro; Neto, André Thaler; Bernadi, Mari Lourdes; Silva, Carlos Antônio Mondino; Rubin, M. I. B.

doi:10.1590/s1413-95962002000500008

Cited by 4 publications

(5 citation statements)

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“…With GV vitrified oocytes in cooled nitrogen, the cleavage rate obtained (41.2%) is very close to the 50% observed by VAJTA et al (1998) and 49.1% by MEZZALIRA et al (2002) with vitrified MII oocytes, demonstrating a progress in the GV bovine oocytes cryopreservation. Our cleavage rates (33.9 to 53.6%) are satisfactory and indicate high survival of bovine oocytes following vitrification.…”

Section: Discussionsupporting

confidence: 86%

“…Unfortunately blastocyst rates were very low (4.2 to 10.6%), suggesting that cleavage is not an accurate parameter to evaluate the viability of vitrified oocytes. Developmental capacity of cleaved oocytes following vitrification is lower than that of unvitrified control oocytes (FUKU et al, 1995;MEZZALIRA et al, 2002). It suggests the occurrence of injuries that may not avoid the cleavage, but reduces further embryo development (FUKU et al, 1995;HOCHI et al, 1998;MEZZALIRA et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

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Vacuum-cooled liquid nitrogen increases the developmental ability of vitrified-warmed bovine oocytes

et al. 2006

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Vacuum-cooled liquid nitrogen increases the developmental ability of vitrified-warmed bovine oocytes

et al. 2006

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“…As taxas de clivagem observadas entre os grupos vitrificados foram semelhantes, sendo MV (31,8%), PB (32,64%), OPS (31,43%) e PM (43%). Estas taxas se assemelham às obtidas em trabalhos previamente desenvolvidos no laboratório, ou seja, 46 a 49% [24] e 33,9 a 41,2% [20], sendo ainda comparáveis às obtidas por outros autores após a vitrificação de oócitos bovinos imaturos (55,7%) [6] ou maturados (49,1% [17] e 50% [22]). A clivagem é um bom indicativo de sobrevivência após a criopreservação, e as taxas obtidas neste estudo e nos demais trabalhos atestam a viabilidade do processo.…”

Section: Discussionunclassified

Aumento na sobrevivência após vitrificação de oócitos bovinos imaturos em recipientes com maior condutividade e nitrogênio super-resfriado

Bunn

Cruz

Pedrazzi³

et al. 2018

Acta Scientiae. Vet.

