Produção de biopolímero sintetizado por Sphingomonas capsulata a partir de meios industriais

Abstract

RESUMOCom este trabalho, avaliou-se a produção de biopolímero sintetizado por Sphingomonas capsulata ATCC 14666, utilizando-se os meios industriais melaço bruto e pré-tratado e resíduo de proteína texturizada de soja (PTS). Foram testadas diferentes concentrações de meios industriais (2,66, 4, 6 e 8%), cujas condições de fermentação utilizadas foram 28º ± 2ºC, 208 rpm, 72 h. Os ensaios foram realizados em triplicata e os resultados foram avaliados estatisticamente mediante o teste de Tukey. As melhores produtividades foram obtidas para melaço pré-tratado 8% (0,290 gL -1 h -1 ), seguida de extrato aquoso de resíduo de PTS 6% (0,240 gL -1 h -1 ) e melaço bruto 8% (0,190 gL -1 h -1 ). A qualidade reológica das gomas foi demonstrada através da leitura de viscosidade aparente de soluções aquosas e salinas a 25 e 60ºC.Termos para indexação: Biopolímeros, gênero Sphingomonas, meios industriais. ABSTRACTThis work studied the biopolymer production by Sphingomonas capsulata ATCC 14666 using industrial raw material and pretreated molasses and aqueous extract of textured soy protein (TSP). Different concentrations of industrial media (2.66, 4, 6 and 8 w/t%) were evaluated at 28º ± 2ºC, 208rpm, 72h, were the fermentation conditions utilized. Triplicate assays were conducted and Tukey s test was used. The highest productivity were obtained for 8% of pretreated molasses (0,290 gL -1 h -1 ), 6% of aqueous extract of TSP (0,240 gL -1 h -1 ) and 8% raw molasses (0,190 gL -1 h -1 ), respectively. The rheological behavior of aqueous and saline (NaCl and CaCl 2 3% wt/v) solutions of biopolymers were investigated by apparent viscosity analysis at 25 and 60ºC.

Production, extraction and characterization of exopolysaccharides produced by the native Leuconostoc pseudomesenteroides R2 strain

“…In solution, both exopolysaccharides presented a pseudoplastic behavior as the apparent viscosity decreased with an increasing shear rate. According to the literature, this behavior is expected in polymer solutions of microbial polysaccharides (Berwanger et al 2007). …”

Effect of sugarcane biopolymer gel injected in rabbit vocal fold

“…Currently there is a growing interest in the production through fermentation processes, as well as the scientific and clinical use of polysaccharides obtained byusing microorganisms capable of converting certain substances into gels, viscous solutions or membranes (biopolymers). 7 …”