Fatal bacteremia due to Anaerobiospirillum succiniciproducens: first description in Brazil

Secchi, Carina; Cantarelli, Vlademir Vicente; Pereira, Fabiana de Souza; Wolf, Hilda Helena Chaer; Brodt, Teresa; Amaro, Maria Cristina O.; Inamine, Everton

doi:10.1590/s1413-86702005000200008

Cited by 16 publications

(5 citation statements)

References 12 publications

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“…(20). However, the identity of A. succiniciproducens has been confirmed with a 16S rRNA sequencing test in only 6 cases (2,12,18,20).…”

Section: Case Reportmentioning

confidence: 97%

“…In all of these cases treatment was empirical and A. succiniciproducens was identified only later during hospitalization, as in our case. Duration of antimicrobial therapy was reported in only 5 cases and varied between 7 and 14 days (10,16,18,20,22). Thus, further studies are needed to define the role of antimicrobial therapy in BSI with A. succiniciproducens.…”

Section: Case Reportmentioning

confidence: 99%

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First Report of Treatment of Anaerobiospirillum s ucciniciproducens Bloodstream Infection with Levofloxacin

et al. 2010

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The full extent of the clinical spectrum and optimal therapy of Anaerobiospirillum succiniciproducens infections remains to be determined. We describe the first case of bloodstream infection (BSI) due to A. succiniciproducens in an asymptomatic elderly male with poor dentition that was treated with levofloxacin. CASE REPORTAn 81-year-old male presented to our institution for evaluation and treatment of worsening depression. His past medical history was significant for squamous cell carcinoma of the right cheek, for which he had undergone surgery and radiation therapy 15 years ago. He had a nonproductive cough for 3 months, but he denied fever, chills, nausea, vomiting, abdominal pain, or diarrhea. He had not seen a dentist for many years, and he denied any exposure to any pets, including cats or dogs. Physical examination revealed normal temperature, poor dentition, and rales at the right lung base but was otherwise unremarkable. His white blood cell count was 11,500 cells/mm 3 with 82% polymorphonuclear cells, and other laboratory data were normal. A chest X ray revealed an infiltrate at the right lung base, and the patient was started on oral levofloxacin, 500 mg daily for 10 days, for empirical treatment of community-acquired pneumonia. Blood cultures (the anaerobic bottles from 2 sets of blood cultures) obtained on admission due to his pneumonia subsequently grew a spiral Gram-negative rod after 4 days. This was ultimately identified as Anaerobiospirillum succiniciproducens.Bacterial growth was detected in two anaerobic bottles on day 4 of incubation using the BacT/Alert (bioMérieux, Marcy l'Etoile, France). Large Gram-negative spiral-shaped bacteria, measuring on average 6 m in length by 0.7 m in width, were detected on Gram stain (Fig. 1), and corkscrew motility was noted on wet mount. The organism was subcultured to blood agar, chocolate blood agar, and thioglycolate broth; incubated at 35°C in 5% CO 2 and brucella blood agar; and incubated at 35°C under anaerobic conditions. To rule out Campylobacter species, the organism was also subcultured to brucella blood agar and incubated at 35°C and 42°C in a microaerophilic environment. Anaerobic growth on brucella blood agar revealed translucent, nonhemolytic colonies; no growth was observed on blood agar or brucella blood agar in a microaerophilic atmosphere. The isolate was oxidase, catalase, and nitrate negative. RapID ANA II (Remel, Lenexa, KS) revealed positive ␣-glucosidase and N-acetylglucosaminidase reactions but was unable to successfully identify the organism. We further confirmed the identification of Anaerobiospirillum spp. by investigating the specific flagellar arrangement using a transmission electron microscope. Spiral bacteria with bipolar multitrichous flagella were evident in all grid fields examined (Fig. 2).Molecular identification of the bacterium was obtained by sequencing of the 16S rRNA gene. Whole-genomic DNA was extracted from a single colony of the bacterium using the NucliSENS easyMag system (bioMérieux, Durham, NC). The 5Ј 527 base...

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“…(20). However, the identity of A. succiniciproducens has been confirmed with a 16S rRNA sequencing test in only 6 cases (2,12,18,20).…”

Section: Case Reportmentioning

confidence: 97%

Section: Case Reportmentioning

confidence: 99%

First Report of Treatment of Anaerobiospirillum s ucciniciproducens Bloodstream Infection with Levofloxacin

et al. 2010

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“…Reported clinical outcomes associated with A. succiniciproducens infection range from rapid resolution to progressive illness and death. 16 However, growth only in the aerobic bottle would be highly atypical; aerobic growth has been reported in just one instance 18 and not without accompanying growth in the anaerobic bottle.…”

