In vitro assessment of the bactericidal effect of low-power arsenium-gallium (AsGa) laser treatment

Costa, Adilvania Ferreira da; Assis, Juvêncio César Lima de

doi:10.1590/s0365-05962012000400029

Cited by 8 publications

(4 citation statements)

References 4 publications

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“…Costa (23) using the low power laser, AsGa, 904 nm, at a dosage 6 J/cm2 in Pseudomonas aeruginosa and Staphylococcus aureus observed no growth inhibition halo on Braoios A, Prado RP MTP&RehabJournal 2015, 13: 304 the plates and concluded that laser therapy (AsGa, 904 nm, 6 J/cm²) produced no bactericidal effect.…”

Section: Discussionmentioning

confidence: 99%

Analysis of low level laser therapy in vitro cultures of bacteria and fungi

Andraus¹,

Maia²,

Santos³

et al. 2015

mtprehabjournal

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Background: Low intensity laser therapy has been used to accelerate the ulcers healing due to its healing, anti-inflammatory, analgesic and anti-edematous effects. Contaminations by bacteria or fungi on these ulcers commonly happen, constituting a major complication of healing, but there is no consensus about laser therapy bactericidal or fungicidal effect. Aim: To analyze the effects of different times of appliantion using the 808nm and 660nm lasers on cultures of different bacterial species and fungi in vitro. Method: Photon Lase III -DMC was used, with the wavelengths of 660 and 808nm and 30mW, 60mW and 100mW of power, with different exposure time to laser irradiation. Fifteen Petri plates were prepared, 12 plates containing Gram-positive (3 Staphylococcus aureus plates, 3 nterococcus plates) and gram negative bacterias (3 Escherichia coli, 3 Klebsiella pneumonia plates) and 3 Petri plates with the fungi Candida albicans. Plates were randomly divided into 3 groups with 5 plates each group. Plates of Group 1 were irradiated with 660 nm laser at nine different points (A, A 1 , A 2 /B, B 1 , B 2 /C, C 1 , C 2 ), and points A/B/C reciebed only one application, points A 1 ,B 1 ,C 1 received 2 applications and points A 2 ,B 2 ,C 2 received 3 applications. Plates from group 2 where irradiated with 660nm also in nine diferente points, following the same criterea of group 1, while plate of group 3 where used as control, without irradiation. Groups 1 and 2 were irradiated with 144 J/cm² dose at point A (irradiation time of 2 minutes and 15 seconds), point B (irradiation time of 1 minute 7 seconds) and at point C (40 seconds). After the irradiations the plates were incubated for 24 hours. The cultures were visually examined to check the presence or not of the inhibition zone. In all the groups, no zone of inhibition nor growth were observed. Conclusion: Low intensity laser therapy (808nm and 660nm) did not produce bactericidal and/or bacteriostatic effects, and even no bio-stimulant effect on the bacteria.

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Section: Discussionmentioning

confidence: 99%

Analysis of low level laser therapy in vitro cultures of bacteria and fungi

Andraus¹,

Maia²,

Santos³

et al. 2015

mtprehabjournal

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“…In vitro studies suggest that light at wavelengths between 625-1000 nm (from red to infrared) when applied alone does not induce increased growth of bacteria such as S. aureus and P. aeruginosa (Lipovsky et al, 2010;Lee et al, 2011;Baffoni et al, 2012;Costa and Assis, 2012;Araujo et al, 2013;Kim et al, 2013;Pereira et al, 2014). This suggests that such wavelengths do not interact with chromophores known to exist in these infectious agents.…”

Section: Discussionmentioning

confidence: 99%

Blue and red light photoemitters as approach to inhibit Staphylococcus aureus and Pseudomonas aeruginosa growth

Galo

Prado²,

Santos

2022

Braz. J. Biol.

