Evaluation of the automated system Vitek2 for identification and antimicrobial susceptibility testing of Brazilian Gram-positive cocci strains

Abstract

Automated instruments offer many advantages for clinical laboratories. Nevertheless, they can have problems identifying and determining susceptibilities of some pathogens. Vitek ® 2 (bioMérieux) is an automated system that was recently introduced to Brazil. We evaluated the performance of this equipment for Brazilian isolates that had been characterized using reference identification and antimicrobial susceptibility testing methods. Ninety-nine strains of Gram-positive cocci from a local reference center collection were analyzed, consisting of 50 coagulasenegative Staphylococcus (

Bacteremias por Staphylococcus coagulase negativos oxacilina resistentes em um hospital escola na cidade de Santa Maria, Estado do Rio Grande do Sul

“…Apesar das vantagens oferecidas por este sistema, tais como, logística no fluxo de trabalho e rapidez no fornecimento de resultados, ele pode apresentar limitações na identificação e determinação da suscetibilidade de alguns patógenos 26 .…”

“…Devido à emergência de infecções nosocomiais associadas a SCN, a identificação das cepas em nível de espécie torna-se um fator importante, a fim de definir o seu significado clínico e realizar estudos epidemiológicos adequados 26 . O percentual de significância clínica dos nossos isolados foi superior ao descrito na literatura 1 .…”

Coagulase-negative staphylococci in Southern Brazil: looking toward its high diversity

“…(22) MDR was defined as a resistant phenotype to at least one agent in three or more antimicrobial categories (23) .…”

The use of whole‐cell protein profile analysis by SDS‐PAGE as an accurate tool to identify species and subspecies of coagulase‐negative staphylococci

“…Although several techniques have been used to identify CoNS, including phenotypic and genotypic methods, not all can reliably identify less frequent isolates, for which accurate and precise identification is strongly needed (4, 5). The phenotypic characterization of CoNS was performed using conventional phenotypic tests and automated systems (6).…”