Desenvolvimento de um modelo experimental de falha óssea infectada na ulna de coelhos

Abstract

ObjectiveDevelop a model that allowed the study of bone regeneration in infection conditions.MethodA 15 mm defect was surgically created in the rabbit ulna and inoculated with 5x108 colony-forming units (CFU) of S. aureus. Surgical debridement was performed two weeks after and systemic gentamicin was administered for four weeks. Animals were followed up to 12 weeks to evaluate infection control and bone regeneration.ResultBone regeneration was inferior to 25% of the defect in radiological and histological analysis.ConclusionInfected bone defect of 15 mm in the rabbit ulna was unable to achieve full regeneration without further treatment. Level of Evidence V, Experimental Study.

A Critical Size Defect Model in the Radius of Rabbits

“…The total time of the experimental phase to confirm a defect non-union is dependent on several factors, however it is short when using rabbits (AZI et al, 2012;DEN BOER et al, 1999), once they have fast bone turnover, allowing the study to be performed in a shorter period when compared to other species (CASTAÑEDA et al, 2006). Additionally, young adults were selected, facilitating and standardizing the time of achieving CSD.…”

“…Different methodologies have been proposed for achieving an ideal CSD. Some of them consider the total size of the gap, and others prefer to use a proportion of the total length or width of the bone (BOLANDER; BALIAN, 1986;GOGOLEWSKI, 2007;ZHAO et al, 2016). Spontaneously healing was detected in defects with 1,0 cm and 1,2 cm in radius of rabbits, however other study proved that no spontaneously bone healing can occur when the defect size has 1 cm and 1,2 cm, and above 1,4 cm the bone growth was significant lesser (ZHAO et al, 2016).…”

“…In this study, the defect size was anatomically based on proposed in the literature. To achieve the gap of 1.5 cm, the length of the failure should exceed at least 2.5 times the diameter of the bone (BOLANDER; BALIAN, 1986;GOGOLEWSKI, 2007), in addition the periosteum and the interosseous ligament were removed to potentialize the defect.…”