Comparison of PCR-based molecular markers for the characterization of Proteus mirabilis clinical isolates

Abstract

Proteus mirabilis is one of the most important pathogens associated with complicated urinary tract infections (acute pyelonephritis, bladder infections, kidney stones) and bacteremia, affecting patients with anatomical abnormalities, immunodeficiency, and long-term urinary catheterization. For epidemiological purposes, various molecular typing methods, such as pulse-field gel electrophoresis (PFGE) or ribotyping, have been developed for this pathogen. However, these methods are labor intensive and time-consuming. We evaluated the discriminatory power of several PCR-based fingerprinting methods (RAPD, ISSR, ERIC-PCR, BOX-PCR and rep-PCR) for P. mirabilis clinical isolates. Typing patterns and clustering analysis indicated that RAPD, BOX-PCR and ERIC-PCR differentiated P. mirabilis strains from Escherichia coli, Hafnia alvei, and Morganella morganii. With the exception of rep-PCR, the methods gave medium to high discriminatory efficiency in P. mirabilis. In general, the results obtained with RAPD, BOX-PCR and ERIC-PCR were in good agreement. We concluded that a combination of ERIC-PCR and BOX-PCR results is a rapid and reliable alternative for discrimination among P. mirabilis clinical isolates, contributing to epidemiological studies.

Identification, antibiotic resistance and distribution of different classes of integrons among proteus species isolated from different sources in Dakahleia and Damietta Egyptian Governorates

“…Such studies conforms with those of Michelin et al (2008), who worked on 35 strains from different hospitals. The authors concluded that the only epidemiologically related strains were those strain isolated from one and the same patient occurring few days after endogenous infection of the patient himself.…”

The Sequencing of hpmB Gene in Proteus mirabilis Among UTIs Patients

“…Results indicate that since there is an increase in clinical significance of P. mirabilis, the choice of effectual molecular methods is of great epidemiological reputation. Bacterial genotyping opened new chances on epidemiological studies by the documentation of clinical and ecological isolates, the assessment of this association, the watching of clone propagation and the classification of bacterial populations within more or less constrained environments 24 . By joining molecular phylogeny with traditional approach such as morphological, physiological and biochemical characteristics, bacteria identification could be achieved in a more accurate way 25,26 .…”

Differentiation ofClavibacter michiganensissubsp.sepedonicususing PCR melting profile and variable number of tandem repeat methods

“…The molecular typing of Cms was previously performed using universal PCR‐based methods such as RAPD, BOX‐PCR and ERIC–PCR, which were successfully used in a differentiation of a number of bacterial species including C.m.michiganensis, C.m.insidious and C.m.nebraskensis (Pastrik ; Michelim et al . ; Singh and Tripathi ). In the case of Cms, a low level of genetic variability was observed (Mogen et al .…”