Redução da oxidação na propagação in vitro da bananeira 'maçã'

Camolesi, Mara Rubia; Kaihara, Erika Sayuri; Saconi, Cibelle Garcia; Faria, Ricardo Tadeu de; Neves, Carmen Sílvia Vieira Janeiro

doi:10.1590/s1413-70542007000400044

Cited by 5 publications

(4 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Estimates of DNA content in the present study varied slightly depending on the treatment. Possible explanations for this include intrinsic factors of the vegetal matter, such as hormonal interference and a possible induction of somaclonal variation (Miguel and Marum, 2011) and external factors that involve sample preparation prior to the cytometer readings (Camolesi et al, 2007); somaclonal variation may induce morphologica, physiological and biochemical variation (Miguel and Marum, 2011;Georgiev et al, 2014) which may cause positive or negative characteristics. In morphology, the main alteration are the occurrence of variagations which is common in ornamental plants and in pineapple (Rodrigues et al, 2007) or with over growth as in Musa acuminata cv prata-anã (Albany et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Growth regulators, DNA content and anatomy in vitro-cultivated Curcuma longa seedlings

Antoniazzi¹,

Ferrari²,

Nascimento³

et al. 2016

Afr. J. Biotechnol.

View full text Add to dashboard Cite

Curcuma longa L., from the Zingiberaceae family, generally reproduces through its rhizomes, which are also utilized for therapeutic purposes because they are rich in terpenoids. Its conventional propagation has low efficiency due to the small number of seedlings and their contamination by pathogens. Therefore, this study aimed to evaluate the influence of growth regulators on the development of in vitro-cultivated C. longa as well as to determine their influence on DNA content and foliar anatomy. Shoots were inoculated in MS culture medium with the addition of 30 g/L of sucrose and 6.5 g/L of agar, and a pH adjusted to 5.8. Two assays were built to study the multiplication and rooting phases of growth. The first assay evaluated the influence of eight concentrations of cytokinins and auxins on the multiplication phase. Leaf samples were analyzed for DNA content through flow cytometry, utilizing two reference standards, green pea, and tomato. Characteristics of leaf anatomy were also measured in four time periods. The second assay analyzed the influence of six auxin concentrations on the rooting phase. The first assay showed that the root systems grew more in treatment 3 (4.44 µM benzylaminopurine [BAP], 0.46 µM kinetin [KIN]) and reached greater dry mass in T8 (8.88 µM BAP, 0.92 µM KIN, 2.16 µM naphthalene acetic acid [NAA]). The largest fresh matter of the main shoot was found in T2 (4.44 µM BAP). The estimated DNA content varied depending on the presence of supplemental growth regulators, from 2.38 to 2.77 pg, and was greater in T4 (4.44 µM BAP, 1.08 µM NAA) and T5 (4.44 µM BAP, 0.46 µM KIN, 1.08 µM NAA). Results from the latter two treatments were not significantly different. Estimates of DNA content were precise, as indicated by coefficients of variation that were much lower than 5%. The results also showed quantitative variation of evaluated anatomical traits. In general, there was a thin epidermis layer with rectangular cells, followed by parenchyma with octahedral cells and differentiated xylem and phloem. In leaf parenchyma, the presence of idioblasts containing phenolic compounds was observed in all growth stages. In the rooting phase, the supplementary auxins affected the dry matter of the aerial part and roots. The highest averages were observed in treatments with 2.0 µM NAA.

show abstract

Section: Discussionmentioning

confidence: 99%

Growth regulators, DNA content and anatomy in vitro-cultivated Curcuma longa seedlings

Antoniazzi¹,

Ferrari²,

Nascimento³

et al. 2016

Afr. J. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Para controle da oxidação, algumas propostas já foram relatadas, como a lavagem do material em água corrente antes da desinfestação para lixiviação de compostos fenólicos (Sato et al, 2001), utilização dos antioxidantes ácido ascórbico, polivinilpirrolidona (PVP), carvão ativado, cisteína (Camolesi et al, 2007;Costa et al, 2007;Sartor et al, 2013), entre outros. Os explantes de candeia, no presente trabalho, não foram lavados em água corrente, mas foram cultivados em meio contendo antioxidantes.…”

Section: Discussionunclassified

Estabelecimento in vitro de Eremanthus incanus

et al. 2019

View full text Add to dashboard Cite

Resumo -Objetivou-se avaliar procedimentos para introdução in vitro de brotações retiradas de mudas de candeia mantidas em viveiro. Para a desinfestação das brotações, testou-se tempos de imersão (5, 10, 15 e 20 min) em hipoclorito de sódio a 2,5% e a influência do uso de fungicida antes (7 e 15 dias) e após a coleta das brotações, nas concentrações de 0,5 e 1,0 g L -1 respectivamente. Para o controle da oxidação dos explantes in vitro, avaliou-se a adição de polivinilpirrolidona (PVP) e carvão ativado ao meio de cultura, ambos nas concentrações 1 e 2 g L -1 . O uso de hipoclorito de sódio permitiu a desinfestação das brotações introduzidas, porém não houve diferença significativa entre os tempos testados. A coleta de brotações 15 dias após a aplicação de fungicida nas mudas fornecedoras das brotações resultou em ausência de contaminação. A oxidação dos explantes atingiu altos níveis, mesmo na presença de PVP e carvão ativado no meio de cultura.Abstract -The objective of this study was to evaluate the procedures for introduction of shoots in vitro taken from seedlings kept in nursery. For the disinfestation of the shoots, immersion times (5, 10, 15 and 20 min) in 2.5% sodium hypochlorite were tested. We also analysed the influence of using fungicide before (7 and 15 days) and after the shoots collection, in the concentrations of 0.5 and 1.0 g L -1 respectively. In order to control the oxidation of explants in vitro, the addition of poly vinyl pyrrolidone (PVP) and activated charcoal to the culture medium, both at concentrations 1 and 2 g L -1 , were evaluated. The use of sodium hypochlorite allowed disinfestation of the introduced shoots, but there was no significant difference between the times tested. The collection of shoots 15 days after the application of fungicide in the seedlings suppliers of the shoots, allowed the absence of contamination. The oxidation of the explants reached high levels even in the presence of PVP and activated charcoal in the culture medium.

