Efeitos das células tronco adultas de medula óssea e do plasma rico em plaquetas na regeneração e recuperação funcional nervosa em um modelo de defeito agudo em nervo perfiférico em rato

Silva, Jefferson Braga; Gehlen, Daniel; Román, Javier; Menta, Caroline; Atkinson, Eduardo; Viezzer, Christian; Barbosa, Gustavo Leivas; Baes, Cristiane von Werne; Silva, Vinícius Duval; Costa, Jaderson Costa da

doi:10.1590/s1413-78522006000500009

Cited by 11 publications

(5 citation statements)

References 16 publications

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“…The post‐operative period used in this research was 12 weeks, suggesting that the nerve fibers haven't reached complete regenerative development, with the myelinization process still incomplete. The observation period of 12 weeks employed in this study, the same observation period of Braga‐silva et al and Gravvanis et al, appears to be insufficient for axonal maturation and growth. However, nerve fiber myelination is merely a maturity signal, allowing these fibers to conduct nerve impulses before complete myelination.…”

Section: Discussionsupporting

confidence: 52%

Effect of platelet rich plasma and platelet rich fibrin on sciatic nerve regeneration in a rat model

et al. 2013

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The aim of this study was to evaluate the effect of Platelet Rich Plasma (PRP) and Platelet Rich Fibrin (PRF) on peripheral nerve repair. Thirty-two Wistar rats were randomly divided into four equal treatments groups: autologous nerve grafts (ANG), silicon tube plus saline solution (SS), silicon tube plus PRP, and silicon tube plus PRF. In ANG group, 10 mm segment from sciatic nerve was excised and reimplanted between the nerve stumps. In the SS, PRP, and PRF groups, 5 mm segment from sciatic nerve was excised and bridged with a 12 mm silicone conduit to create a 10 mm nerve gap. The conduit was filled in accordance with the different treatments. Walking track analysis was performed periodically and on the 90th post-operative day histomorphometric analysis was performed. The ANG, PRF, and PRP groups presented a significant functional improvement in relation to the SS group (P = 0.001) on 90 days after surgery. Histomorphometric analysis demonstrated that the ANG group achieved a larger nerve fiber diameter in proximal stump while comparing with the SS group (P =0.037) and showed larger fiber diameter in median stump in comparison to the PRP group (P = 0.002) and PRF group (P = 0.001). Axonal diameter and myelin sheath thickness showed no statistical significant difference between the groups in the three stumps (P ≥ 0.05). This study suggests that PRP and PRF have positive effects on the functional nerve recovery; however, these groups don't achieve a significant improvement on the histomorphometric analysis.

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Section: Discussionsupporting

confidence: 52%

Effect of platelet rich plasma and platelet rich fibrin on sciatic nerve regeneration in a rat model

et al. 2013

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“…Other studies using stem cells removed from bone marrow in different experimental models of peripheral nerve injury observed better results in the group that used stem cells compared to the other groups to improve gait function 26 , 27 , greater number of regenerated axons or myelinated fibers 26 , 27 , 36 , faster regenerative process 26 , 36 , 38 , less muscle loss 27 , greater myelin area 36 and less Wallerian degeneration 38 . In a meta-analysis study on the use of stem cells in the regeneration of peripheral nerve defects, the regenerative effect of stem cells was demonstrated mainly by analysis of the mass of the muscles studied, electrophysiological tests and gait tests 34 .…”

Section: Discussionmentioning

confidence: 98%

Stem cells in end-to-side neurorrhaphy. Experimental study in rats

et al. 2020

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“…So that in one study, density of nerve bers stabilized at 3 months after sciatic nerve transection, but myelination was still increasing at 12 months [72]. Other studies have shown that myelination of nerve bers is not complete until 12 weeks after denervation [73]. Since the expression of S100 protein re ects the degree of myelination of nerve bers and the rate of S100 immune reactions is related to the thickness of the myelin sheath of Schwann cells [62].…”

Section: Discussionmentioning

confidence: 99%

The regenerative role of mast cells and mesenchymal stem cells in histopathology of sciatic nerve and tibialis cranialis muscle, following denervation in rats

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Shahrooz

Hobbenaghi

et al. 2022

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Atrophy of the leg muscles following sciatic nerve transection can lead to the death of myofibers. The aim of this study was to evaluate the process of nerve and muscle regeneration using mast cells and mesenchymal stem cells. In this study, 90 adult male Wistar rats weighing 150-200g were randomly divided into six main groups (n=15) and each group into three subgroups (2, 4 and 8 weeks, n=5). Groups: control; without surgery, transection (Tr); Fixing the two ends of the transected sciatic nerve (8 mm gap) in silicone tube, scaffold (S); Put collagen gel inside the silicone tube, mast cell (MC); Placed 3×104 mast cells mixed with scaffold, mesenchymal stem cell (MSC); Placed 3×104 mesenchymal stem cells mixed with scaffold, and mast cell-mesenchymal stem cell (MC+MSC); 3×104 of each of the mast cell and mesenchymal stem cells along with scaffold. Animals euthanized and sampled at weeks 2, 4 and 8 for muscle and nerve histological and nerve immunohistochemistical evaluations. Histomorphometric evaluation results of tibialis cranialis muscle (TCM) included: diameter of muscle fibers, ratio of the muscle fiber’s nuclei to the fibrocyte nuclei (mn/fn), ratio of the muscle fibers nuclei number to the muscle fiber’s number (mn/mf) and ratio of the blood vessels number to the number of muscle fibers (v/mf) in all treatment groups, especially the MC + MSC group, increased compared to the Tr group but the number of mast cells, the percentage of sarcoplasmolysis and necrosis fibers dramatically decreased. Histomorphometric evaluation results of the nerve in its various parts and immunohistochemistry results also showed improve nerve conduction in all groups, especially the MC + MSC group compared to the Tr group. All results were time dependent and in most cases at 8th week were better the other times. Overall, the results of this study showed that the simultaneous use of MCs and MSCs, especially in the eighth week, accelerated the healing of the sciatic nerve and TCM following reinnervation.

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Efeitos das células tronco adultas de medula óssea e do plasma rico em plaquetas na regeneração e recuperação funcional nervosa em um modelo de defeito agudo em nervo perfiférico em rato

Cited by 11 publications

References 16 publications

Effect of platelet rich plasma and platelet rich fibrin on sciatic nerve regeneration in a rat model

Effect of platelet rich plasma and platelet rich fibrin on sciatic nerve regeneration in a rat model

Stem cells in end-to-side neurorrhaphy. Experimental study in rats

The regenerative role of mast cells and mesenchymal stem cells in histopathology of sciatic nerve and tibialis cranialis muscle, following denervation in rats

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