Receptiveness of the stigma and in vitro germination of orange pollen, submitted to different temperatures

Pio, Leila Aparecida Salles; Ramos, José Darlan; Pasqual, Moacir; Santos, Flávia Carvalho; Junqueira, Keize Pereira

doi:10.1590/s1413-70542004000500016

Cited by 3 publications

(4 citation statements)

References 5 publications

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“…In tests based on staining the stigma with hydrogen peroxide (peroxide water), it is assumed that the rate of bubble production from the stigmatic surface in hydrogen peroxide is directly related to the level of stigma receptivity [ 10 ]. Therefore, if the peroxidase enzyme is present, many oxygen bubbles will be released by the chemical reaction of the peroxide with the enzyme [ 11 ]. A preliminary design was used to evaluate the level of oxygen generation.…”

Section: Methodsmentioning

confidence: 99%

Intraspecific Crossability inAndrographis paniculataNees: A Barrier against Breeding of the Species

Valdiani

Kadir

Saad³

et al. 2012

The Scientific World Journal

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The ambiguity of crossability in Andrographis paniculata (AP) was pointed out in the present research. Accordingly, the effects of different style length and crossing time on intraspecific crossability of seven AP accessions in 21 possible combinations were investigated. The best results came out between 08:00 to 11:00 h for manual out-crossing of AP, while the time from 12:00 to 18:00 h showed a decreasing trend. Moreover, 12 mm style length was found as the most proper phenological stage in terms of stigmatic receptivity to perform out-crossing in this plant. All in all, AP behaved unlikely in each combination, and a significant difference was observed in crossability of AP accessions (P < 0.01). The lowest and highest crossability rate was found in hybrids 21 (11261NS × 11344K) and 27 (11322PA × 11350T) with 0.25% and 13.33%, respectively. Furthermore, a significant negative relationship between style length and crossibility (r 2 = 0.762∗∗) was recorded in this research. As a final conclusion, crossing time and proper style length can improve the intraspecific crossability in the species, considerably. Despite all the mentioned contrivances, we still believe that a genetic incongruity should be involved as an additional obstacle in crossability of those combinations that failed or responded deficiently to outcrossing.

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Section: Methodsmentioning

confidence: 99%

Intraspecific Crossability inAndrographis paniculataNees: A Barrier against Breeding of the Species

Valdiani

Kadir

Saad³

et al. 2012

The Scientific World Journal

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“…PIO et al (2004) afirmaram que, além da composição do meio, a temperatura e a alta umidade podem ocasionar aumento na pressão osmótica e baixa resistência na parede celular, causando assim o rompimento ou eclosão dos grãos de pólen, impedindoos de germinar.…”

Section: Resultsunclassified

“…Para Citrus, a temperatura ideal para a germinação dos grãos de pólen foi de 25ºC, similar a de muitas espécies citadas na literatura, em que os melhores índices de germinação ocorrem no intervalo de 20ºC a 30ºC (PIO et al, 2004). A constatação de uma temperatura ideal na germinação in vitro pode ter uma implicação prática importante, Tabela 1 -Medidas de grãos de pólen acetolisados e espessura da exina através da microscopia eletrônica de transmissão de acessos de abacaxizeiros ornamentais.…”

Section: Resultsunclassified

Morfologia e viabilidade de grãos de pólen de acessos silvestres de abacaxi

et al. 2011

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O objetivo do trabalho foi avaliar a viabilidade de grãos de pólen de acessos silvestres de abacaxi mediante a percentagem de germinação in vitro, crescimento do tubo polínico em duas temperaturas de incubação e uso de carmim acético. Além disso, o trabalho busca reconhecer os padrões da morfologia polínica de algumas variedades de abacaxi. Foram utilizadas amostras de pólen coletadas de seis acessos de abacaxi: Silvestre-25, I-26/803 (Ananas macrodontes), Igor (A. comosus var. erectifolius), Ananás Santo Amaro, FRF-22 e FRF-32 (A. comosus var. bracteatus). Os grãos de pólen foram inoculados em meio de cultura contendo 10% de sacarose, 0,01% H3BO3, 0,01% KNO3, 0,03% Ca(NO3)2.4H2O, solidificado com 1% de ágar e pH ajustado para 6,5. Foram testadas as temperaturas de incubação de 25°C e 30°C. A viabilidade do pólen foi avaliada pela coloração com carmim acético a 2%. Todas as variedades de A. comosus apresentaram grãos de pólen de tamanho médio, simetria radial, âmbito ovalado a esferoidal, 2-porados, subprolatos, exina eutectada, reticulada, heterobrocada, lumens variando de arredondados a poligonais, sem granulações, com exceção dos dois acessos de A. macrodontes que apresentaram tamanho grande. Melhores taxas de germinação foram obtidas para A. macrodontes em comparação com o erectifolius e bracteatus. A maioria dos acessos apresentou viabilidade com carmim acético acima de 76%.

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“…It is noteworthy that there are several factors that might affect the pollen germination and pollen tube growth in the examined H. conoidea pollen. One of which is the temperature, the very basic factor controlling the environmental conditions and influences pollen germination and longevity in stored pollen [63,64,65]. The pollen germination rate is also greatly influenced by the pH of germination medium, which has also been shown in several plant species [39, 66 -71] and relative humidity and composition of the germination medium [72,73].…”

Section: Pollen Germination and Pollen Tube Growthmentioning

confidence: 99%

In vitro Studies on Pollen Viability, Pollen Germination and Pollen Tube Growth of Hornstedtia conoidea Ridl. – a Philippine Endemic Ginger Species

Mendez

Acma²

2018

J.Trop.Life.Science

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Zingiberaceous plants are predominantly propagated through underground rhizomes and seeds. In this study, the viability of pollen, rate of pollen germination and length of pollen tube of the Philippine endemic Hornstedtia conoidea were examined. Four petri plates containing pollen samples were prepared, of which two petri plates were used immediately after the collection, while the other two petri plates were stored for one week. The determination of pollen viability was made employing stain tests using IKI (iodine + potassium iodide) solution and safranin and measured immediately after the collection and after one week. Likewise, pollen germination test was carried out in vitro on agar medium and measured after 24 hours and one week of incubation. Data revealed that pollen viability was higher in the samples which were sown immediately with safranin with percentage pollen viability of 92.43%, while IKI test was lower with 89.36%. On the other hand, the pollen stored for one week measured 47.29% for safranin test and 33.14% for IKI test. The percentage germination of pollen after 24 hours was 72.65%, while 11.13% after one week. The pollen samples which were subjected for tube growth were examined for two weeks and gave positive results. Overall, it can be concluded that H. conoidea pollen can successfully be collected and stored for certain time. Knowledge regarding the pollen viability, pollen germination and pollen tube growth of this species will give practical benefit for plant breeding and conservation purposes, since H. conoidea is found in the wild, and as of now, the species has been recorded only in the Philippines.

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Receptiveness of the stigma and in vitro germination of orange pollen, submitted to different temperatures

Cited by 3 publications

References 5 publications

Intraspecific Crossability inAndrographis paniculataNees: A Barrier against Breeding of the Species

Intraspecific Crossability inAndrographis paniculataNees: A Barrier against Breeding of the Species

Morfologia e viabilidade de grãos de pólen de acessos silvestres de abacaxi

In vitro Studies on Pollen Viability, Pollen Germination and Pollen Tube Growth of Hornstedtia conoidea Ridl. – a Philippine Endemic Ginger Species

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