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2008. Aumento na sobrevivência após vitrificação de oócitos bovinos imaturos em recipientes com maior condutividade... Acta Scientiae Veterinariae. 36(3): 255-261. RESUMOA condutividade térmica do recipiente pode alterar a velocidade de resfriamento e reaquecimento, durante a criopreservação de oócitos, influenciando sua viabilidade. Recipientes de diferentes condutividades térmicas foram comparados na vitrificação de oócitos bovinos. Oócitos imaturos (n=1454) foram expostos a uma solução de 20% EG + 20% DMSO e 0,5 M Sacarose, envasados em PM (palheta metálica inoxidável, n=265), MV (micropipeta de vidro, n=279), PB (palheta cortada em bisel, n=280), OPS (open pulled straw, n=272) e vitrificados em nitrogênio submetido ao vácuo. O reaquecimento foi realizado por 5 minutos de exposição a cada solução de sacarose (0,30 e 0,15 M), aquecidas a 35ºC. Como controle, 358 oócitos foram mantidos frescos, sem vitrificar. Os oócitos foram maturados e fecundados, sendo os prováveis zigotos cultivados em meio SOFaaci, a 39ºC, com 5% CO 2 , 5% O 2 e 90% N 2 , em umidade saturada. Não foram verificadas diferenças nas taxas de clivagem entre os tratamentos, que foram inferiores (p < 0,05) ao grupo controle. Nos grupos vitrificados, maior taxa de blastocisto foi obtida com o tratamento PM (10,2%), que foi superior (p < 0,05) aos tratamentos OPS (6,1%) e PB (6,1%), não diferindo do grupo MV (8%), embora com tendência de ser superior (p < 0,1). Os grupos tratados tiveram semelhantes taxas de eclosão que, todavia, foram inferiores ao controle. Conclui-se que o aumento da velocidade de resfriamento melhora a viabilidade após a vitrificação de oócitos bovinos imaturos, sendo que as palhetas metálicas, de maior condutividade, são mais efetivas do que as palhetas plásticas (OPS e PB), embora sem diferir da micropipeta de vidro (MV).Descritores: vácuo, micropipeta de vidro, palheta de metal, OPS, oócitos, bovinos. ABSTRACTThe thermal conductivity of the container can affect the cooling and warming rate during the oocyte cryopreservation, influencing its viability. Containers of different thermal conductivities were compared for the vitrification of bovine oocytes. Immature oocytes (n=1454) were exposed to a 20% EG + 20% DMSO and 0.5 M Sucrose solution, loaded into PM (stainless straws, n=265), MV (glass micropipettes, n=279), PB (beveled-cut straws, n=280), OPS (open pulled straws, n=272) and vitrified in nitrogen submitted to vacuum. Rewarming was performed by 5 min exposure to each sucrose solution (0.30 and 0.15 M), at 35ºC. As control, 358 oocytes were used fresh (non-vitrification). Oocytes were in vitro-matured and fertilized, with the presumptive zygotes being cultured in SOFaaci medium at 39ºC, with 5% CO 2 , 5% O 2 and 90% N 2 , in saturated humidity. No differences in cleavage rates were detected between treatments, being all lower than the control group (p < 0.05). In the vitrified groups, the highest blastocyst rate was observed in the PM treatment (10.2%), which in turn was higher (p < 0.05) than both the OPS (6.1%) and PB t...

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“…Initially, they were used for cattle and pig embryo vitrification to prevent cellular disruption, specifically to the embryonic cytoskeleton during and after cryopreservation [218][219][220] with either no benefits [221] or with positive results [220,222]. These molecules induce microfilament depolymerization before and during vitrification [219], acting as microfilaments inhibitors, thus preventing actin polymerization and making the plasmatic membrane and cytoskeleton more elastic.…”

Section: Methods Of Improving the Cryopreservation Of Sheep Embryosmentioning

confidence: 99%

Cryopreservation of Sheep Produced Embryos – Current and Future Perspectives

Romão¹,

Marques²,

Bettencourt³

et al. 2016

Insights From Animal Reproduction

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Due to economical and scientific limitations, sheep embryo reproductive technologies are less commercially applied than in other animal species. However, it is very clear that, in the near future, those techniques are expected to have a central role in animal production as a consequence of genetic and reproductive demands. One drawback is that results obtained after sheep embryo cryopreservation are unattractive for commercial purposes. It is expected that a successful cryopreservation of sheep embryos can push forward all other reproductive biotechnologies in this species, such as multiple ovulation and embryo transfer (MOET), artificial insemination, or in vitro production of embryos. This paper tries to discuss the current and future perspectives of cryopreservation of in vivo-and in vitro-produced sheep embryos concerning advantages and limitations for its practical use and possible solutions for improving methods to allow a higher survival rate of cryopreserved embryos.

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Vitrificação de oócitos e embriões bovinos produzidos in vitro e expostos à citocalasina B

Cited by 4 publications

References 24 publications

Vacuum-cooled liquid nitrogen increases the developmental ability of vitrified-warmed bovine oocytes

Vacuum-cooled liquid nitrogen increases the developmental ability of vitrified-warmed bovine oocytes

Aumento na sobrevivência após vitrificação de oócitos bovinos imaturos em recipientes com maior condutividade e nitrogênio super-resfriado

Cryopreservation of Sheep Produced Embryos – Current and Future Perspectives

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