A study of iga antibody response to different mycobacterium tuberculosis antigens in the diagnosis and monitoring of pulmonary tuberculosis

Bezerra, Janaína Miranda; Beck, Sandra Trevisan; Kanunfre, Kelly Aparecida; Leite, Olavo Munhoz; Ferreira, Antônio Walter

doi:10.1590/s1413-86702009000100012

Cited by 14 publications

(23 citation statements)

References 23 publications

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“…IgA is the most abundantly produced mammalian immunoglobulin, and although IgA-secreting plasma cells are concentrated along mucous membrane surfaces, it is produced in the pleural space, since the pleura is a serous membrane (28,41). On the other hand, several authors have pointed to the presence of IgA against the mycobacterial antigens in the serum of TB patients (3,11,33,45). Regrettably, in our study, serum samples of patients were not available for a matching analysis.…”

Section: Discussionmentioning

confidence: 78%

Assessment of the IgA Immunoassay Diagnostic Potential of the Mycobacterium tuberculosis MT10.3-MPT64 Fusion Protein in Tuberculous Pleural Fluid

Araujo

Maciel

Trajman

et al. 2010

Clin Vaccine Immunol

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Pleural tuberculosis (PL-TB) remains difficult to diagnose. An enzyme-linked immunosorbent assay (ELISA) was developed based on a construction containing the fusion of the Rv3019c (MT10.3) and Rv1980c (MPT64) gene sequences, and its performance was evaluated in an area where TB is endemic. A total of 92 pleural fluid (PF) samples at serial dilutions of 1:50 to 1:800 were included in the ELISA IgA MT10. 4%). Our findings demonstrated the promising use of this test as an adjunct in PL-TB diagnoses, particularly in cases with lower bacterial loads and false-negative results in the reference tests, since the new test includes such important features as quick and easy application, high sensitivity and, perhaps most importantly, affordability, which is so crucial for its widespread use in developing countries.Tuberculosis (TB) is the leading cause of preventable morbidity and mortality from infectious agents worldwide. A global plan has been outlined to eradicate the disease by 2050 (37). To reach this goal, both political commitments and increments in interdisciplinary research are needed; because the specific diagnostics, drugs, and vaccines available for this purpose have been insufficient in eradicating the disease, the development of more effective tools and strategies is urgently needed (7).In Brazil, the TB prevalence rate is approximately 60 per 100,000 inhabitants (44). Generally speaking, the incidence of pleural tuberculosis (PL-TB) is closely related to the local disease prevalence. Moreover, PL-TB is the major cause of pleural effusions, responsible for approximately 50% of all related diagnoses in Brazil (25, 32).TB diagnoses have traditionally relied on the identification of acid-fast bacilli (AFB) and the culture of clinical specimens. However, low sensitivities (5 and 47%, respectively) are achieved with PL-TB. Besides, the final culture results may take as long as 4 to 6 weeks to complete, delaying the initiation of specific therapy.The histopathological examination of pleural biopsy specimens comprises the standard PL-TB reference test. Nevertheless, its sensitivity is highly variable (39 to 84%), it is expensive and time-consuming, and its proper administration requires skilled personnel. It is clear that there is an overriding need for the development of a simple, fast, effective, and affordable yet robust tool that would be readily available throughout the national public health care sector to aid in the diagnosis of PL-TB, with special emphasis on developing countries (12,15,30). What is most frequently used is an enzyme-linked immunosorbent assay (ELISA) together with commercial and inhouse antibody tests, which have been developed using antigens in a cocktail-like format. The downside is that lower reactivity has been described for this format compared with the rates obtained after testing these components individually (9,13,22). In our previous studies, when the single proteins MPT64 and MT10.3 were tested individually for each patient, the combined results achieved a 76% sensitivity rate in...

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Section: Discussionmentioning

confidence: 78%

Assessment of the IgA Immunoassay Diagnostic Potential of the Mycobacterium tuberculosis MT10.3-MPT64 Fusion Protein in Tuberculous Pleural Fluid

Araujo

Maciel

Trajman

et al. 2010

Clin Vaccine Immunol

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“…This could be an additional evidence for the potential of IgA antibody in the development of serological diagnostic tools for latent TB [40,44], which warrants further studies like in household contacts of patients with smear-positive PTB.…”

Section: Discussionmentioning

confidence: 79%

“…Conde et al [38] suggested that IgA antibody against P-90 antigen can distinguish individuals recently infected with Mtb. Arikan et al [39] and Bezerra et al [40] have evaluated the performance of ELISA-based IgA antibody for the diagnosis of active TB using different Mtb antigens and suggested that serum IgA antibody has a promising role in the diagnosis of active TB. Skvor et al [41] have reported an increased level of IgA in relation to the extent of disease in patients with PTB.…”

