Sacarose e pH na germinação in vitro de grãos de pólen de citros

Salles, Leila Aparecida; Ramos, José Darlan; Pasqual, Moacir; Junqueira, Keize Pereira; Silva, Adriano Bortolotti da

doi:10.1590/s1413-70542006000100025

Cited by 18 publications

(19 citation statements)

References 2 publications

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“…Data on the viability pollen grains and of growth of pollen germinability are key to studies about reproductive biology and breeding, as they allow greater safety to processes conducted in order to generate new hybrids and/or increase the viability and fruit production [2]. juicy red fruits.…”

Section: Introductionmentioning

confidence: 99%

“…Lectins produce three-dimensional networks of crosslinked glycoconjugates, which allow their detection, due to the cellular precipitation or clumping [4]. According Salles et al [2], in a study with in vitro pollen germination of Citrus, pollen tube growth is stopped before it gets the size usually reached in the stigma, because of the medium composition and pH-factors that affect the formation of the pollen tube.…”

Section: Introductionmentioning

confidence: 99%

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Viability and Action of CPL Lectin on in Vitro Germinability of Pollen Grains of Malpighia emarginata DC.—(Malpighiaceae)

Sousa¹,

Rego²,

Santos³

2013

AJPS

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This study aimed to observe the viability of the pollen grains of Malpighia emarginata DC. (West Indian cherry) and the action of a lectin in their germination. Lectins are proteins that specifically interact with carbohydrates, but don't modify them and bind with high affinity and specificity, promoting a transfer of information that is clearly central to many cellular processes in living beings. For the viability test was used aniline blue in lactophenol. The in vitro germination test used was the hanging drop method, in control medium containing sucrose, boric acid, calcium nitrate and agar. The experiment dealt with three different treatments in order to pollen germination: growing medium without addition of lectin, with 1.0 µg/ml and with 3.0 µg/ml CPL lectin. This lectin is extracted from seeds of Crotalaria pallida L.-Leguminosae. Data on pollen grains were analyzed by one-way ANOVA, with 95% reliability and comparison of averages by Turkey test at 5% probability. It was found that despite previous high viability of the pollen grains (c. 70%), a germinability rate has been low, yet exceptionally larger in pollen samples placed on a growing medium with addition of 3.0 µg/ml of CPL lectin (24%). There was no significant difference among the pollen grains germinate in medium without lectin and those in medium with the addition of only 1.0 µg/ml. According to data obtained in the treatments, CPL lectin, with concentration of 3.0 µg/ml, influenced the formation of the pollen tube and thus more pollen germinated in Malpighia emarginata.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Viability and Action of CPL Lectin on in Vitro Germinability of Pollen Grains of Malpighia emarginata DC.—(Malpighiaceae)

Sousa¹,

Rego²,

Santos³

2013

AJPS

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“…En programas de mejoramiento, el conocimiento de la viabilidad del polen es uno de los factores esenciales, sobre todo cuando se utilizan técnicas de hibridación artifi cial, como es el caso del tomate de cáscara. La determinación de la viabilidad del polen y su importancia en estudios de la biología de la reproducción y mejoramiento genético de plantas, es fundamental para definir la dirección de una cruza y contar con bases para el éxito de hibridaciones controladas para garantizar la formación de nuevos híbridos e incrementar su viabilidad (Salles et al, 2006). De ahí la necesidad de estimar la viabilidad el polen en autotetraploides de tomate de cáscara.…”

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Viabilidad de polen, densidad y tamaño de estomas en autotetraploides y diploidesde Physalis ixocarpa

Ramírez-Godina¹,

Robledo-Torres²,

Foroughbakhch³

2013

Bot. Sci.

