Aspectos histopatológicos de Biomphalaria glabrata Say, 1817, hospedeiro intermediário de Schistosoma mansoni, submetida a Niclosamida

Pilé, Edwin; Pastor, Nanci; Santos, Jose Augusto dos; Barros, Juliana Sao Luiz de

doi:10.1590/s1413-95962002000400010

Cited by 4 publications

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“…In addition, we also observed dark substances without structures in the digestive gland epithelium, similar to the substances described by Adewunmi and Ogbe [34]. We observed in uninfected and infected B. glabrata the same toxic effect in the tissue described by Pile et al [37,38] in Lymnaea columella. In this study, using sublethal concentrations of E. milii latex, the intermediate host did not die, but the products altered the tissue structure, probably related to toxic effect of this product, which directly interfered in hemocyte profile of the tissues.…”

Section: Discussionsupporting

confidence: 90%

“…In the relation the toxic effect of phytochemicals, several works have reported snail tissue damages caused by aqueous plant extracts [34][35][36][37][38]. Using aqueous extract of E. milii in Lymnaea columella, Pile et al [37,38] observed lesions characterized by degeneration, necrosis and accumulation of liquid in the digestive gland and kidney in specimens submitted to 0.47 ml/l of latex, similar to our findings. We also observed lesions in the digestive gland and mantle, close to the kidney, observed by different colorations in the digestive gland and by edema in the epithelium of the kidney, suggesting liquid accumulation.…”

Section: Discussionsupporting

confidence: 88%

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Destruction of secondary Schistosoma mansoni sporocysts in Biomphalaria glabrata after phytochemical exposure

Friani

Costa

Mota

et al. 2020

Preprint

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Background: Schistosomiasis is a neglected tropical disease and affects over 200 million people worldwide. The snail Biomphalaria glabrata is one of the intermediate hosts of S. mansoni . The aim of this work was to verify the action of Euphorbia milii var. hislopii latex in the hemocytes profile and histopathology of B. glabrata infected by S. mansoni .Methods: Uninfected and infected snails were exposed to sublethal concentrations of E. milii latex for 24 hours (1.0 mg/l less than the LC 50 ) and after this time were analysed.Results: The survival rate was 88.5% for the uninfected snails and 66.6% for the infected and exposed snails. In the snails infected by S. mansoni , the exposure to E. milii latex promoted proliferation of hemocytes in the tentacles, mantle, digestive gland, kidney and ovotestis. In the digestive gland and the kidney, granulomatous reactions occurred around the sporocysts and caused their destruction. Proliferation of hemocytes in the kidney and digestive gland and edema in the mantle area were also observed in the uninfected and exposed snails. The number of circulating hemocytes from the group infected and exposed to E. milii latex was significantly higher than in the other groups. Three types of hemocytes were found: hyalinocytes, granulocytes and blast-like cells, and in all the groups the proportion of hyalinocytes was higher than the other types. There was no significant difference among the cell types and the different groups analyzed. Conclusions: We conclude that the sublethal concentration of E. milii latex influenced the cellular immune response of the susceptible B. glabrata strain to infection by S. mansoni , promoting the destruction of parasites.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 88%

Destruction of secondary Schistosoma mansoni sporocysts in Biomphalaria glabrata after phytochemical exposure

Friani

Costa

Mota

et al. 2020

Preprint

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“…The use of latex at a sub-lethal concentration (1 mg/ l = LD 50 ) suggests the occurrence of injury to the organs of B. glabrata. Araújo et al (2002) and Pile et al (2002) demonstrated histological changes, describing degeneration and tissue death in snails submitted to intoxication from products with molluscidal activity. Bode et al (1996) observed that in B. glabrata exposed to an extract of Tetrapleura tetraptera, there was an increase in the number of secretory cells and reduction in the number of digestive cells in the digestive gland, and also an intense autolysis of membranous structures, such as those of the Golgi complex, mitochondria and endoplasmic reticulum.…”

Section: Discussionmentioning

confidence: 99%

Physiological changes in Biomphalaria glabrata say, 1818 (Pulmonata: Planorbidae) caused by sub-lethal concentrations of the latex of Euphorbia splendens var. hislopii N.E.B (Euphorbiaceae)

