Seleção de matéria prima para a elaboração de meio de cultura para produção de Bacillus thuringiensis var. kurstaki Berliner

Abstract

Selection of Agroindustrial By-Products as Components of Media Used for Production of Bacillus thuringiensis var. kurstaki Berliner ABSTRACT-The spore production "in vitro" of 43 combinations of agroindustrial by-products (cheese whey, silkworm flour, soybean protein and foder yeast) was evaluated. All media tested showed to be suitable for Bacillus thuringiensis var. kurstaki Berliner production and the concentration of spores obtained in these media varied from 10 6 to 10 9 spores/ml.

Production of Bacillus thuringiensis Berliner var. kurstaki Grown in Alternative Media

“…For the initial medium selection, 43 combinations of ingredients were tested including: fodder yeast, soya bean milk and protein from leguminous seeds (Phaseolus vulgaris and Glycine max ), cheese whey, sugar cane molasses, ground Bombyx mo ri pupae, glycerol, sucrose, industrial residue of the production of monosodium glutamate, and salts (CaC0 3 and MnS0 4 , H 2 0). Methods and results are described in Alves et al (1997).…”

“…Based on results described in Alves et al (1997), three media (Table 1) were selected for further testing. These three media were selected for their high spore yields and availability of ingredients in Brazil (Alves et a!., 1997). Nutrient broth + 0.1% yeast extract (NBY; Difco Labs Ltd) was used as the standard for comparison.…”

Produção De Conídios De Metarhizium Anisopliae Em Meio Sólido À Base De Resíduos Agroindustriais

“…Segundo ottati- de-liMa (2007), devido ao elevado preço que o arroz vem alcançando no comércio, o custeio do substrato para o fungo tem acarretado despesas crescentes aos fabricantes. Estudos têm sido realizados desde a década de 80 com o objetivo de avaliar substratos alternativos e mais baratos, incluindo resíduos agroindustriais (Cruz et al, 1983;alvarenga et al, 1988;Magalhães;Frazão, 1996;vilas Boas et al, 1996;alves et al, 1997a;alves et al, 1997b;dalla santa et al, 2005), havendo uma tendência para a regionalização da produção (alves et al, 1997b). Tal tendência se intensificou nos últimos anos com o apoio do Instituto Biológico, o qual vem prestando consultoria na instalação de biofábricas do fungo M. anisopliae para o controle biológico da cigarrinha da cana- de-açúcar (leite et al, 2003).…”