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4Autor para la correspondencia: varoto@prodigy.net.mx Resumen: Con el objetivo de estudiar el impacto de la autopoliploidía en la viabilidad de polen y el tamaño y frecuencia estomá-tica del tomate de cáscara (Physalis ixocarpa) se analizó el polen, tamaño y número de estomas de cinco poblaciones diploides y cinco autotetraploides formadas con colchicina. Las diez poblaciones se establecieron en campo, bajo un diseño de bloques al azar con cuatro repeticiones. Para el estudio de viabilidad de polen, éste fue colectado y coloreado con acetocarmín al 1%. Los granos de polen redondeados y coloreados de rojo se consideraron viables y los constreñidos y sin teñir, no viables. Además, se estimó la densidad e índice estomático, largo y ancho de células oclusivas de estomas de la superfi cie adaxial y abaxial de la hoja. Los diploides tuvieron valores de viabilidad de polen y densidad estomática, signifi cativamente mayores que los autotetraploides (P ≤ 0.05); sin embargo, los diploides tuvieron granos de polen y estomas signifi cativamente más pequeños que los autotetraploides. Por lo tanto, la poliploidía si afectó la viabilidad del polen, ocasionado pérdida de fertilidad. Sin embargo, esta característica se puede evitar por selección de plantas con estabilidad meiótica, para incrementar la fertilidad de los autotetraploides. El aumento del tamaño celular muestra que los autopoliploides tienen el potencial de generar plantas más vigorosas, pero con una disminución en la fertilidad, aunque esta característica puede mejorarse al paso de varias generaciones, por lo que este tipo de estudios abre la posibilidad de iniciar nuevas estrategias para el mejoramiento del tomate de cáscara. Palabras clave: abaxial, adaxial, granos de polen, poliploidía, tomate de cáscara.

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“…According to Salles et al (2006), pollen tubes may break due to high humidity and changes in the environment caused by increased osmotic pressure and low resistance of the cell wall. Boron stimulates pollen tube growth and decreases the likelihood of breaking up (FRANZON; RASEIRA, 2006).…”

Section: Resultsmentioning

confidence: 99%

In vitrogermination and viability of pollen grain of coconut accessions

Moura¹,

Machado²,

Lédo³

2015

Revista Ciência Agronômica

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-Storage as a means of maintaining the pollen viability is important for the preservation of the genetic variability, facilitates the exchange of germplasm and greatly contributes to the generation of variability obtained from artificial crosses, increasing the efficiency of breeding programs. The objective of this study was to select different culture media for the in vitro germination of pollen grain of dwarf and tall coconut accessions, as well as to determine the viability of pollen grain at room temperature conditions. For this purpose, Brazil Green Dwarf (BGD) and Brazilian Tall (BRA) pollen grains derived from the Coconut Active Germplasm Bank of Embrapa Coastal Tablelands Sergipe were used. To evaluate the effect of different culture media on the in vitro germination of pollen grains of anão verde do Brasil de Jiqui (AVeBrJ) and gigante do Brasil Praia do Forte (GBrPF) accessions, they were inoculated on to Petri dishes containing 2 ml of culture media. The pollen viability was assessed by staining with 1% acetic carmine and in vitro germination at 0, 24, 48 and 72 hours. The culture medium of Lora is suitable to assess the in vitro germination of pollen grain of the AVeBrJ and GBrPF accessions. The pollen grain of the AVeBrJ accession showed intermediate viability (66.87%) at room temperature up to 23.14 hours by in vitro germination. The pollen grain of the GBrPF accession showed high viability, above 70%, at room temperature up to 120 hours by in vitro germination.

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Sacarose e pH na germinação in vitro de grãos de pólen de citros

Cited by 18 publications

References 2 publications

Viability and Action of CPL Lectin on <i>in Vitro</i> Germinability of Pollen Grains of <i>Malpighia emarginata</i> DC.—(Malpighiaceae)

Viability and Action of CPL Lectin on <i>in Vitro</i> Germinability of Pollen Grains of <i>Malpighia emarginata</i> DC.—(Malpighiaceae)

Viabilidad de polen, densidad y tamaño de estomas en autotetraploides y diploidesde Physalis ixocarpa

In vitrogermination and viability of pollen grain of coconut accessions

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Sacarose e pH na germinação in vitro de grãos de pólen de citros

Cited by 18 publications

References 2 publications

Viability and Action of CPL Lectin on &lt;i&gt;in Vitro&lt;/i&gt; Germinability of Pollen Grains of &lt;i&gt;Malpighia emarginata&lt;/i&gt; DC.—(Malpighiaceae)

Viability and Action of CPL Lectin on &lt;i&gt;in Vitro&lt;/i&gt; Germinability of Pollen Grains of &lt;i&gt;Malpighia emarginata&lt;/i&gt; DC.—(Malpighiaceae)

Viabilidad de polen, densidad y tamaño de estomas en autotetraploides y diploidesde Physalis ixocarpa

In vitrogermination and viability of pollen grain of coconut accessions

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Viability and Action of CPL Lectin on <i>in Vitro</i> Germinability of Pollen Grains of <i>Malpighia emarginata</i> DC.—(Malpighiaceae)

Viability and Action of CPL Lectin on <i>in Vitro</i> Germinability of Pollen Grains of <i>Malpighia emarginata</i> DC.—(Malpighiaceae)