Mello-Silva

Vasconcellos

Pinheiro

et al. 2006

Mem. Inst. Oswaldo Cruz

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Molluscides have been used as one of the strategies to control schistosomiasis. Many plant extracts with molluscidal effects have been tested, but the action of the latex of Euphorbia splendens var. hislopii is considered the most promising because it meets the recommendations of the World Health Organization (WHO). The objective of this study was to determine the lethal dose and identify the effects of the different doses of latex of E. splendens var.hislopii on the physiology of Biomphalaria glabrata submitted to treatment for 24 h. The concentrations of glucose, uric acid and total proteins in the hemolymph and of glycogen in the digestive gland and cephalopodal mass were determined. The LD 50 value was 1 mg/l. The highest escape index was found to be at a concentration of 0.6 mg/l. The results showed that the latex of E. splendens var. hislopii caused a sharp reduction in the reserves of glycogen in the digestive gland and elevation of the protein content in the hemolymph of B. glabrata. Key words: plant molluscide -Euphorbia splendens var. hislopii -Biomphalaria glabrataThe use of molluscides as one of the strategies to control schistosomiasis began in Brazil in 1976, with the creation of the Special Schistosomiasis Control Program by the National Health Foundation (Machado 1982). The product used in the program was niclosamide, an ethanolamine salt of 2',5-dichloro-4'-nitrosalicylanilide, manufactured under the trade name Bayluscide  , whose efficacy had previously been established (Gonnert 1961). Application of this product caused biocidal action on non-target plants and animals (Andrews et al. 1983), besides causing genotoxicity and carcinogenic effects (Vega et al. 1988). The high cost (Pieri 1995), the possibility of recolonization of breeding grounds (Sarquis et al. 1997(Sarquis et al. , 1998, and the ecological toxicity of this product were limitations on its use as an official molluscide in government programs to control schistosomiasis.At the same time, various plants were tested as natural molluscides. Jurberg et al. (1989) (Mendes et al. 1984). Of these, E. splendens stands out for its molluscidal activity in doses under 0.5 mg/l, eight times smaller than the lethal dose for fish (Vasconcellos & Schall 1986).Some questions have arisen in the selection of plant molluscides, such as: toxicity, availability, annual growth, adaptability to different local conditions, and location of molluscidal activity in parts of the plant that easily regenerate, such as the leaves. To be eligible for use, a product must be storable and remain viable for at least one year; be physically and chemically stable; have ethnobotanical value; and be easy to extract and apply, preferably in aqueous extracts (Kloos & McCullough 1982). Considering all these criteria, in 1998 the Oswaldo Cruz Institute (IOC, Fiocruz, RJ) obtained a biotechnology patent on a method to collect, extract and apply E. splendens var. hislopii latex as a molluscide (Vasconcellos 2000).In the published works on plants with molluscidal action and ...

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“…desenvolvimento do molusco L. columella durante a maioria dos meses do ano, e o número de moluscos foi constante durante os meses de colheita, na pastagem onde se haviam fontes permanentes de água.Sendo, estes moluscos, vetores para a transmissão da fasciolose hepática, o controle da transmissão desta doença pode ser realizado com a redução de sua população, com o auxílio do uso de agentes moluscicidas(PILE et al, 2002). Essas substâncias são uma estratégia promissora, uma vez que o foco para o combate da fasciolose não está apenas na eliminação do parasito, bem como no controle do vetor, interrompendo-se o ciclo evolutivo do parasito e consequentemente, o aparecimento de novos casos(CANTANHEDE et al, 2010) PINHEIRO et al, 2003)…”

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Plantas Do Cerrado Brasileiro Como Possíveis Agentes Moluscicidas

Gardioli¹,

Américo²,

Ignacchiti³

et al. 2017

RGEO-UFJF

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The biome Cerrado is characterized as the most diversified savannah in the world, containing about one third of Brazilian biodiversity. In this study we used the typical vegetal species of this biome, Davilla elliptica and Davilla nitida with the aim of obtaining hydroalcoholic extracts for tests against Lymnaea columella snails intermediate hosts of the parasite Fasciola hepatica, which causes fasciolosis. This parasitosis is a high importance disease in the veterinary medicine due to the great economic losses that it causes. Among the several methods used to reduce the number of cases of the disease, the control of snail"s populations may be a promising measure. In addition, the search to discovery new molluscicides from vegetal species has been increasing, since the use of niclosamide (N-(2'-chloro-4'nitrophenyl) -5-chlorosalicylanilide), only substance to help control the proliferation of snails can generate serious environmental problems such as toxicity to other species. Thus the objective of this study was to analyze the efficiency of hydroalcoholic extracts of D. elliptica, D. nitida, as possible molluscicidal agents for the L. columella species. The hydroalcoholic extracts of D elliptica and D. nitida presented molluscicidal activity against L. columella species, being that D. elliptica presented more promising results, triggering both molluscicidal and ovicidal effects, which suggests that this vegetal extract can be used in tests as an alternative control of this parasitosis. TABELA 2Teste de inibição de ovoposição ConclusõesAssim, pode-se concluir que os extratos hidroalcoólicos de D. nitida e D elliptica apresentaram atividade moluscicida contra a espécie L. columella, sendo que D. elliptica apresentou resultados mais promissores, desencadeando efeitos tanto moluscicidas como e ovicidas, nos testes realizados o que a credencia, para a utilização em ensaios de campo, contra moluscos da espécie L. columella, hospedeiros intermediários para o parasito Fasciola hepatica.

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Aspectos histopatológicos de Biomphalaria glabrata Say, 1817, hospedeiro intermediário de Schistosoma mansoni, submetida a Niclosamida

Cited by 4 publications

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Destruction of secondary Schistosoma mansoni sporocysts in Biomphalaria glabrata after phytochemical exposure

Destruction of secondary Schistosoma mansoni sporocysts in Biomphalaria glabrata after phytochemical exposure

Physiological changes in Biomphalaria glabrata say, 1818 (Pulmonata: Planorbidae) caused by sub-lethal concentrations of the latex of Euphorbia splendens var. hislopii N.E.B (Euphorbiaceae)

Plantas Do Cerrado Brasileiro Como Possíveis Agentes Moluscicidas

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