Phenotypical characteristics of group B streptococcus in parturients

Simões, J. A.; Alves, Valéria Moraes Neder; Fracalanzza, Sérgio Eduardo Longo; Camargo, Rodrigo Paupério Soares de; Mathias, L; Milanez, Helaine; Brolazo, Eliane Melo

doi:10.1590/s1413-86702007000200019

Cited by 33 publications

(40 citation statements)

References 34 publications

(34 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Duarte et al (2005) described some resistance to erythromycin (5%) among Ia strains collected between 2000-2001 in Rio de Janeiro. In the present report, susceptibility to other antimicrobial agents is in agreement with other data in the literature (Hsueh et al 2001, Uh et al 2001, Simões et al 2007.…”

Section: Resultssupporting

confidence: 93%

See 1 more Smart Citation

Pulsed-field gel electrophoresis, virulence determinants and antimicrobial susceptibility profiles of type Ia group B streptococci isolated from humans in Brazil

Corrêa

Oliveira

Pinto

et al. 2009

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

Group B streptococci (GBS) infections occur worldwide. Although serotyping has been used for epidemiologic purposes, this does not accurately characterize enough members of a genetically heterogeneous bacterial population. The aims of this work were to evaluate the genetic diversity of 45 type Ia GBS strains isolated in Brazil byGroup B streptococci (GBS) are responsible for a large variety of human infections and have been recognized over the last few decades as a leading cause of perinatal disease worldwide (Farley 2001, Weisner et al. 2004. GBS are classified in serotypes (Ia, Ib and II-VIII) that occur in combination with different protein antigens (alpha, beta and rib) (Dmitriev et al. 2001). Currently, serotypes Ia, III and V are the most common in many countries (Duarte et al. 2005, Fluegge et al. 2005, Bergseng et al. 2008, Poyart et al. 2008, von Both et al. 2008.Pulsed-field gel electrophoresis (PFGE), used to examine GBS strains, is a powerful technique employed for the classification of microorganisms after digestion of the genomic DNA by restriction enzymes (Benson & Ferrieri 2001, Oliveira et al. 2005, von Both et al. 2008. Using polymerase chain reaction (PCR), Franken et al. (2001) suggested that the scpB and lmb genes (encoding c5a peptidase and laminin binding protein) must exist in GBS strains that infect humans. The hylB gene is frequently detected in these strains (Dore et al. 2003). The bac and bca genes are present in 23% and 100% of type Ia strains, respectively (Maeland et al. 1997(Maeland et al. , 2000.The lack of information on Ia Brazilian strains and the availability of DNA techniques led us to investigate the genetic make-up of type Ia GBS strains isolated in distinct regions of Brazil. Additionally, antimicrobial susceptibility was examined to better characterize these isolates. MATERIALS AND METHODSBacterial strains -Forty-five human type Ia GBS strains derived from clinical specimens were obtained in Florianopolis, Santa Catarina (n = 3), a city located in the Southern Region of Brazil, in São Paulo, São Paulo (SP) (n = 1), and in Rio de Janeiro, Rio de Janeiro (n = 40), in the Southeast Region of the country. The source of one strain was unknown.Isolates were obtained from 1981-2002 from public health laboratories, gynaecological clinics, hospitalsand universities. The clinical sources included urine (n = 16), oropharynx (n = 9), vagina (n = 5), anus (n = 2), lung (n = 2), placenta (n = 1), external ear canal (n = 1) and perineum (n = 1). The sources of eight specimens were unknown. A confirmatory identification of serotypes was carried out again by immunoprecipitation in agarose using sera produced in the research facilities of the authors and HCl antigenic extracts from the streptococci (Benchetrit et al. 1982).PFGE -PFGE was performed as previously described (Oliveira et al. 2005). DNA was digested with the SmaI restriction enzyme (Amersham) and submitted to electrophoresis with a program as follows: switch time of 1-30 sec during 23 h, with a 120º angle, at a temperature of ...

show abstract

Section: Resultssupporting

confidence: 93%

“…Simões et al (2007) did not detect resistance to erythromycin and clindamycin among Ia isolates collected between 2003-2004 in São Paulo. We also did not detect resistance to these two antibiotics.…”

Section: Resultsmentioning

confidence: 70%

Pulsed-field gel electrophoresis, virulence determinants and antimicrobial susceptibility profiles of type Ia group B streptococci isolated from humans in Brazil

Corrêa

Oliveira

Pinto

et al. 2009

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

show abstract

“…This worldwide variability is related to different sociocultural, geographic, climatic, biological and methodological determinants. Brazilian studies have found rates ranging from 5 to 25% (3,5,22,23,26,29,30). Our finding of a 32.6% rate of GBS prevalence using the genetic detection directly from the enrichment culture is worrisome and the highest reported in the country to our knowledge so far.…”

Section: Discussionmentioning

confidence: 99%

“…Brazilian authors have found colonization rates from 5% to 25% in regional studies (3,22,23,26,29,30). GBS colonization may be transient, chronic or intermittent (28).…”

Section: Lancefield Group B Streptococcus (Gbs) Ormentioning

confidence: 99%

Detection of Group B Streptococcus in Brazilian pregnant women and antimicrobial susceptibility patterns

Castellano-Filho¹,

Silva²,

Nascimento³

et al. 2010

Braz. J. Microbiol.