Section: Discussionmentioning

confidence: 99%

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IgA Response to ESAT‐6/CFP‐10 and Rv2031 Antigens Varies in Patients With Culture‐Confirmed Pulmonary Tuberculosis, Healthy Mycobacterium tuberculosis–Infected and Non‐Infected Individuals in a Tuberculosis Endemic Setting, Ethiopia

et al. 2013

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Little attention has been given to the role of antibodies against Mycobacterium tuberculosis (Mtb) infection. We have compared the levels of IgA and IgG against ESAT-6/CFP-10 and Rv2031c antigens in sera of patients with cultureconfirmed pulmonary tuberculosis (PTB), healthy Mtb-infected and non-infected individuals in endemic TB settings. Venous blood samples were collected from 166 study participants; sera were separated and assayed by an enzyme-linked immunosorbent assay (ELISA). QuantiFERON-TB Gold In-Tube (QFTGIT) assay was used for the screening of latent TB infection. The mean optical density (OD) values of IgA against ESAT-6/CFP-10 and Rv2031 were significantly higher in sera of patients with culture-confirmed PTB compared with healthy Mtb-infected and non-infected individuals (P < 0.001). The mean OD values of IgG against ESAT-6/CFP-10 and Rv2031 were also significantly higher in sera of patients with culture-confirmed PTB compared with healthy Mtb-infected and non-infected individuals (P < 0.05). The mean OD values of IgA against both antigens were also higher in sera of healthy Mtb-infected cases compared with non-infected individuals. There were positive correlations (P < 0.05) between the level of IFN-c induced in QFTGIT assay and the OD values of serum IgA against both antigens in healthy Mtb-infected subjects. This study shows the potential of IgA response against ESAT-6/CFP-10 and Rv2031 antigens in discriminating clinical TB from healthy Mtb-infected and non-infected cases. Nevertheless, further well-designed cohort study is needed to fully realize the full potential of this diagnostic marker.

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“…Several authors have described the presence of IgA against the mycobacterial antigens in the serum of TB patients [26,28-31], suggesting that this immunoglobulin isotype is present in both patients with newly acquired infections and those of a longer duration [32]. In the present investigation, the positive rate of IgA was not too high in active TB patients at their first visit to the outpatient clinic and the IgA antibody levels were maintained for a longer duration until the completion of anti-TB chemotherapy.…”

Section: Discussionmentioning

confidence: 99%

IgG, IgM and IgA antibodies against the novel polyprotein in active tuberculosis

Xia¹,

Yang²,

Xiu³

et al. 2014

BMC Infect Dis

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BackgroundThe present study was aimed to evaluate whether IgG, IgM and IgA antibodies levels detected against a novel Mycobacterium tuberculosis polyprotein 38 F-64 F (with 38 F being the abbreviation for 38kD-ESAT6-CFP10 and 64 F for Mtb8.4-MPT64-TB16.3-Mtb8) are suitable for diagnosing active tuberculosis, and for monitoring the efficacy of chemotherapy on TB patients.MethodsIn this study, a total of 371 active TB patients without treatment were selected and categorized into S+/C+ group (n = 143), S-/C+ group (n = 106) or S-/C- group (n = 122). A series of serum samples were collected from 82 active TB patients who had undergone anti-TB chemotherapy for 0–6 months at one month interval. Humoral responses (IgG, IgM and IgA) were determined for the novel Mycobacterium tuberculosis polyprotein using indirect ELISA methods in all of serum samples.ResultsFor S+/C+, S-/C+ and S-/C- active tuberculosis patients before anti-TB chemotherapy, the sensitivities of tests based on IgG were 65.7%, 46.2% and 52.5% respectively; the sensitivities based on IgM were 21.7%, 24.5% and 18.9%; and the sensitivities based on IgA were 25.2%, 17.9% and 23.8%. By combination of three isotypes, for all active tuberculosis patients, the test sensitivity increased to 70.4% with the specificity being 91.5%. After anti-TB chemotherapy, there were no significant differences between groups with different courses of anti-TB chemotherapy.ConclusionsThe novel Mycobacterium tuberculosis polyprotein 38 F-64 F represents potential antigen suitable for measuring IgG, IgM and IgA antibodies. However, the serodiagnostic test based on the 38 F-64 F polyprotein appears unsuitable for monitoring the efficacy of chemotherapy.

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A study of iga antibody response to different mycobacterium tuberculosis antigens in the diagnosis and monitoring of pulmonary tuberculosis

Cited by 14 publications

References 23 publications

Assessment of the IgA Immunoassay Diagnostic Potential of the Mycobacterium tuberculosis MT10.3-MPT64 Fusion Protein in Tuberculous Pleural Fluid

Assessment of the IgA Immunoassay Diagnostic Potential of the Mycobacterium tuberculosis MT10.3-MPT64 Fusion Protein in Tuberculous Pleural Fluid

IgA Response to ESAT‐6/CFP‐10 and Rv2031 Antigens Varies in Patients With Culture‐Confirmed Pulmonary Tuberculosis, Healthy Mycobacterium tuberculosis–Infected and Non‐Infected Individuals in a Tuberculosis Endemic Setting, Ethiopia

IgG, IgM and IgA antibodies against the novel polyprotein in active tuberculosis

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