show abstract

“…Andrade, Luz e Lacerda (2000) citam que a liberação dos compostos fenólicos na cultura in vitro, é responsável pela oxidação, que comumente ocorre por meio de danos provocados às células durante a excisão dos explantes. A oxidação fenólica é causada pela reação das polifenoxidases, que por sua vez levam à produção de substâncias tóxicas, capazes de alterar a absorção de metabólitos, resultando em prejuízos significativos para o desenvolvimento dos explantes (CAMOLESI et al, 2007). No caso da babosa, a abundância dos compostos fenólicos afeta o seu crescimento in vitro, promovendo principalmente o escurecimento dos explantes (ABRIE; VAN STADEN, 2001).…”

Section: Desinfestação E Estabelecimento In Vitro Dos Explantesunclassified

Alternativa Biotecnológica para Produção de Mudas de Aloe vera L.

Ciano,

Silva,

Calixto Nery

et al. 2023

Ensaios Ciênc.

View full text Add to dashboard Cite

Espécies medicinais como a babosa (Aloe vera L.) requerem otimização do seu cultivo in vitro por meio do estabelecimento de protocolos eficazes de micropropagação, que supram carências da demanda no mercado farmacêutico e cosmético, favorecendo a diversidade biológica da babosa. Frente a isso, o objetivo deste trabalho foi ajustar uma metodologia de estabelecimento e multiplicação in vitro de plantas de babosa. As gemas foram desinfestadas em solução de hipoclorito de sódio comercial e água (1:1) e inoculadas em meio de cultura Murashige & Skoog (MS) suplementado com ácido naftalenoácético (ANA) (0,2 mg L-1) e 6-benzilaminopurina (BAP) (3,0 mg L-1).Após o estabelecimento in vitro, na fase de multiplicação, as brotações obtidas foram transferidas para meio MS, acrescido de 0,2 mg L-1 de ANA em diferentes concentrações de BAP (0,0; 1,0; 2,0; 3,0 e 4,0 mg L-1). No estudo, as gemas axilares desinfestadas em solução de hipoclorito de sódio e água, durante 20 minutos, e inoculadas em meio MS com 0,2 mg L-1 de ANA e 3,0 mg L-1 de BAP, apresentaram a maior porcentagem de explantes responsivos e isentos de contaminação em comparação com aquelas desinfestadas por 15 minutos. Por meio das características avaliadas, os maiores valores médios de altura da planta e número de brotos foram alcançados na ausência e na menor concentração testada de BAP, respectivamente. Palavras-chave: Fitocosméticos. Cultura de Tecidos. Fitoterápicos. Multiplicação In Vitro. Babosa. AbstractMedicinal species such as aloe vera (Aloe vera L.) require optimization their in vitro cultivation through establishment of effective in vitro micropropagation protocols, which supply shortages in demand in the pharmaceutical and cosmetic market, favoring the aloe biological diversity In view of this, the objective of this work was to adjust a methodology of establishment for in vitro multiplication of aloe plants. The yolks were disinfected in commercial sodium hypochlorite solution and water (1: 1) and inoculated in Murashige & Skoog (MS) culture medium supplemented with naphthaleneacetic acid (NAA) (0.2 mg L-1) and 6-benzylaminopurine (BAP) (3.0 mg L-1). After the in vitro establishment, in the stage of multiplication, the shoots obtained were transferred to MS medium, plus 0.2 mg L-1 of ANA and different concentrations of BAP (0.0, 1.0, 2.0, 3.0 and 4.0 mg L -1). In the study, axillary shoots disinfected in sodium hypochlorite and water solution (1: 1), for 20 minutes and inoculated in MS medium with 0.2 mg L-1 of ANA and 3.0 mg L-1 of BAP, presented the highest percentage of responsive and contamination-free explants, compared to those disinfected for 15 minutes . Through the evaluated characteristics, the highest average values of plant height and number of shoots were achieved in the absence and in the lowest concentration tested of BAP, respectively. Keywords: Phytocosmetics. Tissue Culture. Phytotherapics. In Vitro Multiplication. Aloe Vera.

show abstract

Redução da oxidação na propagação in vitro da bananeira 'maçã'

Cited by 5 publications

References 6 publications

Growth regulators, DNA content and anatomy in vitro-cultivated Curcuma longa seedlings

Growth regulators, DNA content and anatomy in vitro-cultivated Curcuma longa seedlings

Estabelecimento in vitro de Eremanthus incanus

Alternativa Biotecnológica para Produção de Mudas de Aloe vera L.

Contact Info

Product

Resources

About