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The ability of pathogenic bacteria acquire resistance to the existing antibiotics has long been considered a dangerous health risk threat. Currently, the use of visible light has been considered a new approach to treat bacterial infections as an alternative to antibiotics. Herein, we investigated the antimicrobial effect of two range of visible light, blue and red, on Staphylococcus aureus and Pseudomonas aeruginosa, two pathogenic bacterial commonly found in healthcare settings-acquired infections and responsible for high rate of morbidity and mortality. Bacterial cultures were exposed to blue or red light (470 nm and 660 nm) provided by light-emitting diodes - LED. The fluencies and irradiance used for blue and red light were 284.90 J/cm2, 13.19 mW/cm2 and 603.44 J/cm2, 27.93 mW/cm2 respectively. Different experimental approaches were used to determine the optimal conditions of light application. Only exposure to blue light for 6 hours was able to inhibit about 75% in vitro growth of both bacterial species after 24 hours. The surviving exposed bacteria formed colonies significantly smaller than controls, however, these bacteria were able to resume growth after 48 hours. Blue light was able to inhibit bacterial growth upon inoculation in both saline solution and BHI culture medium. We can conclude that blue light, but not red light, is capable of temporarily retarding the growth of gram negative and gram positive bacteria.

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“…Os grupos submetidos à Laserterapia-V nas doses 150, 225 e 300J/cm² e à Laserterapia-I na dose 225J/cm² não inibiram o crescimento das bactérias testadas neste estudo. Esses resultados corroboram os encontrados por Costa e Assis 26 (2012), evidenciando não haver qualquer efeito bactericida ou bacteriostático do laser utilizado com os parâmetros em questão. Esses pesquisadores verificaram o efeito bactericida in vitro do laser de baixa potência, AsGa, 904 nm, na dose 6J/cm 2 .…”

Section: Discussionunclassified

Efeito in vitro da laserterapia e da terapia fotodinâmica na redução de bactérias presentes em canais radiculares

Grando

Bianchini

Grazziotin

et al. 2021

Rev. da Fac. de Odontologia, UPF

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Este estudo tem por objetivo verificar in vitro o efeito bactericida da laserterapia e da terapia fotodinâmica com laser de baixa potência (660 nm e 808 nm) em bactérias presentes nos canais radiculares. Métodos: foram preparadas 60 placas de Petri com bactérias: 20 placas com Enterococcus faecalis, 20 placas com Staphylococcus aureus e 20 com Pseudomonas aeruginosa. Aleatoriamente, dividiu-se cada grupo em 10 subgrupos (duas placas cada): três subgrupos tratados com laserterapia 660 nm em doses de 150, 225 e 300J/ cm², três subgrupos tratados com terapia fotodinâmica (azul de metileno 0,2% e laser 660 nm) em doses de 150, 225 e 300J/cm²; um subgrupo tratado com laserterapia 808 nm na dose de 225J/cm², um subgrupo com terapia fotodinâmica e laser 808 nm, em dose 225J/cm²; um subgrupo tratado apenas com fotossensibilizante (FS), e um não tratado (controle). Os tratados com laserterapia e terapia fotodinâmica foram irradiados uma única vez e incubados por 24 horas. Os últimos dois não receberam irradiação. As culturas foram analisadas visualmente para verificação do halo de inibição. Nos grupos submetidos somente à laserterapia, para o grupo FS e para o grupo controle, não foram observados halos de inibição, já onde houve aplicação da TFD, tanto com L1 quanto com L2, observaram-se halos de inibição em todas as espécies bacterianas estudadas. Conclui-se que a laserterapia, não produziu efeitos bactericidas e/ou bacteriostáticos, enquanto a terapia fotodinâmica nos dois comprimentos de onda produziu halos significativos de inibição de crescimento nas três bactérias do estudo.

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In vitro assessment of the bactericidal effect of low-power arsenium-gallium (AsGa) laser treatment

Cited by 8 publications

References 4 publications

Analysis of low level laser therapy in vitro cultures of bacteria and fungi

Analysis of low level laser therapy in vitro cultures of bacteria and fungi

Blue and red light photoemitters as approach to inhibit Staphylococcus aureus and Pseudomonas aeruginosa growth

Efeito in vitro da laserterapia e da terapia fotodinâmica na redução de bactérias presentes em canais radiculares

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