Isolation of Mycobacterium spp. in milk from cows suspected or positive to tuberculosis

Pardo, Renata; Langoni, Hélio; Mendonça, Lia Jeanne Pereira; Dahr, Kung

doi:10.1590/s1413-95962001000600007

Cited by 30 publications

(27 citation statements)

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“…In Turkey, 35 samples of raw milk were evaluated for the presence of mycobacteria using phenotypic methods and confirmed by Polymerase Chain Reaction - Restriction Pattern Analysis (PCR-PRA), resulting in the detection of M. terrae, M. kansassii, M. agri, and M. haemophilum 32 . In another study in the same country, M. genavense, M. simiae, M. szulgai, M. bovis and M. fortuitum 61 were identified .…”

Section: Mycobacteria In Dairy Cattlementioning

confidence: 99%

Diagnosis of mycobacteria in bovine milk: an overview

Bolaños

Paula

Guerra

et al. 2017

Rev. Inst. Med. trop. S. Paulo

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Tuberculosis remains as the world’s biggest threat. In 2014, human tuberculosis ranked as a major infectious disease by the first time, overcoming HIV death rates. Bovine tuberculosis is a chronic disease of global distribution that affects animals and can be transmitted to humans by the consumption of raw milk, representing a serious public health concern. Despite the efforts of different countries to control and eradicate bovine tuberculosis, the high negative economic impact on meat and milk production chains remains, given the decreased production efficiency (approximately 25%), the high number of condemned carcasses, and increased animal culling rates. This scenario has motivated the establishment of official programs based on regulations and diagnostic procedures. Although Mycobacterium tuberculosis and Mycobacterium bovis are the major pathogenic species to humans and bovines, respectively, nontuberculous mycobacteria within the Mycobacterium genus have become increasingly important in recent decades due to human infections, including the ones that occur in immunocompetent people. Diagnosis of mycobacteria can be performed by microbiological culture from tissue samples (lymph nodes, lungs) and secretions (sputum, milk). In general, these pathogens demand special nutrient requirements for isolation/growth, and the use of selective and rich culture media. Indeed, within these genera, mycobacteria are classified as either fast- or slow-growth microorganisms. Regarding the latter ones, incubation times can vary from 45 to 90 days. Although microbiological culture is still considered the gold standard method for diagnosis, molecular approaches have been increasingly used. We describe here an overview of the diagnosis of Mycobacterium species in bovine milk.

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Section: Mycobacteria In Dairy Cattlementioning

confidence: 99%

Diagnosis of mycobacteria in bovine milk: an overview

Bolaños

Paula

Guerra

et al. 2017

Rev. Inst. Med. trop. S. Paulo

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“…NTM species may be transmitted to humans from the environment, such as ingestion of contaminated food (Pardo et al , 2001), water, fruit, vegetable and milk (Konuk et al , 2007). There is no evidence that these organisms are transmitted person-to-person (Schlossberg, 2006).…”

Section: Introductionmentioning

confidence: 99%

Occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk in the northwestern region of Paraná, Brazil

Sgarioni

Hirata

et al. 2014

Braz. J. Microbiol.

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Milk is widely consumed in Brazil and can be the vehicle of agent transmission. In this study, was evaluated the occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk consumed in the northwestern region of Paraná, Brazil. Fifty-two milk samples (20 pasteurized and 32 raw) from dairy farms near the municipality of Maringa, Parana State, Brazil were collected. Milk samples were decontaminated using 5% oxalic acid method and cultured on Lowenstein-Jensen and Stonebrink media at 35 °C and 30 °C, with and without 5–10% CO2. Mycobacteria isolates were identified by morphological features, PCR-Restriction Fragment Length Polymorphism Analysis (PCR-PRA) and Mycolic acids analysis. Thirteen (25%) raw and 2 (4%) pasteurized milk samples were positive for acid fast bacilli growth. Nine different species of NTM were isolated (M. nonchromogenicum, M. peregrinum, M. smegmatis, M. neoaurum, M. fortuitum, M. chelonae, M. flavescens, M. kansasii and M. scrofulaceum). M. bovis was not detected. Raw and pasteurized milk may be considered one source for NTM human infection. The paper reinforces the need for intensification of measures in order to avoid the milk contamination and consequently prevent diseases in the south of Brazil.

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“…Several studies have been conducted in other states in order to isolate and identify mycobacteria in milk from cows. In São Paulo, PARDO et al (2001) isolated M. bovis in one sample of raw milk; LEITE et al (2003), using milk samples from the states of São Paulo, Paraná, Santa Catarina, Goiás and Pará, isolated M. bovis in one sample of raw milk. FRANCO et al (2013), in the southeast region of São Paulo, isolated M. bovis from one milk sample from an individual cooling tank.…”

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confidence: 99%