Section: Corkscrew-shaped Gram-negative Bacteremiamentioning

confidence: 99%

Case 30-2017

2017

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A 65-year-old woman with autoimmune hepatitis and acute liver failure was transferred to this hospital for an evaluation for liver transplantation. The patient had been in her usual state of health until 6 years before this admission, when she received abnormal results of liver-function tests at another hospital. She underwent a follow-up evaluation that revealed an elevated antinuclear antibody titer. Levels of ceruloplasmin, α 1-antitrypsin, and antimitochondrial antibodies were normal, and tests for tissue transglutaminase antibodies and viral hepatitis were negative. A presumptive diagnosis of autoimmune hepatitis with cirrhosis was made, and treatment with glucocorticoids was recommended. However, results of liver-function tests reportedly normalized without treatment during the following year. Approximately 7 weeks before this admission, malaise, fatigue, and jaundice developed in the patient, and she was evaluated at the other hospital. The alanine aminotransferase and aspartate aminotransferase levels were elevated at 1844 IU per liter (reference range, 0 to 40) and 1451 IU per liter (reference range, 0 to 37), respectively. She had a bilirubin level of 7.9 mg per deciliter (135.1 μmol per liter; reference range, 0 to 1.0 mg per deciliter [0 to 17.1 μmol per liter]), an antinuclear antibody titer of 1:640 (reference range, <1:160), detectable anti-smooth muscle antibodies, and an IgG level of 2584 mg per deciliter (reference range, 700 to 1600). Ultrasonography of the right upper quadrant revealed a nodular liver. Prednisone therapy was begun. The patient's condition improved, and plans were made to transition treatment from prednisone to azathioprine. However, the patient elected to taper the course of prednisone within 4 weeks and started to take natural supplements instead of the prescribed azathioprine. Two weeks later, approximately 7 days before this admission, the patient's daughter noted that her mother had jaundice. Four days before this admission, the

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“…Anaerobiospirillum members are part of the normal gastrointestinal microbiota of companion animals (dogs and cats) and are suspected to be the source of bacteraemia, gastrointestinal infections, and pyomyositis in humans (Decroix et al, 2016;Epstein et al, 2017;Fadzilah et al, 2009;Inokuchi et al, 2014;Pienaar et al, 2003;Schaumburg et al, 2017;Secchi et al, 2005). Zoonotic transmission of Anaerobiospirillum sp.…”

Section: F I G U R Ementioning

confidence: 99%

Exploring the role of healthy dogs as hosts of enterohepatic Helicobacter species using cultivation‐dependent and ‐independent approaches

Ochoa

Ojeda

Martínez

et al. 2021

Zoonoses and Public Health

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Enterohepatic Helicobacter (EHH) species have been increasingly associated with acute gastroenteritis, inflammatory bowel disease and hepatobiliary diseases in humans. However, their host range and transmission routes are poorly understood.Therefore, the aim of this study was to determine the presence of EHH in healthy dogs using both cultivation-dependent and -independent methods. Three hundred and ninety faecal samples from domestic dogs without gastrointestinal symptoms were analysed between June 2018 and July 2019 in Valdivia (South of Chile). Samples were inoculated on selective medium and in parallel were filtrated over an antibioticfree blood agar. Both media were incubated in a microaerobic atmosphere at 37°C for 7 days. Colonies were identified by PCR and phylogenetic analysis. A subset of 50 samples (half of them positive for EHH by cultivation and the remaining half negative) was analysed by PCR-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) for direct detection. Cultivation method detected EHH in 15.4% (60/390) of the samples, being the most prevalent species H. canis (5.8%, 23/390) and H. canicola (5.1%, 20/390), followed by H. bilis (3.6%, 14/390) and 'H. winghamensis' (1.3%, 5/390).In contrast, PCR-DGGE method detected Helicobacter DNA in almost all (96%, 48/50) tested samples. On the other hand, the method used also allowed to isolate other Campylobacterales, in fact 44.3% (173/390) of the samples were positive for Campylobacter upsaliensis (43.3%, 169/390) followed by C. jejuni (2.0%, 8/390). Moreover, two strains that presented Campylobacter-like morphology were finally identified as Anaerobiospirillum succiniciproducens. Our results indicate that healthy domestic dogs commonly carry EHH and other Campylobacter species. However, further studies are needed to determine whether and how these Helicobacter and Campylobacter species can be transmitted to humans.

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Fatal bacteremia due to Anaerobiospirillum succiniciproducens: first description in Brazil

Cited by 16 publications

References 12 publications

First Report of Treatment of Anaerobiospirillum s ucciniciproducens Bloodstream Infection with Levofloxacin

First Report of Treatment of Anaerobiospirillum s ucciniciproducens Bloodstream Infection with Levofloxacin

Case 30-2017

Exploring the role of healthy dogs as hosts of enterohepatic Helicobacter species using cultivation‐dependent and ‐independent approaches

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