Histopathologic and mycologic aspects of experimental infection of guinea pigs with Microsporum canis

Cavalcanti, Josemara Neves; Guerra, José Luiz; Gambale, Walderez; Corrêa, Benedito; Paula, Claudete Rodrigues

doi:10.1590/s1413-95962002000500004

Cited by 11 publications

(7 citation statements)

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“…Various methods have previously been used to render the skin more susceptible to infection, such as scarification, abrasion with a scalpel and inoculate fixed to the flank by bandaging for approximately 24hs [10]. In these studies, the experimental infections are commonly induced in the posterior dorsal region, cheek pouch, hind thigh and other skin areas [1,[7][8][9], causing suffering and stress to the animals.…”

Section: Canis Trichopyton Mentagrophytes Andmentioning

confidence: 99%

“…Experimental infection is a valuable method of studying the pathogenesis of fungal infections, to evaluate the prophylactic efficacy of antifungal therapeutic drugs and to study the immunology of dermatophytosis [1][2][3]. Dermatophytes are a group of highly specialized fungi that infect keratinized tissues, such as the hair, nail and stratum corneum of humans and other animals.…”

Section: Introductionmentioning

confidence: 99%

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Experimental Dermatophytosis on the Outer Ear of Guinea Pigs: A Model That Mimics Natural Infection

Fontenelle¹,

Morais²,

Brito³

et al. 2014

RUVRV

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In the present study a new model of experimental dermatophytosis was tested. The aim was to achieve maximum similarity to a natural infection through a procedure that is easy to carry out. The experimental infection was induced by a single inoculation of 0.5 mL of a suspension with hyphal fragments of T. mentagrophytes var. mentagrophytes on the outer ear of guinea pigs. The first signs of infection were observe on the seventh day after inoculation. The experiment demonstrated that this method, besides being effective, causes less stress and the clinical aspects the lesions here were similar to natural lesions.

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Section: Canis Trichopyton Mentagrophytes Andmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Experimental Dermatophytosis on the Outer Ear of Guinea Pigs: A Model That Mimics Natural Infection

Fontenelle¹,

Morais²,

Brito³

et al. 2014

RUVRV

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show abstract

“…They are prone to many bacterial, fungal or parasitic skin diseases if proper care is not taken. Among them dermatophytosis is one of the most common diseases [1]. Dermatophytosis is a superficial cutaneous infection with one or more of the fungal species in the keratinophilic genera Microsporum, Trichophyton, or Epidermophyton [2,3].…”

Section: Introductionmentioning

confidence: 99%

An Over View of Dermatophytosis in Rabbits

Wisal¹

2019

SAJRM

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Dermatophytosis is a fungal infection of the skin caused by dermatophytes-filamentous fungi which have ability to invade the epidermis and keratinized structure derived from it such as hair or nails. Rabbits are one of dermatophytes host; young rabbit below 12 months of age were more frequently affected with the disease. T. mentagrophytes is the most common dermatophytes isolated species. The disease can be diagnosed by direct examination, fungal culture, skin biopsy sero and molecular diagnosis methods. This overview forecast more light of the different aspects of this disease.

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“…A dermatofitose é uma das dermatopatias mais comuns na clínica veterinária e humana, causada por um grupo de fungos que possuem a capacidade de invadir e se desenvolver em estruturas queratinizadas e semiqueratinizadas, os dermatófitos (Liu et al 2000, Cavalcanti et al 2002, Liu et al 2002.…”

Section: Introductionunclassified

Padronização de multiplex PCR para detecção de dermatófitos em pelos e crostas de cães e gatos

et al. 2018

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RESUMO: Objetivou-se padronizar uma reação do tipo multiplex PCR (mPCR) para detectar Microsporum canis, Microsporum gypseum e o complexo Trichophyton mentagrophytes em amostras de pelos e/ou crostas de cães e gatos. 250 amostras de pelos e/ou crostas de cães e gatos foram analisadas por meio de exame direto e cultura, o DNA das mesmas foi extraído para mPCR. Primers foram desenhados e como controle positivo da reação utilizou-se o DNA extraído de colônias de M. canis (URM 6273), M. gypseum (URM 6921) e T. mentagrophytes (URM 6211), provenientes da Coleção de Culturas (Micoteca URM), Departamento de Micologia, Centro de Ciências Biológicas da Universidade Federal de Pernambuco (CCB/UFPE). Como controles negativos de reação, utilizou-se água destilada esterilizada e DNA extraído de Alternaria sp. para verificar a especificidade dos primers. Do total de amostras analisadas, 15 (6%) foram identificadas, em cultura, como dermatófitos, e destas, 10 foram M. canis, três M. gypseum e dois T. mentagrophytes (complexo). Destas 15 amostras positivas, 11 (73,3%) foram detectadas por meio da mPCR. Além destas, seis outras, negativas em cultura, foram identificadas como M. gypseum. Verificou-se uma boa concordância entre os resultados da cultura e mPCR (Kappa: 0,66). O protocolo padronizado neste estudo pode ser utilizado como um método de triagem, por apresentar uma sensibilidade maior que a da cultura, usado paralelamente aos exames de rotina, permitindo um diagnóstico em menor tempo.

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Histopathologic and mycologic aspects of experimental infection of guinea pigs with Microsporum canis

Cited by 11 publications

References 0 publications

Experimental Dermatophytosis on the Outer Ear of Guinea Pigs: A Model That Mimics Natural Infection

Experimental Dermatophytosis on the Outer Ear of Guinea Pigs: A Model That Mimics Natural Infection

An Over View of Dermatophytosis in Rabbits

Padronização de multiplex PCR para detecção de dermatófitos em pelos e crostas de cães e gatos

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