Variação da composição química do meio de cultura e da bioquímica celular de Phaeodactylum tricomutum (Bohlin), em cultivos estanques

Aidar, Elizabeth; Ehrlich, Roberto; Asano, Cristina Sayuri; Sigaud, Teresa Cristina Siqueira

doi:10.1590/s0373-55241991000200004

Cited by 3 publications

(2 citation statements)

References 15 publications

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“…In accordance with Aidar et al (1991), the highest phosphate absorption rates occurred during the lag phase of cell growth, indicating that phosphate absorption occurred immediately. However, signifi cant increase in biomass only occurred late in the exponential phase.…”

Section: Domênica Teixeira De Lima Fernanda Reinhardt Piedras Flávisupporting

confidence: 70%

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Intraspecific variation in alkaline phosphatase activity in Phaeodactylum tricornutum (Bacillariophyceae, Bohlin)

et al. 2016

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To describe potential intraspecifi c variation in phosphorus incorporation in two strains of Phaeodactylum tricornutum (Bohlin), Ub3 and Ub7, alkaline phosphatase (AP) activity was evaluated via enzyme-labeled fl uorescence assay. Analysis using the probe ELF-97 ® provides individual evaluation, and therefore can determine the nutritional status of inorganic phosphorus in phytoplanktonic cells. Bioassays compared the control treatment to both phosphateenriched and phosphate-depleted treatments by varying only the phosphate concentration in the media. Th e P. tricornutum strains exhibited diff erences in their development when incubated in the phosphate-enriched media. Th e development of the Ub7 strain diff ered by exhibiting "luxury uptake" and utilization of organic phosphorus, and the alkaline phosphatase analysis indicated limitations of this clone under such conditions. Th e Ub7 strain showed higher AP activity, when compared to Ub3, in the P-enriched condition. P. tricornutum presented increases in AP activity and low variation in Surface/Volume ratio, by increasing biovolume and its maximum linear dimension, as strategies for phosphate incorporation. Our results highlight intraspecifi c diff erences in alkaline phosphatase activity, and hence diff erences in the incorporation of organic phosphorus, as the tested species regulated enzymatic activity under diff erent external phosphate concentrations.

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Section: Domênica Teixeira De Lima Fernanda Reinhardt Piedras Flávisupporting

confidence: 70%

“…As with growth rate, maximum cell density can vary considerably among strains of the same species under the same culture conditions (i.e. Aidar et al 1991;Voltolina et al 1998;Foster et al 2008;Ohse et al 2008;Lin et al 2013).…”

Section: Resultsmentioning

confidence: 99%

Intraspecific variation in alkaline phosphatase activity in Phaeodactylum tricornutum (Bacillariophyceae, Bohlin)

et al. 2016

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Changes in biochemical profile of Tetraselmis gracilis I. Comparison of two culture media

et al. 1997

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A microcosm approach on the potential effects of the vertical mixing of water masses over the primary productivity and phytoplankton biomass in the southern Brazilian coastal region

Saldanha-Corrêa

Gianesella

2004

Braz. j. oceanogr.

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The vertical mixing between South Atlantic Central Water (SACW) and Coastal Water (CW) was simulated through microcosm experiments using the autochthonous phytoplankton community (fraction < 150 mm), without nutrient enrichments. SACW is cold (T< 18°C) and nutrient rich, while CW is warmer (T> 20°C) and oligotrophic. The phytoplankton growth potential of SACW, CW and an equivalent mixture of both (SACW+CW) was compared, under 100, 30 and 10% of sunlight, at surface seawater temperature, in winter and summer conditions. Results demonstrate the importance of SACW as a natural eutrophication agent for the mixing layer, allowing the occurrence of new production by nutrient input, and also as a biological seeder through the development of its autochthonous phytoplankton community when it reaches the euphotic zone. The time lag for phytoplankton development during winter was around 4-5 days, against 1-2 days in summer. The hypothesis of physiological differences between surface and bottom phytoplankton populations from a deep (80 m) and thermally homogeneous water column (common winter feature) was also tested through the microcosm experiments. Results obtained clearly demonstrate that bottom water presented higher phytoplankton growth potential than the surface one.
A mistura vertical entre a Água Central do Atlântico Sul (ACAS) e a Água Costeira (AC) foi simulada através de experimentos tipo microcosmos, com o fitoplâncton autóctone (fração < 150 mm) e sem enriquecimentos nutricionais. A ACAS é fria (T< 18°C) e rica em nutrientes, enquanto a AC é mais quente (T> 20°C) e oligotrófica. O potencial trófico dessas águas e de uma mistura equivalente de ambas (ACAS+AC) foi comparado a 100, 30 e 10% da luz solar, sob temperatura da água do mar na superfície, em condições de inverno e verão. Os resultados demonstram a importância da ACAS como agente fertilizador da camada de mistura tanto por introduzir nutrientes, favorecendo a ocorrência de produção nova, como pelo incremento da biomassa fitoplanctônica autóctone ao atingir a zona eufótica. A fase de adaptação observada no inverno foi de 4-5 dias enquanto no verão foi de 1-2 dias. A hipótese da existência de diferenças fisiológicas entre as populações fitoplanctônicas de superfície e fundo em uma coluna de água profunda (80 m) e termicamente homogênea (padrão comum de inverno) foi também testada utilizando microcosmos. Os resultados mostraram que potencial de crescimento fitoplanctônico nas águas de fundo foi significativamente maior que nas de superfície

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Variação da composição química do meio de cultura e da bioquímica celular de Phaeodactylum tricomutum (Bohlin), em cultivos estanques

Cited by 3 publications

References 15 publications

Intraspecific variation in alkaline phosphatase activity in Phaeodactylum tricornutum (Bacillariophyceae, Bohlin)

Intraspecific variation in alkaline phosphatase activity in Phaeodactylum tricornutum (Bacillariophyceae, Bohlin)

Changes in biochemical profile of Tetraselmis gracilis I. Comparison of two culture media

A microcosm approach on the potential effects of the vertical mixing of water masses over the primary productivity and phytoplankton biomass in the southern Brazilian coastal region

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