Experimental Dermatophytosis on the Outer Ear of Guinea Pigs: A Model That Mimics Natural Infection

Abstract

In the present study a new model of experimental dermatophytosis was tested. The aim was to achieve maximum similarity to a natural infection through a procedure that is easy to carry out. The experimental infection was induced by a single inoculation of 0.5 mL of a suspension with hyphal fragments of T. mentagrophytes var. mentagrophytes on the outer ear of guinea pigs. The first signs of infection were observe on the seventh day after inoculation. The experiment demonstrated that this method, besides being effective, causes less stress and the clinical aspects the lesions here were similar to natural lesions.

Antifungal activity of alexidine dihydrochloride in a novel diabetic mouse model of dermatophytosis

“…Disease progression in the diabetic mouse model paralleled that of previous studies established in the immunocompetent guinea pig model. However, the presence of diabetes as a physiologically relevant-predisposing factor induced far more severe lesions, peaking at days 7-10 versus days 10-15 in other published models (Saunte et al, 2008;Fontenelle et al, 2014). In fact, such erythematous and hyperkeratotic manifestations are frequently witnessed in diabetic patients or those receiving immunosuppressive corticosteroid treatment (Fraga-Silva et al, 2015;Poojary et al, 2019).…”