A potential economical substrate for large-scale production of<i>Bacillus thuringiensis</i>var.<i>kurstaki</i>for caterpillar control

Paul, Bishwajeet; Paul, Sangeeta; Khan, Mohammad Zafar

doi:10.1080/09583157.2011.605942

Cited by 4 publications

(3 citation statements)

References 12 publications

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“…israelensis , widely used in mosquito control programs. Paul et al ( 2011 ) develop an economical medium, based on inexpensive, locally available raw materials. They reported that Parthenium hysterophorus L. leaf extract-based culture medium resulted in highest toxicity (LC 50 14.628 μg/ml) against 7-day-old Spodoptera litura (Fab) larvae.…”

Section: Cost-effective Medium For Large-scale Productionmentioning

confidence: 99%

Potential of Bacillus thuringiensis in the Management of Pernicious Lepidopteran Pests

Khan

Paul

Ahmad

et al. 2016

Plant, Soil and Microbes

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Microbial products have a long history of safe use and most of the microbial agents are compatible with other methods of pest control. A number of microbial biopesticides have been registered for fi eld application on various vegetables, fruits, and other crops of agricultural, horticultural, and forest importance. During sporulation phase, Bacillus thuringiensis accumulates certain insecticidal crystal proteins which are pathogenic to a number of insect orders. Thousands of toxicogenic strains of B. thuringiensis exist and each strain produces its own unique well-known insecticidal crystal protein. B. thuringiensis is biodegradable and safe to nontarget organisms as the conditions required for complex steps in the mode of action do not exist in mammals or most of invertebrates. Development of agricultural crop varieties that contain B. thuringiensis proteins provides a safe alternative to the use of chemical insecticides. Tobacco and tomato were the fi rst transgenic plants encoding for B. thuringiensis insecticidal crystal protein. The development of resistance to B. thuringiensis toxins is, however, particularly unfortunate. Thousands of B. thuringiensis isolates are available around the world, and fortunately almost all the major insect pests are susceptible to these strains. Moreover synthetic insecticides in combination with biopesticides are economic, 278 effective, and eco-friendly. The aim of this chapter is to focus on the potentiality of B. thuringiensis in the management of pernicious lepidopteran pests and their mode of their interactions to develop the cost-effective medium for the formulation of biopesticides.

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Section: Cost-effective Medium For Large-scale Productionmentioning

confidence: 99%

Potential of Bacillus thuringiensis in the Management of Pernicious Lepidopteran Pests

Khan

Paul

Ahmad

et al. 2016

Plant, Soil and Microbes

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“…Furthermore, it was stated that the concentration of nutritional components in the growth media of B. thuringiensis, such as glucose and mineral salts, could increase the spore production (Amin et al, 2008;Sarrafzadeh, 2012). The development of B. thuringiensis cells was better when the nutritional content of the growth media was more complete, including vitamins and minerals (Paul et al, 2011).…”

Section: Mortality=mentioning

confidence: 99%

The Use of Golden Snail Meal to Enrich Bacillus Thuringiensis Culture Media and Its Effect on the Bacterial Toxicity Against Spodoptera Litura

Pujiastuti

Masyitah²,

Dirgahayu³

et al. 2018

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

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The use of golden snail meal to enrich Bacillus thuringiensis culture media and its effect on the bacterial toxicity against spodoptera litura. Bacillus thuringiensis is an entomopathogenic bacterium producing spore and protein at sporulation. There has been limited research on using golden snail as protein resource to enrich bacterial culture media. This research was aimed at studying bacterial cell/spore production in culture media made from coconut water media and liquid waste of tofu industry (tofu whey) enriched with golden snail meal (GSM), as well as its toxicity against Spodoptera litura. The research was conducted in the Laboratory of Entomology, Department of Plant Protection, Faculty of Agriculture, Sriwijaya University, from June to September 2017. The experiment was arranged in a Factorial Completely Randomized Design (FCRD) with two factors and three replications. The first factor was the addition of GSM (0, 5, 9, 13 and 17 g) and the second was storage longevity (0, 1, and 2 months). Number of test insect (third instar of S. litura) was 30 larvae per treatment. Parameter observed were spore density, larval mortality, and symptom of infected larvae. The results showed that B. thuringiensis cultured in culture media enriched with 13 g golden snail meal produced the highest spore density, amounted to 14.14 x 107spores/ml and caused larval mortality up to 86.67%. After one and two month storage treatments, the spore density in the cultures reduced to 2.51 x 107 and 1.49 x 107 spores/ml, respectively. There was a tendency of reduction in spore density under longer storage of the culture. Survived larvae developed abnormally, failed to transform to pupa or imago.

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“…thuringiensis production. Agroindustrial residues and byproducts like cheese whey, soya bean milk, molasses, chicken feather waste, and paddy husk waste have been used as ingredients [ 6 – 8 ]. Edible seeds like mung beans ( Vigna radiata (L.) R. Wilczek) were used as major sources of protein together with different combinations of soluble starch and/or sugarcane molasses as major carbohydrate sources for the production of delta-endotoxin [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Different Culture Media for Improvement in Bioinsecticides Production by IndigenousBacillus thuringiensisand Their Application against Larvae ofAedes aegypti

Devidas

Pandit

Vitthalrao

2014

The Scientific World Journal

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Production of indigenous isolate Bacillus thuringiensis sv2 (Bt sv2) was checked on conventional and nonconventional carbon and nitrogen sources in shake flasks. The effects on the production of biomass, toxin production, and spore formation capability of mosquito toxic strain were determined. Toxicity differs within the same strain depending on the growth medium. Bt sv2 produced with pigeon pea and soya bean flour were found highly effective with LC50 < 4 ppm against larvae of Aedes aegypti. These results were comparable with bacteria produced from Luria broth as a reference medium. Cost-effective analyses have revealed that production of biopesticide from test media is highly economical. The cost of production of Bt sv2 with soya bean flour was significantly reduced by 23-fold. The use of nonconventional sources has yielded a new knowledge in this area as the process development aspects of biomass production have been neglected as an area of research. These studies are very important from the point of media optimization for economic production of Bacillus thuringiensis based insecticides in mosquito control programmes.

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A potential economical substrate for large-scale production ofBacillus thuringiensisvar.kurstakifor caterpillar control

Cited by 4 publications

References 12 publications

Potential of Bacillus thuringiensis in the Management of Pernicious Lepidopteran Pests

Potential of Bacillus thuringiensis in the Management of Pernicious Lepidopteran Pests

The Use of Golden Snail Meal to Enrich Bacillus Thuringiensis Culture Media and Its Effect on the Bacterial Toxicity Against Spodoptera Litura

Evaluation of Different Culture Media for Improvement in Bioinsecticides Production by IndigenousBacillus thuringiensisand Their Application against Larvae ofAedes aegypti

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