View full text Add to dashboard Cite

Group B Streptococcus (GBS) is still not routinely screened during pregnancy in Brazil, being prophylaxis and empirical treatment based on identification of risk groups. This study aimed to investigate GBS prevalence in Brazilian pregnant women by culture or polymerase chain reaction (PCR) associated to the enrichment culture, and to determine the antimicrobial susceptibility patterns of isolated bacteria, so as to support public health policies and empirical prophylaxis. After an epidemiological survey, vaginal and anorectal specimens were collected from 221 consenting laboring women. Each sample was submitted to enrichment culture and sheep blood agar was used to isolate suggestive GBS. Alternatively, specific PCR was performed from enrichment cultures. Antimicrobial susceptibility patterns were determined for isolated bacteria by agar diffusion method. No risk groups were identified. Considering the culture-based methodology, GBS was detected in 9.5% of the donors. Twenty five bacterial strains were isolated and identified. Through the culture-PCR methodology, GBS was detected in 32.6% specimens. Bacterial resistance was not detected against ampicillin, cephazolin, vancomycin and ciprofloxacin, whereas 22.7% were resistant to erythromycin and 50% were resistant to clindamycin. GBS detection may be improved by the association of PCR and enrichment culture. Considering that colony selection in agar plates may be laboring and technician-dependent, it may not reflect the real prevalence of streptococci. As in Brazil prevention strategies to reduce the GBS associated diseases have not been adopted, prospective studies are needed to anchor public health policies especially considering the regional GBS antimicrobial susceptibility patterns.

show abstract

“…This colonization is a dynamic condition and represents the main risk factor for early neonatal infection. Notably, the international literature reports maternal GBS colonization rates of 6.5-36.0% in Europe 6,7 , 10.0-30.0% in North America 2,8 , 16.5-31.6% in African countries 9 , and 1.4-36.7% in South America, including Brazil [10][11][12][13] , Chile 14 , Peru 15 , and Argentina 16 .…”

Section: Introductionmentioning

confidence: 99%

Group B Streptococcus detection in pregnant women via culture and PCR methods

Wollheim

Sperhacke

Fontana

et al. 2017

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

Introduction: Group B Streptococcus (GBS), a source of neonatal infection, colonizes the gastrointestinal and genitourinary tracts of pregnant women. Routine screening for maternal GBS in late pregnancy and consequent intrapartum antibiotic prophylaxis have reduced the incidence of early-onset GBS neonatal infection. The aim of this study was to evaluate the performance of PCR, compared to culture (gold standard), in GBS colonization screening of pregnant women, and to establish the prevalence of GBS colonization among this population. Methods: Vaginal introitus and perianal samples were collected from 204 pregnant women, between the 35 th and 37 th weeks of pregnancy, at the Obstetrics and Gynecology Unit of the University of Caxias do Sul General Hospital between June 2008 and September 2009. All samples were cultured after enrichment in a selective medium and then assayed by culture and PCR methods. Results: The culture and PCR methods yielded detection rates of vaginal/perianal GBS colonization of 22.5% and 26%, respectively (sensitivity 100%; specificity 95.6%; positive and negative predictive values 86.8% and 100%, respectively). A higher prevalence of GBS colonization was detected in the combined vaginal and perianal samples by both culture and PCR assay analyses. Conclusions: PCR is a faster and more efficient method for GBS screening, allowing for optimal identification of women who should receive intrapartum antibiotic prophylaxis to prevent newborn infection.

show abstract

Phenotypical characteristics of group B streptococcus in parturients

Cited by 33 publications

References 34 publications

Pulsed-field gel electrophoresis, virulence determinants and antimicrobial susceptibility profiles of type Ia group B streptococci isolated from humans in Brazil

Pulsed-field gel electrophoresis, virulence determinants and antimicrobial susceptibility profiles of type Ia group B streptococci isolated from humans in Brazil

Detection of Group B Streptococcus in Brazilian pregnant women and antimicrobial susceptibility patterns

Group B Streptococcus detection in pregnant women via culture and PCR methods

Contact Info

Product

Resources

About